The DOI matches its Crossref record; 70 of the 71 references it cites were found in a registry or an index.
RAD51 is Involved in Repair of Damage Associated with DNA Replication in Mammalian Cells
Abstract
The RAD51 protein, a eukaryotic homologue of the Escherichia coli RecA protein, plays an important role in the repair of DNA double-strand breaks (DSBs) by homologous recombination (HR) in mammalian cells. Recent findings suggest that HR may be important in repair following replication arrest in mammalian cells. Here, we have investigated the role of RAD51 in the repair of different types of damage induced during DNA replication with etoposide, hydroxyurea or thymidine. We show that etoposide induces DSBs at newly replicated DNA more frequently than gamma-rays, and that these DSBs are different from those induced by hydroxyurea. No DSB was found following treatment with thymidine. Although these compounds appear to induce different DNA lesions during DNA replication, we show that a cell line overexpressing RAD51 is resistant to all of them, indicating that RAD51 is involved in repair of a wide range of DNA lesions during DNA replication. We observe fewer etoposide-induced DSBs in RAD51-overexpressing cells and that HR repair of etoposide-induced DSBs is faster. Finally, we show that induced long-tract HR in the hprt gene is suppressed in RAD51-overexpressing cells, although global HR appears not to be suppressed. This suggests that overexpression of RAD51 prevents long-tract HR occurring during DNA replication. We discuss our results in light of recent models suggested for HR at stalled replication forks.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“Besides BRCA1, BRCA2, and Rad51 are also required for HR-mediated DNA repair and replication fork maintenance [25, 26].”
From NER initiation factors, DDB2 and XPC, regulate UV radiation response by recruiting ATR and ATM kinases to DNA damage sites (2013) · cites this paper as [25]
In the citing paper
“BRCA1, BRCA2, and Rad51 are known to participate in HR-mediated DNA repair and replication fork maintenance [25, 26].”
From NER initiation factors, DDB2 and XPC, regulate UV radiation response by recruiting ATR and ATM kinases to DNA damage sites (2013) · cites this paper as [25]
Citations
Cites
Genome maintenance mechanisms for preventing cancer
Hoeijmakers, J. H. J. (2001) · Nature · doi:10.1038/35077232 · Cited by 3,939
Martin, S. J., Reutelingsperger, C. P., McGahon, A. J., Rader, J. A., van Schie, R. C., LaFace, D. M., & Green, D. R. (1995) · The Journal of experimental medicine · doi:10.1084/jem.182.5.1545 · Cited by 2,824
Cancer Susceptibility and the Functions of BRCA1 and BRCA2
Venkitaraman, A. R. (2002) · Cell · doi:10.1016/s0092-8674(02)00615-3 · Cited by 1,713
Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis
Sakahira, H., Enari, M., & Nagata, S. (1998) · Nature · doi:10.1038/34214 · Cited by 1,666
The p21 inhibitor of cyclin-dependent kinases controls DNA replication by interaction with PCNA
Waga, S., Hannon, G. J., Beach, D., & Stillman, B. (1994) · Nature · doi:10.1038/369574a0 · Cited by 1,655
Association of BRCA1 with Rad51 in Mitotic and Meiotic Cells
Scully, R., Chen, J., Plug, A., Xiao, Y., Weaver, D., Feunteun, J., Ashley, T., & Livingston, D. M. (1997) · Cell · doi:10.1016/s0092-8674(00)81847-4 · Cited by 1,459
XRCC3 promotes homology-directed repair of DNA damage in mammalian cellsPreprint
Pierce, A. J., Johnson, R. D., Thompson, L. H., & Jasin, M. (1999) · Genes & Development · doi:10.1101/gad.13.20.2633 · Cited by 1,384
Chromosomal stability and the DNA double-stranded break connection
van Gent, D. C., Hoeijmakers, J. H. J., & Kanaar, R. (2001) · Nature Reviews Genetics · doi:10.1038/35056049 · Cited by 1,219
Thelander, L., & Reichard, P. A. (1979) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.48.070179.001025 · Cited by 1,160
Embryonic lethality and radiation hypersensitivity mediated by Rad51 in mice lacking Brca2
Sharan, S. K., Morimatsu, M., Albrecht, U., Lim, D., Regel, E., Dinh, C. Q., Sands, A., Gregor Eichele, C., Hasty, P., & Bradley, A. (1997) · Nature · doi:10.1038/386804a0 · Cited by 1,087
Cited by
Sorted by citations
Items 101–113 of 113
Sugahara, R., Mon, H., Lee, J. M., Shiotsuki, T., & Kusakabe, T. (2014) · FEBS Letters · doi:10.1016/j.febslet.2014.09.009 · Cited by 1
Predicting efficacies of anticancer drugs using single cell HaloChip assay
Ma, L., Xun, X., Qiao, Y., An, J., & Su, M. (2016) · The Analyst · doi:10.1039/c5an02564h · Cited by 1
Roles of Homologous Recombination in Processing DNA Lesions
Tafel, A. A. (2007) · RePub (Erasmus University Rotterdam)
DNA repair by HDR in experimental tumorigenesis
Westermark, U. (2008) · Figshare
Mierau, M. (2005) · Electronic Theses of LMU Munich (Ludwig-Maximilians-Universität München) · doi:10.5282/edoc.4837
Caractérisation et rôle de la phosphorylation de la protéine Rad51
Popova, M. (2009) · OpenGrey (Institut de l'Information Scientifique et Technique)
Support of Mitochondrial DNA Replication by Human Rad51: A Dissertation
Sage, J. M. (2011) · The Journal of the American Medical Association (JAMA) Network (American Medical Association) · doi:10.13028/mj6a-mr98
Luzhna, L. (2009) · Open ULeth Scholarship (OPUS) (University of Lethbridge)
Transcription Associated Recombination in Mammalian Cells
Savolainen, L. (2010) · DiVA (Stockholm University)
MOLECULAR MECHANISMS OF RADIATION-INDUCED BYSTANDER EFFECTS IN VIVO
Koturbash, I. (2008) · Open ULeth Scholarship (OPUS) (University of Lethbridge)
Metadata
- Authors
- Lundin, C., Schultz, N., Arnaudeau, C., Mohindra, A., Hansen, L. T., & Helleday, T.
- Affiliations
- Stockholm University (SE)
- University of Sheffield (GB)
- University of Copenhagen (DK)
- Corresponding author
- Helleday, Thomas
- Venue
- Journal of Molecular Biology
- Publisher
- Elsevier BV
- Published
- 2003-05
- Volume
- 328
- Issue
- 3
- Pages
- 521-535
- ISSN
- 0022-2836 (ISSN-L)
- OpenAlex
- W2052090116
- Cited by
- 113
- References
- 71
- Citations by year
- 2026: 1 · 2024: 4 · 2023: 3 · 2022: 2 · 2021: 3 · 2020: 2 · 2019: 2 · 2018: 3 · 2017: 4 · 2016: 4 · 2015: 8 · 2014: 4 · 2013: 5 · 2012: 4
- FWCI
- 3.79
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Molecular Biology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Stiftelsen Forska Utan Djurförsök
- Cancerfonden
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Effects of Radiation Exposure
- Health Sciences › Medicine › Oncology › PARP inhibition in cancer therapy
- Keywords
- RAD51, homologous recombination, DNA double-strand break repair, replication arrest, etoposide, hydroxyurea, thymidine, HPRT gene, stalled replication forks
- MeSH terms
- Animals, Cell Line, DNA Damage, DNA Repair, DNA Replication, DNA-Binding Proteins, Dose-Response Relationship, Drug, Etoposide, Cricetinae, Hydroxyurea, Hypoxanthine Phosphoribosyltransferase, Kinetics, Models, Genetic, Recombination, Genetic, Thymidine, Apoptosis, Rad51 Recombinase
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
