Cited by 336Multiple Sclerosis Research StudiesHereditary Neurological DisordersNeurogenesis and neuroplasticity mechanisms

Chromosomal mapping of mouse myelin basic protein gene and structure and transcription of the partially deleted gene in shiverer mutant mice

ROACH, A, TAKAHASHI, N, PRAVTCHEVA, D, RUDDLE, F, HOOD, L·1985·Cell

Abstract

The gene for mouse myelin basic protein (MBP) was mapped to chromosome 18 by hybridization of cloned MBP probes to DNA from hamster-mouse hybrid cell lines, showing it to be linked to the shiverer mutation which causes abnormal CNS myelination. Genomic blotting experiments show that in the mutants five of six exons which constitute the wild-type gene have been deleted. In shiverer brains the steady state level of transcripts that initiate correctly at the 5' end of the remaining exon 1 is reduced 16-fold. These RNAs are not spliced correctly and are not efficiently polyadenylated. It is proposed that the partial deletion of the MBP gene is an important part of the shiverer lesion.

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Crossref · 10.1016/s0092-8674(85)80110-0

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Authors
ROACH, A., TAKAHASHI, N., PRAVTCHEVA, D., RUDDLE, F., & HOOD, L.
Affiliations
  • California Institute of Technology (US)
  • Yale University (US)
ORCID
HOOD, L
Venue
Cell
Publisher
Elsevier BV
Published
1985-08
Volume
42
Issue
1
Pages
149-155
ISSN
0092-8674 (ISSN-L)
OpenAlex
W2151814085
Cited by
336
References
32
Citations by year
2025: 5 · 2024: 2 · 2023: 3 · 2022: 5 · 2021: 6 · 2020: 3 · 2019: 3 · 2018: 6 · 2017: 4 · 2016: 13 · 2015: 6 · 2014: 5 · 2013: 6 · 2012: 3
FWCI
21.88
Type
journal article
Language
en
Open access
closed
Locations
  • Cell (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Institutes of Health
Topics
  • Health Sciences › Medicine › Pathology and Forensic Medicine › Multiple Sclerosis Research Studies
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Hereditary Neurological Disorders
  • Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
Keywords
myelin basic protein, chromosome 18, gene mapping, partial gene deletion, CNS myelination, alternative splicing, transcription initiation, polyadenylation, exon deletion
MeSH terms
Animals, Base Sequence, Brain, Chromosome Deletion, Chromosome Mapping, Myelin Basic Protein, Genes, Genetic Linkage, Mice, Neurologic Mutants, Mutation, Poly A, RNA Splicing, RNA, Messenger, Transcription, Genetic, Mice

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