Immunoreactive orexin-A in human plasma
Abstract
Orexin-A and orexin-B are newly discovered neuropeptides which are implicated in feeding behavior and arousal state. We studied immunoreactive(IR)-orexin-A concentrations in human plasma by radioimmunoassay. IR-orexin-A concentrations in plasma obtained from 17 healthy subjects in the morning were 1.94 +/- 0.24 pmol/liter (mean +/- SEM). IR-orexin-A levels in the plasma obtained at night were not significantly different from those obtained in the morning in 9 female subjects. The HPLC analysis of the plasma extract showed two immunoreactive peaks; one peak eluting in an identical position to synthetic orexin-A, and one eluting earlier. This study has shown for the first time the presence of orexin-A in human plasma.
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Prior work to measure stress-related biomarkers has utilized high-performance liquid chromatography (HPLC), enzyme-linked immunosorbent assay, radioimmunoassay kit, or a HPLC mass spectrometry (MS) system.
“Prior work to measure stress-related biomarkers has utilized high-performance liquid chromatography (HPLC),99 enzyme-linked immunosorbent assay,100 radioimmunoassay kit,101 or a HPLC mass spectrometry (MS) system.102”
From Wearable sensors for monitoring the physiological and biochemical profile of the athlete (2019) · cites this paper as [99]
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Wearable sensors for monitoring the physiological and biochemical profile of the athlete
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- Authors
- Arihara, Z., Takahashi, K., Murakami, O., Totsune, K., Sone, M., Satoh, F., Ito, S., & Mouri, T.
- Affiliations
- Tohoku University (JP)
- Corresponding author
- Takahashi, Kazuhiro
- Venue
- Peptides
- Publisher
- Elsevier BV
- Published
- 2001-01
- Volume
- 22
- Issue
- 1
- Pages
- 139-142
- ISSN
- 0196-9781 (ISSN-L)
- OpenAlex
- W2019378961
- Cited by
- 101
- References
- 23
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 1 · 2023: 2 · 2022: 6 · 2021: 3 · 2020: 1 · 2019: 6 · 2018: 5 · 2017: 1 · 2015: 5 · 2014: 2 · 2013: 5 · 2012: 7
- FWCI
- 3.49
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Peptides (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Takeda Science Foundation
- Topics
- Life Sciences › Neuroscience › Cognitive Neuroscience › Sleep and Wakefulness Research
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
- Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Regulation of Appetite and Obesity
- Keywords
- orexin-A, orexin B, human plasma, neuropeptides, circadian variation, feeding behavior, arousal, plasma peptide levels
- MeSH terms
- Orexins, Adult, Carrier Proteins, Chromatography, High Pressure Liquid, Female, Humans, Male, Middle Aged, Neuropeptides, Radioimmunoassay, Intracellular Signaling Peptides and Proteins
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