Detection of Orexin A Neuropeptide in Biological Fluids Using a Zinc Oxide Field Effect Transistor
Open accessAbstract
Biomarkers which are indicative of acute physiological and emotional states are studied in a number of different areas in cognitive neuroscience. Currently, many cognitive studies are conducted based on programmed tasks followed by timed biofluid sampling, central laboratory processing, and followed by data analysis. In this work, we present a sensor platform capable of rapid biomarker detection specific for detecting neuropeptide orexin A, found in blood and saliva and known as an indicator of fatigue and cognitive performance. A peptide recognition element that selectively binds to orexin A was designed, characterized, and functionalized onto a zinc oxide field effect transistor to enable rapid detection. The detection limit using the sensor platform was sub-picomolar in water, and picomolar to nanomolar levels in saliva and serum. The transistor and recognition element sensor platform can be easily expanded, allowing for multiple biomarkers to be detected simultaneously, lending itself to complex biomarker analysis applicable to rapid feedback for neuroscience research and physiological monitoring.
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Development of improved sensitivity and stability of biosensors is required for the real-time detection of such biomarkers.
“The sensor platform demonstrated the potential of an FET device to measure a wide array of biomarkers in complex biofluids; however, development of improved sensitivity and stability of biosensors is required for the real-time detection of such biomarkers.113”
From Wearable sensors for monitoring the physiological and biochemical profile of the athlete (2019) · cites this paper as [113]
The sensor platform demonstrated the potential of an FET device to measure a wide array of biomarkers in complex biofluids.
“The sensor platform demonstrated the potential of an FET device to measure a wide array of biomarkers in complex biofluids; however, development of improved sensitivity and stability of biosensors is required for the real-time detection of such biomarkers.113”
From Wearable sensors for monitoring the physiological and biochemical profile of the athlete (2019) · cites this paper as [113]
Orexin peptides and their corresponding receptors in the brain contribute to autonomic control, attention, feeding, memory, sleep, and stress.
“Orexin peptides and their corresponding receptors in the brain contribute to autonomic control, attention, feeding, memory, sleep, and stress.113”
From Wearable sensors for monitoring the physiological and biochemical profile of the athlete (2019) · cites this paper as [113]
Measuring serum levels of Orexin A can aid in predicting mood and cognitive performance in athletes.
“Measuring serum levels of Orexin A can aid in predicting mood and cognitive performance in athletes.113”
From Wearable sensors for monitoring the physiological and biochemical profile of the athlete (2019) · cites this paper as [113]
The binding was transmitted to an electrical signal.
“The binding was transmitted to an electrical signal”
From Wearable sensors for monitoring the physiological and biochemical profile of the athlete (2019) · cites this paper as [113]
Prior work using biosensors to measure stress and cognition has focused on the measurement of a wide array of biomarkers (10−6-10−12 M) such as Orexin-A, cortisol, dopamine, neuropeptide Y, and interleukin-6 (IL-6).
“Prior work using biosensors to measure stress and cognition has focused on the measurement of a wide array of biomarkers (10−6–10−12 M) such as Orexin-A,113 cortisol,110 dopamine,114 neuropeptide Y,115 and interleukin-6 (IL-6).116”
From Wearable sensors for monitoring the physiological and biochemical profile of the athlete (2019) · cites this paper as [113]
The sensor selectivity was able to detect concentrations of approximately 100 aM in water, 10 fM in filtered human saliva, and 1 nM in filtered fetal bovine serum.
“The binding was transmitted to an electrical signal and the sensor selectivity was able to detect concentrations of approximately 100 aM in water, 10 fM in filtered human saliva, and 1 nM in filtered fetal bovine serum.”
From Wearable sensors for monitoring the physiological and biochemical profile of the athlete (2019) · cites this paper as [113]
Hagen et al. utilized interdigitated zinc oxide field effect transistors (ZnO FETs) to detect Orexin A by binding a bifunctional peptide to both the ZnO semiconductor and the neuropeptide.
“Hagen et al. utilized interdigitated zinc oxide field effect transistors (ZnO FETs) to detect Orexin A by binding a bifunctional peptide to both the ZnO semiconductor and the neuropeptide.113”
From Wearable sensors for monitoring the physiological and biochemical profile of the athlete (2019) · cites this paper as [113]
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Sorted by citations
Wearable sensors for monitoring the physiological and biochemical profile of the athlete
Seshadri, D. R., Li, R. T., Voos, J. E., Rowbottom, J. R., Alfes, C. M., Zorman, C. A., & Drummond, C. K. (2019) · npj Digital Medicine · doi:10.1038/s41746-019-0150-9 · Cited by 537 · see citing works
- Authors
- Hagen, J., Lyon, W., Chushak, Y., Tomczak, M., Naik, R., Stone, M., & Kelley-Loughnane, N.
- Affiliations
- United States Air Force Research Laboratory (US)
- 711th Human Performance Wing, Human Effectiveness Directorate and ‡Materials and Manufacturing Directorate, Air Force Research Laboratories, Wright Patterson Air Force Base, Dayton, Ohio, United States
- Corresponding author
- Hagen, Joshua
- Venue
- ACS Chemical Neuroscience
- Publisher
- American Chemical Society (ACS)
- Published
- 2013-01-22
- Volume
- 4
- Issue
- 3
- Pages
- 444-453
- ISSN
- 1948-7193 (ISSN-L)
- PMC
- PMC3605810
- OpenAlex
- W1996359578
- Cited by
- 22
- References
- 42
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 1 · 2023: 2 · 2022: 2 · 2021: 3 · 2020: 2 · 2019: 4 · 2017: 2 · 2016: 2 · 2015: 2
- FWCI
- 0.80
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- ACS Chemical Neuroscience (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Air Force Research Laboratory
- Materials and Manufacturing Directorate
- Topics
- Physical Sciences › Engineering › Electrical and Electronic Engineering › Electrochemical sensors and biosensors
- Physical Sciences › Chemical Engineering › Bioengineering › Analytical Chemistry and Sensors
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced biosensing and bioanalysis techniques
- Keywords
- orexin-A, biomarker detection, saliva, serum, fatigue, cognitive performance, biosensors, physiological monitoring
- MeSH terms
- Orexins, Amino Acid Sequence, Animals, Humans, Molecular Sequence Data, Neuropeptides, Saliva, Transistors, Electronic, Zinc Oxide, Biosensing Techniques, Biomarkers, Serum, Intracellular Signaling Peptides and Proteins, Mice, Rats
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