Effects of estrogen on the expression of connexin32 and connexin43 mRNAs in the suprachiasmatic nucleus of female rats
Abstract
The suprachiasmatic nucleus of the hypothalamus (SCN) is the site of the endogenous biological clock that regulates circadian rhythms in mammals. It has been shown that the gap junction couples neurons in the rat SCN. To determine the effects of estrogen on gap junction communication in the SCN, we examined the effects of estrogen on the expression of connexin32 and connexin43 mRNAs in the SCN and cerebral cortex (CX) of female rats. Ovarectomized adult rats were injected with 20 microg of 17beta-estradiol and killed 24 h after the treatment. Northern blot revealed that the expression of connexin32 mRNA was significantly increased in the SCN but not in the CX. On the other hand, estrogen increased the connexin43 mRNA level in the CX but not in the SCN. These results suggest that the gap junction with connexin32 is specifically regulated by estrogen in the SCN of female rats.
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A functional analysis of circadian pacemakers in nocturnal rodents
Pittendrigh, C. S., & Daan, S. (1976) · Journal of Comparative Physiology A · doi:10.1007/bf01417856 · Cited by 2,129 · see citing works
Pittendrigh, C. S., & Daan, S. (1976) · Journal of Comparative Physiology ? A · doi:10.1007/bf01417860 · Cited by 1,636 · see citing works
Welsh, D. K., Logothetis, D., Meister, M., & Reppert, S. (1995) · Neuron · doi:10.1016/0896-6273(95)90214-7 · Cited by 1,535 · see citing works
Connexin43: a protein from rat heart homologous to a gap junction protein from liver.
Beyer, E. C., Paul, D. L., & Goodenough, D. A. (1987) · The Journal of Cell Biology · doi:10.1083/jcb.105.6.2621 · Cited by 1,117 · see citing works
pl Bl5: A cDNA Clone of the Rat mRNA Encoding Cyclophilin
Danielson, P. E., Forss‐Petter, S., Brow, M. A. D., Calavetta, L., DOUGLASS, J. O., Milner, R. J., & Sutcliffe, J. G. (1988) · DNA · doi:10.1089/dna.1988.7.261 · Cited by 1,020 · see citing works
Neurophysiology of the suprachiasmatic circadian pacemaker in rodents
Meijer, J. H., & Rietveld, W. J. (1989) · Physiological Reviews · doi:10.1152/physrev.1989.69.3.671 · Cited by 737 · see citing works
Molecular cloning of cDNA for rat liver gap junction protein.
Paul, D. L. (1986) · The Journal of Cell Biology · doi:10.1083/jcb.103.1.123 · Cited by 695 · see citing works
Gap junctions in the brain: where, what type, how many and why?
Dermietzel, R., & Spray, D. C. (1993) · Trends in Neurosciences · doi:10.1016/0166-2236(93)90151-b · Cited by 501 · see citing works
Estradiol shortens the period of hamster circadian rhythms
Morin, L. P., Fitzgerald, K. M., & Zucker, I. (1977) · Science · doi:10.1126/science.557840 · Cited by 350 · see citing works
Two distinct oscillators in the rat suprachiasmatic nucleus in vitro.
Shinohara, K., Honma, S., Katsuno, Y., Abe, H., & Honma, K.-I. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.16.7396 · Cited by 215 · see citing works
Self-citationMicevych, P. E., & Abelson, L. (1991) · The Journal of Comparative Neurology · doi:10.1002/cne.903050110 · Cited by 175 · see citing works
Gonadal hormones organize and modulate the circadian system of the rat
Albers, H. E. (1981) · American Journal of Physiology-Regulatory, Integrative and Comparative Physiology · doi:10.1152/ajpregu.1981.241.1.r62 · Cited by 168 · see citing works
Cellular communication in the circadian clock, the suprachiasmatic nucleus
van den Pol, A. N., & Dudek, F. E. (1993) · Neuroscience · doi:10.1016/0306-4522(93)90128-3 · Cited by 146 · see citing works
Autoradiographic Localization of Estradiol-Concentrating Cells in the Female Hamster Brain
Krieger, M. S., Morrell, J. I., & Pfaff, D. W. (2008) · Neuroendocrinology · doi:10.1159/000122626 · Cited by 115 · see citing works
Properties of Human Sleep-Wake Cycles: Parameters of Internally Synchronized Free-Running Rhythms
Wever, R. (1984) · SLEEP · doi:10.1093/sleep/7.1.27 · Cited by 93 · see citing works
Effect of ovarian hormones on synchrony of hamster circadian rhythms
Morin, L. P. (1980) · Physiology & Behavior · doi:10.1016/0031-9384(80)90406-0 · Cited by 92 · see citing works
Tracer and electrical coupling of rat suprachiasmatic nucleus neurons
Jiang, Z.-G., Yang, Y.-Q., & Allen, C. N. (1997) · Neuroscience · doi:10.1016/s0306-4522(96)00539-8 · Cited by 67 · see citing works
Shinohara, K., Hiruma, H., Funabashi, T., & Kimura, F. (2000) · Neuroscience · doi:10.1016/s0306-4522(99)00556-4 · Cited by 59 · see citing works
Self-citationExpression of gap junction genes during postnatal neural development
Belliveau, D. J., Kidder, G. M., & Naus, C. C. G. (1991) · Developmental Genetics · doi:10.1002/dvg.1020120408 · Cited by 53 · see citing works
Funabashi, T., Brooks, P. J., Kleopoulos, S. P., Grandison, L., Mobbs, C. V., & Pfaff, D. W. (1995) · Molecular Brain Research · doi:10.1016/0169-328x(94)00191-g · Cited by 42 · see citing works
Self-citationShinohara, K., Uchiyama, M., Okawa, M., Saito, K., Kawaguchi, M., Funabashi, T., & Kimura, F. (2000) · Neuroscience Letters · doi:10.1016/s0304-3940(00)00826-0 · Cited by 26 · see citing works
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The neuroendocrine control of the circadian system: Adolescent chronotype
Hagenauer, M. H., & Lee, T. M. (2012) · Frontiers in Neuroendocrinology · doi:10.1016/j.yfrne.2012.04.003 · Cited by 143 · see citing works
Minireview: Timely Ovulation: Circadian Regulation of the Female Hypothalamo-Pituitary-Gonadal Axis
de la Iglesia, H. O., & Schwartz, W. J. (2006) · Endocrinology · doi:10.1210/en.2005-1311 · Cited by 134 · see citing works
Vida, B., Hrabovszky, E., Kalamatianos, T., Coen, C. W., Liposits, Z., & Kalló, I. (2008) · Journal of Neuroendocrinology · doi:10.1111/j.1365-2826.2008.01787.x · Cited by 108 · see citing works
Estradiol enhances light-induced expression of transcription factors in the SCN
Abizaid, A., Mezei, G., & Horvath, T. L. (2004) · Brain Research · doi:10.1016/j.brainres.2004.01.089 · Cited by 44 · see citing works
Shinohara, K., Funabashi, T., Nakamura, T. J., & Kimura, F. (2001) · Neuroscience Letters · doi:10.1016/s0304-3940(01)02022-5 · Cited by 43 · see citing works
Self-citationTime for testosterone: the suprachiasmatic nucleus gets sexy
Hagenauer, M. H., & Lee, T. M. (2011) · Endocrinology · doi:10.1210/en.2011-0198 · Cited by 12 · see citing works
- Authors
- Shinohara, K., Funabashi, T., Mitushima, D., & Kimura, F.
- Affiliations
- Yokohama City University (JP)
- Corresponding author
- Shinohara, Kazuyuki
- Venue
- Neuroscience Letters
- Publisher
- Elsevier BV
- Published
- 2000-06
- Volume
- 286
- Issue
- 2
- Pages
- 107-110
- ISSN
- 0304-3940 (ISSN-L)
- OpenAlex
- W2003488578
- Cited by
- 25
- References
- 21
- Citations by year
- 2021: 1 · 2017: 1 · 2013: 2 · 2012: 3
- FWCI
- 0.72
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Neuroscience Letters (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Connexins and lens biology
- Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Circadian rhythm and melatonin
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
- Keywords
- estrogen, connexin 32, connexin 43, suprachiasmatic nucleus, gap junctions, circadian rhythm, estradiol, ovariectomy, cerebral cortex, mRNA expression, female rats
- MeSH terms
- Gap Junction beta-1 Protein, Animals, Cerebral Cortex, Circadian Rhythm, Estrogens, Female, Neurons, RNA, Messenger, Suprachiasmatic Nucleus, Blotting, Northern, Rats, Wistar, Gap Junctions, Connexins, Connexin 43, Reverse Transcriptase Polymerase Chain Reaction, Rats
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