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Cited by 44Circadian rhythm and melatoninHypothalamic control of reproductive hormonesEstrogen and related hormone effects

Estradiol enhances light-induced expression of transcription factors in the SCN

Abizaid, Alfonso, Mezei, Gabor, Horvath, Tamas L·2004·Brain Research
DOI 10.1016/j.brainres.2004.01.089
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Abstract

The suprachiasmatic nucleus of the hypothalamus (SCN) is the master clock that regulates circadian and seasonal rhythms. Among these, the SCN regulates the phasic release of hormones and provides for the timing of the preovulatory luteinizing hormone (LH) surge necessary for ovulation in females. There is little evidence, however, of sex hormone effects on mechanisms underlying SCN function. This study examined the effects of exogenous administration of estradiol on the light-induced expression of transcription factors in the SCN of female rats. Ovariectomized (OVX) female rats were given estradiol or cholesterol implants and perfused 48 h later. Half of the animals were sacrificed 1 h after the regular onset of light within the colony. The rest had the lights go on 2 h prior to the regular time and perfused 1 h later. Collected brains were sliced and sets of SCN sections were processed for immunoreactivity (ir) detecting the Fos, pCREB, egr-1, CREB binding protein (CBP), and calbindin-D (28K) proteins. Following quantification, statistical analyses demonstrated that estradiol enhanced Fos and p-CREB-ir in the SCN of females that experienced a 2-h phase advance. The phase advance also enhanced calbindin and egr-1-ir, but the expression of these proteins was not affected by estradiol. These results demonstrate that estradiol enhances the levels of transcription factors that precede the expression of clock gene proteins in the SCN in response to advances in the onset of environmental light. These data support the hypothesis that steroid hormones play an important role in the fine tuning of the clock in the face of environmental changes in daylight.

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  • Both androgens and estrogens can activationally alter photic sensitivity within the entrainment pathway, as measured by the induction of FOS and clock genes (Per1, Per2).

    “For example, both androgens and estrogens can activationally alter photic sensitivity within the entrainment pathway, as measured by the induction of immediate early gene expression (FOS) and clock genes (Per1, Per2, [3, 88, 90]).”

    From The neuroendocrine control of the circadian system: Adolescent chronotype (2012) · cites this paper as [3]

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Authors
Abizaid, A., Mezei, G., & Horvath, T. L.
Affiliations
Yale University (US)
ORCID
Abizaid, Alfonso
Venue
Brain Research
Publisher
Elsevier BV
Published
2004-06
Volume
1010
Issue
1-2
Pages
35-44
ISSN
0006-8993 (ISSN-L)
DOI
10.1016/j.brainres.2004.01.089
OpenAlex
W2073873596
Cited by
44
References
69
Citations by year
2026: 1 · 2025: 2 · 2024: 2 · 2023: 1 · 2021: 1 · 2020: 5 · 2019: 1 · 2018: 1 · 2017: 2 · 2015: 1 · 2013: 3 · 2012: 2
FWCI
0.93
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Brain Research (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Institute of Mental Health (R01MH059847, MH059847)
Topics
  • Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Circadian rhythm and melatonin
  • Health Sciences › Medicine › Reproductive Medicine › Hypothalamic control of reproductive hormones
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
Keywords
estradiol, suprachiasmatic nucleus, circadian clock, light-induced gene expression, transcription factors, FOS, phosphorylated CREB, EGR1, CREB-binding protein, calbindin-D28k, phase advance, steroid hormones, clock gene expression, female rats
MeSH terms
Animals, Cholesterol, Circadian Rhythm, DNA-Binding Proteins, Estradiol, Female, Light, Nuclear Proteins, Ovariectomy, Photic Stimulation, Suprachiasmatic Nucleus, Transcription Factors, Trans-Activators, Up-Regulation, Proto-Oncogene Proteins c-fos, Rats, Sprague-Dawley, Cyclic AMP Response Element-Binding Protein, Immediate-Early Proteins, Estrous Cycle, CREB-Binding Protein, Rats, Early Growth Response Protein 1, Calbindins, S100 Calcium Binding Protein G

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