Gluthatione level is altered in lymphoblasts from patients with familial Alzheimer’s disease
Abstract
Intracellular levels of glutathione (GSH), glutathione disulphide (GSSG), glutamic acid and gamma-glutamyl cysteine synthetase (gamma-GCS) were measured in lymphoblast lines from patients with familial and sporadic Alzheimer's disease (AD) and from age-matched controls. Lymphoblasts carrying presenilins (PS) and amyloid precursor protein (APP) genes mutations showed significantly decreased GSH content with respect to controls. Levels of GSSG and glutamic acid, as well as the activity of gamma-GCS were not significantly different in lymphoblasts carrying genes mutations as compared with control cells. These results indicate that even peripheral cells not involved in the neurodegenerative process of AD show altered GSH content when carrying PS and APP genes mutations. The provided data appear to be in accordance with the known alteration of GSH levels in central nervous system and strengthen the hypothesis of oxidative stress as an important, possibly crucial mechanism in the pathogenesis of AD.
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GCLC is the main regulating protein for glutathione production, a process impacted by AD pathology.
“GCLC is the main regulating protein for glutathione production, a process impacted by AD pathology [24,25,26].”
From Centella Asiatica Improves Memory and Promotes Antioxidative Signaling in 5XFAD Mice (2019) · cites this paper as [26]
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- Authors
- Cecchi, C., Latorraca, S., Sorbi, S., Iantomasi, T., Favilli, F., Vincenzini, M. T., & Liguri, G.
- Affiliations
- University of Florence (IT)
- Corresponding author
- Liguri, Gianfranco
- Venue
- Neuroscience Letters
- Publisher
- Elsevier BV
- Published
- 1999-11
- Volume
- 275
- Issue
- 2
- Pages
- 152-154
- ISSN
- 0304-3940 (ISSN-L)
- OpenAlex
- W2088881207
- Cited by
- 101
- References
- 20
- Citations by year
- 2024: 2 · 2023: 1 · 2022: 1 · 2021: 4 · 2020: 2 · 2019: 4 · 2018: 2 · 2017: 3 · 2016: 3 · 2015: 4 · 2014: 6 · 2013: 1 · 2012: 8
- FWCI
- 1.89
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Neuroscience Letters (publishedVersion)
- PubMed (publishedVersion)
- Florence Research (University of Florence) (submittedVersion)
- Topics
- Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Glutathione Transferases and Polymorphisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Sulfur Compounds in Biology
- Keywords
- glutathione, glutathione disulfide, gamma-glutamylcysteine synthetase, familial Alzheimer's disease, presenilin mutations, amyloid precursor protein mutations, lymphoblasts, oxidative stress, glutamate, glutathione metabolism
- MeSH terms
- Alzheimer Disease, Glutamate-Cysteine Ligase, Glutathione, Humans, Lymphocyte Activation, Lymphocytes, Membrane Proteins, Oxidation-Reduction, Amyloid beta-Protein Precursor, Glutamic Acid, Glutathione Disulfide, Presenilin-1
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