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Cited by 9Parasitic Diseases Research and TreatmentHelminth infection and controlResearch on Leishmaniasis Studies

Utility of adult antigens of Dirofilaria immitis for the early detection of dirofilariosis and for the evaluation of chemoprophylactic treatment in experimentally infected cats

Prieto, G., Simón, F., Genchi, C., McCall, J.W., Venco, L.·1999·Veterinary Parasitology
DOI 10.1016/s0304-4017(99)00116-8
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Abstract

Early detection of feline dirofilariosis was achieved by ELISA testing using adult somatic (SA) and excretory/secretory (E/S) antigens. Sera from experimentally infected cats showed significantly higher IgG levels at 2 months post-infection (p.i.) when compared to sera obtained prior to infection. This difference was apparent at all serum dilutions (1:100 to 1:800) in ELISA(SA) and at all dilutions except 1:800 when tested with ELISA(E/S). Changes in specific anti-SA and anti-E/S IgG levels were found in infected cats treated with the macrolid parasiticide. ELISA(E/S) was able to detect a significant decrease in IgG levels in ivermectin treated cats at 3 months post-treatment (p.t.), compared to infected, untreated controls. However, testing with ELISA(SA) showed a significant decrease in antibody levels in treated cats only at 4 months after treatment. Sera from untreated cats recognized previously identified specific markers for feline dirofilariosis on SA (22, 26, 30 and 40 kDa) and on E/S (22 and 25 kDa) in Western blot analysis. Sera from treated cats, on the other hand, gave only weak bands or none at all, indicating a decrease in antibody concentration. These results suggest that serologic testing with ELISA is useful in the early detection of Dirofilaria immitis infection and, together with Western blot analysis, can assist in the evaluation of response to chemoprophylactic treatment of feline dirofilariosis, two aspects of prime importance in the prevention of this disease in cats.

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Crossref · 10.1016/s0304-4017(99)00116-8

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  • Immune response in feline cardiopulmonary dirofilariasis is generally strong after approximately 2 m.p.i., enabling early detection of single-worm infections.

    “The immune response in feline cardiopulmonary dirofilariasis is generally strong after approximately 2 m.p.i., thereby enabling the early detection of single-worm infections (346,348).”

    From Human and animal dirofilariasis: The emergence of a zoonotic mosaic (2012) · cites this paper as [348]

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Authors
Prieto, G., Simón, F., Genchi, C., McCall, J. W., & Venco, L.
Affiliations
  • Universidad de Salamanca (ES)
  • University of Milan (IT)
  • University of Georgia (US)
ORCID
Venue
Veterinary Parasitology
Publisher
Elsevier BV
Published
1999-09
Volume
86
Issue
1
Pages
5-13
ISSN
0304-4017 (ISSN-L)
DOI
10.1016/s0304-4017(99)00116-8
OpenAlex
W1996815763
Cited by
9
References
16
Citations by year
2022: 1 · 2014: 1 · 2012: 1
FWCI
1.23
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Veterinary Parasitology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Infectious Diseases › Parasitic Diseases Research and Treatment
  • Health Sciences › Veterinary › Small Animals › Helminth infection and control
  • Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Research on Leishmaniasis Studies
Keywords
Dirofilaria immitis, excretory-secretory antigens, serological diagnosis, early infection detection, chemoprophylaxis, ivermectin, IgG antibody response, cats
MeSH terms
Animals, Anthelmintics, Antibodies, Helminth, Antigens, Helminth, Cat Diseases, Cats, Dirofilaria immitis, Dirofilariasis, Enzyme-Linked Immunosorbent Assay, Female, Heart, Ivermectin, Lung, Male, Blotting, Western

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