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Incorporation of the elderberry anthocyanins by endothelial cells increases protection against oxidative stress
Abstract
The objective of this study was to investigate the ability of endothelial cells (EC) to incorporate anthocyanins and to examine their potential benefits against various oxidative stressors. Endothelial dysfunction has been proposed to play an important role in the initiation and development of vascular disease, with studies having shown that administration of antioxidants improves endothelial function. Elderberry extract contains 4 anthocyanins, which where incorporated into the plasma membrane and cytosol of EC following 4 h incubation at 1 mg.ml(-1). However, incorporation within the cytosol was considerably less than that in the membrane. Uptake within both regions appeared to be structure dependent, with monoglycoside concentrations higher than that of the diglucosides in both compartments. The enrichment of EC with elderberry anthocyanins conferred significant protective effects in EC against the following oxidative stressors: hydrogen peroxide (H(2)O(2)); 2, 2'-azobis(2-amidinopropane) dihydrochloride (AAPH); and FeSO(4)/ascorbic acid (AA). These results show for the first time that vascular EC can incorporate anthocyanins into the membrane and cytosol, conferring significant protective effects against oxidative insult. These findings may have important implications on preserving EC function and preventing the initiation of EC changes associated with vascular diseases.
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“Youdim et al. have reported the incorporation of elderberry anthocyanins by endothelial cells, following a 4-h incubation at a concentration of 1 mg/mL.”
From Berries: emerging impact on cardiovascular health (2010) · cites this paper as [60]
In the citing paper
“Several lines of evidence indicate a role for berry anthocyanins in significantly decreasing oxidative damage and inflammation in cellular and animal models of CVD.”
From Berries: emerging impact on cardiovascular health (2010) · cites this paper as [60]
In the citing paper
“In addition to the cellular bioavailability, elderberry anthocyanins significantly decreased cytotoxicity caused by chemical inducers of oxidative stress.”
From Berries: emerging impact on cardiovascular health (2010) · cites this paper as [60]
Citations
Cites
Mosmann, T. (1983) · Journal of Immunological Methods · doi:10.1016/0022-1759(83)90303-4 · Cited by 56,104
Free Radicals as Mediators of Tissue Injury and Disease
Kehrer, J. P. (1993) · Critical Reviews in Toxicology · doi:10.3109/10408449309104073 · Cited by 1,460
Joseph, J. A., Shukitt-Hale, B., Denisova, N. A., Bielinski, D., Martin, A., McEwen, J. J., & Bickford, P. C. (1999) · The Journal of Neuroscience · doi:10.1523/jneurosci.19-18-08114.1999 · Cited by 980
Self-citationRedox Regulation of NF-kappa B Activation
Flohé, L., Brigelius‐Flohé, R., Saliou, C., Traber, M. G., & Packer, L. (1997) · Free Radical Biology and Medicine · doi:10.1016/s0891-5849(96)00501-1 · Cited by 859
Taddei, S., Virdis, A., Ghiadoni, L., Magagna, A., & Salvetti, A. (1998) · Circulation · doi:10.1161/01.cir.97.22.2222 · Cited by 757
Effect of Plant Flavonoids on Immune and Inflammatory Cell Function
Middleton, E. (1998) · Advances in experimental medicine and biology · doi:10.1007/978-1-4615-5335-9_13 · Cited by 735
Day, A. J., DuPont, M. S., Ridley, S., Rhodes, M. J. C., Rhodes, M. J. C., Morgan, M. R. A., & Williamson, G. (1998) · FEBS Letters · doi:10.1016/s0014-5793(98)01101-6 · Cited by 730
Effects of flavonoids on immune and inflammatory cell functions
Middleton, E., & Kandaswami, C. C. (1992) · Biochemical Pharmacology · doi:10.1016/0006-2952(92)90489-6 · Cited by 675
Tsai, S., Lin‐Shiau, S., & Lin, J. (1999) · British Journal of Pharmacology · doi:10.1038/sj.bjp.0702357 · Cited by 511
Synthesis of von Willebrand Factor by Cultured Human Endothelial Cells
JAFFE, E. A., Hoyer, L. W., & Nachman, R. L. (1974) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.71.5.1906 · Cited by 485
Kuntz, S., Wenzel, U., & Daniel, H. (1999) · European Journal of Nutrition · doi:10.1007/s003940050054 · Cited by 484
Intestinal absorption of luteolin and luteolin 7‐O‐β‐glucoside in rats and humans
Shimoi, K., Okada, H., Furugori, M., Goda, T., Takase, S., Suzuki, M., Hara, Y., Yamamoto, H., & Kinae, N. (1998) · FEBS Letters · doi:10.1016/s0014-5793(98)01304-0 · Cited by 418
Miyazawa, T., Nakagawa, K., Kudo, M., Kayo, M., & Someya, K. (1999) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf9809582 · Cited by 368
Absorption and metabolism of cyanidin 3‐ O ‐β‐ D ‐glucoside in rats
Tsuda, T., Horio, F., & Osawa, T. (1999) · FEBS Letters · doi:10.1016/s0014-5793(99)00407-x · Cited by 354
Association of circulating TNF-α and IL-6 with ageing and parkinsonism
Dobbs, R. J., Charlett, A., Purkiss, A. G., Dobbs, S. M., Weller, C., & Peterson, D. W. (1999) · Acta Neurologica Scandinavica · doi:10.1111/j.1600-0404.1999.tb00721.x · Cited by 324
Flavonoids inhibit cytokine-induced endothelial cell adhesion protein gene expression.
Gerritsen, M. E., Carley, W. W., Ranges, G. E., Shen, C. P., Phan, S. A., Ligon, G. F., & Perry, C. A. (1995) · Europe PMC (PubMed Central) · Cited by 305
The role of nitric oxide and other endothelium-derived vasoactive substances in vascular disease
Cohen, R. A. (1995) · Progress in Cardiovascular Diseases · doi:10.1016/s0033-0620(05)80002-7 · Cited by 284
Vitamin C Improves Endothelial Dysfunction of Epicardial Coronary Arteries in Hypertensive Patients
Solzbach, U., Hornig, B., Jeserich, M., & Just, H. (1997) · Circulation · doi:10.1161/01.cir.96.5.1513 · Cited by 278
In VitroAnticancer Activity of Fruit Extracts fromVacciniumSpecies
Bomser, J. A., Madhavi, D., Singletary, K. W., & Smith, M. A. (1996) · Planta Medica · doi:10.1055/s-2006-957862 · Cited by 277
Gee, J. M., DuPont, M. S., Rhodes, M. J. C., & Johnson, I. T. (1998) · Free Radical Biology and Medicine · doi:10.1016/s0891-5849(98)00020-3 · Cited by 266
Effects of fluid dynamic forces on vascular cell adhesion.
Κωνσταντόπουλος, Κ., & McIntire, L. V. (1996) · Journal of Clinical Investigation · doi:10.1172/jci119088 · Cited by 261
Absorption and Distribution of Tea Catechin(-)-Epigallocatechin-3-Gallate, in the Rat.
Nakagawa, K., & Miyazawa, T. (1997) · Journal of Nutritional Science and Vitaminology · doi:10.3177/jnsv.43.679 · Cited by 255
Transport of Quercetin and Its Glucosides across Human Intestinal Epithelial Caco-2 Cells
Walgren, R. A., Walle, U. K., & Walle, T. (1998) · Biochemical Pharmacology · doi:10.1016/s0006-2952(98)00048-3 · Cited by 249
Dietary flavonoids protect human colonocyte DNA from oxidative attack in vitro
Duthie, S. J., & Dobson, V. L. (1999) · European Journal of Nutrition · doi:10.1007/s003940050043 · Cited by 239
Duthie, S. J., Collins, A. R., Duthie, G. G., & Dobson, V. L. (1997) · Mutation Research/Genetic Toxicology and Environmental Mutagenesis · doi:10.1016/s1383-5718(97)00107-1 · Cited by 231
Bingham, S. A. (1990) · Proceedings of The Nutrition Society · doi:10.1079/pns19900021 · Cited by 204
Sestili, P., Guidarelli, A., Dachà, M., & Cantoni, O. (1998) · Free Radical Biology and Medicine · doi:10.1016/s0891-5849(98)00040-9 · Cited by 199
Dietary antioxidants and risk of myocardial infarction in the elderly: the Rotterdam Study
Klipstein‐Grobusch, K., Geleijnse, J. M., den Breeijen, J. H., Boeing, H., Albert Hofman, D., Grobbee, D. E., & Witteman, J. C. M. (1999) · American Journal of Clinical Nutrition · doi:10.1093/ajcn/69.2.261 · Cited by 198
Armstrong, B. K., Mann, J. I., Adelstein, A. M., & ESKIN, F. (1975) · Journal of Chronic Diseases · doi:10.1016/0021-9681(75)90056-9 · Cited by 185
Edenharder, R., von Petersdorff, I., & Rauscher, R. (1993) · Mutation research. Fundamental and molecular mechanisms of mutagenesis · doi:10.1016/0027-5107(93)90019-c · Cited by 174
Dietary Antioxidants and Lung Cancer Risk: A Case-Control Study in Uruguay
Stéfani, E. D., Boffetta, P., Deneo‐Pellegrini, H., Mendilaharsu, M., Carzoglio, J. C., Ronco, Á. L., & Olivera, L. (1999) · Nutrition and Cancer · doi:10.1207/s15327914nc340114 · Cited by 173
Duthie, S. J., Johnson, W., & Dobson, V. L. (1997) · Mutation Research/Genetic Toxicology and Environmental Mutagenesis · doi:10.1016/s0165-1218(97)00010-4 · Cited by 162
Molecular mechanisms in the antiproliferative action of quercetin
Csókay, B., Prajda, N., Weber, G., & Oláh, E. (1997) · Life Sciences · doi:10.1016/s0024-3205(97)00230-0 · Cited by 162
An Overview of the epidemiological evidence linking diet and cancer
Doll, R. (1990) · Proceedings of The Nutrition Society · doi:10.1079/pns19900018 · Cited by 149
Tsuda, T., Horio, F., Kitoh, J., & Osawa, T. (1999) · Archives of Biochemistry and Biophysics · doi:10.1006/abbi.1999.1311 · Cited by 145
Dietary cyanidin 3‐O‐β‐ d ‐glucoside increases ex vivo oxidation resistance of serum in rats
Tsuda, T., Horio, F., & Osawa, T. (1998) · Lipids · doi:10.1007/s11745-998-0243-5 · Cited by 143
Musonda, C. (1998) · Carcinogenesis · doi:10.1093/carcin/19.9.1583 · Cited by 141
McHale, J. F., Harari, O., Marshall, D. R., & Haskard, D. O. (1999) · PubMed · Cited by 125
Fate of the Flavonoid Quercetin in Human Cell Lines: Chemical Instability and Metabolism
Boulton, D. W., Walle, U. K., & Walle, T. (1999) · Journal of Pharmacy and Pharmacology · doi:10.1211/0022357991772367 · Cited by 125
Molecular Signals for Glial Activation: Pro- and Anti-Inflammatory Cytokines in the Injured Brain
Raivich, G., Jones, L. L., Werner, A., Blüthmann, H., Doetschmann, T., & Kreutzberg, G. W. (1999) · Acta neurochirurgica. Supplementum · doi:10.1007/978-3-7091-6391-7_4 · Cited by 123
McHale, J. F., Harari, O., Marshall, D., & Haskard, D. O. (1999) · The Journal of Immunology · doi:10.4049/jimmunol.163.7.3993 · Cited by 108
Manthey, J. A., Grohmann, K., Montanari, A. M., Ash, K., & Manthey, C. L. (1999) · Journal of Natural Products · doi:10.1021/np980431j · Cited by 99
Studies on Vaccinium myrtillus anthocyanosides. I. Vasoprotective and antiinflammatory activity.
Lietti, A., Cristoni, A., & Picci, M. (1976) · PubMed · Cited by 98
Increased expression of ICAM-1 during reoxygenation in brain endothelial cells.
Hess, D. C., Zhao, W., Carroll, J. E., McEachin, M., & Buchanan, K. (1994) · Stroke · doi:10.1161/01.str.25.7.1463 · Cited by 86
Antibacterial activity of flavonoids against Staphylococcus epidermidis, a skin bacterium.
Nishino, C., Enoki, N., Tawata, S., Akihisa, M. O. R. I., Kobayashi, K., & Fukushima, M. (1987) · Agricultural and Biological Chemistry · doi:10.1271/bbb1961.51.139 · Cited by 84
Agullo, G., Gamet‐Payrastre, L., Fernandez, Y., Anciaux, N., Demigné, C., & Rémésy, C. (1996) · Cancer Letters · doi:10.1016/0304-3835(96)04262-0 · Cited by 78
Modulation of intestinal functions by food substances
Shimizu, M. (1999) · Food / Nahrung · doi:10.1002/(sici)1521-3803(19990601)43:3 · Cited by 77
Panés, J., Gerritsen, M. E., Anderson, D. C., Miyasaka, M., & Granger, D. N. (1996) · Microcirculation · doi:10.3109/10739689609148302 · Cited by 76
Virgili, F., Kim, D., & Packer, L. (1998) · FEBS Letters · doi:10.1016/s0014-5793(98)00778-9 · Cited by 73
Howard, E. F., Chen, Q., Cheng, C. Y., Carroll, J. E., & Hess, D. C. (1998) · Neuroscience Letters · doi:10.1016/s0304-3940(98)00239-0 · Cited by 71
ICAM-1 Induction in the Mouse CNS Following Irradiation
Olschowka, J. A., Kyrkanides, S., Harvey, B. K., O’Banion, M. K., Williams, J. P., Rubin, P., & Hansen, J. T. (1997) · Brain Behavior and Immunity · doi:10.1006/brbi.1997.0506 · Cited by 70
β-Amyloid-Induced Endothelial Necrosis and Inhibition of Nitric Oxide Production
Sutton, E. T., Hellermann, G., & Thomas, T. (1997) · Experimental Cell Research · doi:10.1006/excr.1996.3440 · Cited by 66
Vaccinium myrtillus anthocyanosides pharmacokinetics in rats.
Morazzoni, P., Livio, S., Scilingo, A., & Malandrino, S. (1991) · PubMed · Cited by 61
KANEKO, T., & Baba, N. (1999) · Bioscience Biotechnology and Biochemistry · doi:10.1271/bbb.63.323 · Cited by 58
Potential effects of flavonoids on the etiology of vascular disease
Schramm, D. D., & German, J. B. (1998) · The Journal of Nutritional Biochemistry · doi:10.1016/s0955-2863(98)00051-5 · Cited by 58
Kimura, Y., Matsushita, N., & Okuda, H. (1997) · Journal of Ethnopharmacology · doi:10.1016/s0378-8741(97)00045-7 · Cited by 37
Studies on vascular permeability in hypertension: action of anthocyanosides.
Detre, Z., Jellinek, H., Miskulin, M., & Robert, A. M. (1986) · PubMed · Cited by 37
Noteborn, H. P. J. M., Jansen, E. J., Benito, S., & Mengelers, M. J. B. (1997) · Cancer Letters · doi:10.1016/s0304-3835(97)04655-7 · Cited by 35
Comparative protection against oxyradicals by three flavonoids on cultured endothelial cells
Zeng, L.-H., Wu, J., Fung, B., Tong, J., Mickle, D. A. G., & Wu, T.-W. (1997) · Biochemistry and Cell Biology · doi:10.1139/o97-062 · Cited by 31
Milne, M. L., Kang, M.-I., Cardona, G. R., Quail, J. M., Braverman, L. E., Chin, W. W., & Baran, D. T. (1999) · Journal of Cellular Biochemistry · doi:10.1002/(sici)1097-4644(19990915)74:4 · Cited by 29
Lenne-Gouverneur, A. A.-F. (1999) · Molecular Membrane Biology · doi:10.1080/096876899294616 · Cited by 27
Bioactivity studies ofSalvia transsylvanica (Schur ex Griseb) grown in Egypt
Maklad, Y. A., Aboutabl, E. A., El-Sherei, M. M., & Meselhy, K. M. (1999) · Phytotherapy Research · doi:10.1002/(sici)1099-1573(199903)13:2 · Cited by 21
Aortic endothelial cells damaged by a nitric oxide donor and protected by flavonoids
Law, A., Wu, J., Zeng, L.-H., & Wu, T.-W. (1999) · Life Sciences · doi:10.1016/s0024-3205(99)00118-6 · Cited by 18
Khaza’ai, H., & Wahle, K. W. J. (1996) · Biochemical Society Transactions · doi:10.1042/bst024173s · Cited by 7
4 Modulation of AAPH-induced oxidative stress in cell culture by flavonoids
Plumb, G. W., DuPont, M. S., & Williamson, G. (1997) · Biochemical Society Transactions · doi:10.1042/bst025s560 · Cited by 4
Research works printed but not linked: 2
- Detre, 1986, Studies on vascular permeability in hypertension, Clin. Physiol. Biochem., 4, 143
- Mosmann, 1983, Rapid colorimetric assay for cellular growth and survival, J. Immunol., 65, 55
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Analysis and biological activities of anthocyanins
Kong, J.-M., Chia, L. S., Goh, N.-K., Chia, T., & Brouillard, R. (2003) · Phytochemistry · doi:10.1016/s0031-9422(03)00438-2 · Cited by 1,689
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Metadata
- Authors
- Youdim, K. A., Martin, A., & Joseph, J. A.
- Affiliations
- Tufts University (US)
- Corresponding author
- Youdim, Kuresh A
- Venue
- Free Radical Biology and Medicine
- Publisher
- Elsevier BV
- Published
- 2000-07
- Volume
- 29
- Issue
- 1
- Pages
- 51-60
- ISSN
- 0891-5849 (ISSN-L)
- OpenAlex
- W2154801407
- Cited by
- 312
- References
- 65
- Citations by year
- 2026: 3 · 2025: 4 · 2024: 7 · 2023: 10 · 2022: 13 · 2021: 5 · 2020: 6 · 2019: 10 · 2018: 11 · 2017: 20 · 2016: 10 · 2015: 18 · 2014: 12 · 2013: 22 · 2012: 17
- FWCI
- 5.45
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Free Radical Biology and Medicine (publishedVersion)
- PubMed (publishedVersion)
- Funders
- U.S. Department of Agriculture
- Topics
- Health Sciences › Medicine › Biochemistry (medical) › Phytochemicals and Antioxidant Activities
- Health Sciences › Medicine › Physiology (medical) › Nitric Oxide and Endothelin Effects
- Health Sciences › Medicine › Biochemistry (medical) › Antioxidant Activity and Oxidative Stress
- Keywords
- endothelial cells, oxidative stress, endothelial dysfunction, diglucosides, hydrogen peroxide, AAPH, iron ascorbate, antioxidant defense, vascular disease
- MeSH terms
- Amidines, Animals, Anthocyanins, Aorta, Ascorbic Acid, Cattle, Cell Division, Cells, Cultured, Endothelium, Vascular, Ferric Compounds, Fruit, Humans, Kinetics, Mitochondria, Plant Extracts, Oxidants, Oxidative Stress
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