Activity Regulates the Synaptic Localization of the NMDA Receptor in Hippocampal Neurons
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We describe here a novel effect of activity on the subcellular distribution of NMDA receptors in hippocampal neurons in culture. In spontaneously active neurons, NMDA receptors were clustered at a few synaptic and nonsynaptic sites. Chronic blockade of NMDA receptor activity induced a 380% increase in the number of NMDA receptor clusters and a shift to a more synaptic distribution. This effect was reversible. The distributions of the presynaptic marker synaptophysin, the AMPA-type glutamate receptor subunit GluR1, and the putative NMDA receptor clustering protein PSD-95 were not affected by blockade. Regulation of the synaptic localization of NMDA receptors by activity may define a novel mechanism by which input controls a neuron's ability to modify its synapses.
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Actin filaments participate in membrane targeting of NMDA receptor clusters, an effect regulated by activity at NMDA receptors themselves.
“Actin filaments participate in membrane targeting of NMDA receptor clusters (Allison et al., 1998), an effect that is regulated by activity at NMDA receptors themselves (Rao and Craig, 1997).”
From Regulation of F-Actin Stability in Dendritic Spines by Glutamate Receptors and Calcineurin (1998) · cites this paper as Rao and Craig, 1997
NMDARs are typically arranged in clusters at synaptic and extrasynaptic sites.
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- Authors
- Rao, A., & Craig, A. M.
- Affiliations
- University of Illinois Urbana-Champaign (US)
- Corresponding author
- Craig, Ann Marie
- ORCID
- Craig, Ann Marie
- Venue
- Neuron
- Publisher
- Elsevier BV
- Published
- 1997-10
- Volume
- 19
- Issue
- 4
- Pages
- 801-812
- ISSN
- 0896-6273 (ISSN-L)
- OpenAlex
- W2037921808
- Cited by
- 492
- References
- 3
- Citations by year
- 2026: 3 · 2025: 3 · 2024: 3 · 2023: 7 · 2022: 5 · 2021: 6 · 2020: 5 · 2019: 8 · 2018: 4 · 2017: 6 · 2016: 8 · 2015: 11 · 2014: 13 · 2013: 9 · 2012: 16
- FWCI
- 12.72
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Neuron (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of Neurological Disorders and Stroke (NS33184, R01NS033184)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Amino Acid Enzymes and Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetics and Neurodevelopmental Disorders
- Keywords
- NMDA receptor, synaptic localization, hippocampal neurons, neuronal activity, receptor clustering, NMDA receptor blockade, synaptophysin, GluR1, PSD-95, synaptic plasticity, subcellular localization
- MeSH terms
- Disks Large Homolog 4 Protein, Animals, Cells, Cultured, Embryo, Mammalian, Hippocampus, Membrane Proteins, Nerve Tissue Proteins, Neurons, Synapses, Tetrodotoxin, 2-Amino-5-phosphonovalerate, Up-Regulation, Receptors, N-Methyl-D-Aspartate, Synaptophysin, Receptors, AMPA, Intracellular Signaling Peptides and Proteins, Rats
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