Cited by 492Neuroscience and Neuropharmacology ResearchAmino Acid Enzymes and MetabolismGenetics and Neurodevelopmental Disorders

Activity Regulates the Synaptic Localization of the NMDA Receptor in Hippocampal Neurons

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Rao, Anuradha, Craig, Ann Marie·1997·Neuron

Abstract

We describe here a novel effect of activity on the subcellular distribution of NMDA receptors in hippocampal neurons in culture. In spontaneously active neurons, NMDA receptors were clustered at a few synaptic and nonsynaptic sites. Chronic blockade of NMDA receptor activity induced a 380% increase in the number of NMDA receptor clusters and a shift to a more synaptic distribution. This effect was reversible. The distributions of the presynaptic marker synaptophysin, the AMPA-type glutamate receptor subunit GluR1, and the putative NMDA receptor clustering protein PSD-95 were not affected by blockade. Regulation of the synaptic localization of NMDA receptors by activity may define a novel mechanism by which input controls a neuron's ability to modify its synapses.

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Crossref · 10.1016/s0896-6273(00)80962-9

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Authors
Rao, A., & Craig, A. M.
Affiliations
University of Illinois Urbana-Champaign (US)
Corresponding author
Craig, Ann Marie
Venue
Neuron
Publisher
Elsevier BV
Published
1997-10
Volume
19
Issue
4
Pages
801-812
ISSN
0896-6273 (ISSN-L)
OpenAlex
W2037921808
Cited by
492
References
3
Citations by year
2026: 3 · 2025: 3 · 2024: 3 · 2023: 7 · 2022: 5 · 2021: 6 · 2020: 5 · 2019: 8 · 2018: 4 · 2017: 6 · 2016: 8 · 2015: 11 · 2014: 13 · 2013: 9 · 2012: 16
FWCI
12.72
Type
journal article
Language
en
Open access
Locations
  • Neuron (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Institute of Neurological Disorders and Stroke (NS33184, R01NS033184)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Amino Acid Enzymes and Metabolism
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetics and Neurodevelopmental Disorders
Keywords
NMDA receptor, synaptic localization, hippocampal neurons, neuronal activity, receptor clustering, NMDA receptor blockade, synaptophysin, GluR1, PSD-95, synaptic plasticity, subcellular localization
MeSH terms
Disks Large Homolog 4 Protein, Animals, Cells, Cultured, Embryo, Mammalian, Hippocampus, Membrane Proteins, Nerve Tissue Proteins, Neurons, Synapses, Tetrodotoxin, 2-Amino-5-phosphonovalerate, Up-Regulation, Receptors, N-Methyl-D-Aspartate, Synaptophysin, Receptors, AMPA, Intracellular Signaling Peptides and Proteins, Rats

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