Cloning and expression of a farnesyl diphosphate synthase in Centella asiatica (L.) Urban

Kim, Ok Tae; Ahn, Jun Cheul; Hwang, Sung Jin; Hwang, Baik

doi:10.1016/s1016-8478(23)13171-2

Cited by 29Plant biochemistry and biosynthesisPlant Gene Expression AnalysisPlant tissue culture and regeneration

Cloning and expression of a farnesyl diphosphate synthase in Centella asiatica (L.) Urban

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Kim, Ok Tae, Ahn, Jun Cheul, Hwang, Sung Jin, Hwang, Baik·2005·Molecules and Cells

DOI 10.1016/s1016-8478(23)13171-2

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Abstract

A cDNA encoding farnesyl diphosphate synthase (FPS; EC2.5.1.1/EC2.5.1.10) was isolated from Centella asiacita (L.) Urban, using degenerate primers based on two highly conserved domains. A full-length cDNA clone was subsequently isolated by rapid amplification of cDNA ends (RACE) PCR. The sequence of the CaFPS (C. asiatica farnesyl diphosphate synthase) cDNA contains an open reading frame of 1029 nucleotides encoding 343 amino acids with a molecular mass of 39.6 kDa. The deduced CaFPS amino acid sequence exhibits 84, 79, and 72%, identity to the FPSs of Artemisia annua, Arabidopsis thaliana, and Oryza sativa, respectively. Southern blot analysis suggested that the C. asiatica genome contains only one FPS gene. An artificially expressed soluble form of the CaFPS was identified by SDS-PAGE. It had high specific activity and produced farnesyl diphosphate as the major isoprenoid.

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Investigators have concentrated on expressing enzymes from phases 1 and 2 of the artemisinin biosynthetic route in transgenic organisms, including HMGR, FPPS, ADS, and CYP71AV1.
“To date, investigators have concentrated on expressing the enzymes from phases 1 and 2 of the biosynthetic route to artemisinin in transgenic organisms (see Scheme 9 in Section 3). These have included: HMG-CoA reductase (HMGR) [370]; farnesyl diphosphate synthase (FPPS) [371, 372,373,374]; amorpha-4,11-diene synthase (ADS) [375,376,377]; and the P450 enzyme which oxidises amorpha-4,11-diene to art”
From The Biosynthesis of Artemisinin (Qinghaosu) and the Phytochemistry of Artemisia annua L. (Qinghao) (2010) · cites this paper as [370]

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Authors: Kim, O. T., Ahn, J. C., Hwang, S. J., & Hwang, B.
Affiliations: Chonnam National University (KR)
Seonam University (KR)
Dongshin University (KR)
Corresponding author: Hwang, Baik
ORCID: Kim, Ok Tae
Ahn, Jun Cheul
Hwang, Sung Jin
Venue: Molecules and Cells
Publisher: Elsevier BV
Published: 2005-04
Volume: 19
Issue: 2
Pages: 294-299
ISSN: 0219-1032 (ISSN-L)
1016-8478
DOI: 10.1016/s1016-8478(23)13171-2
OpenAlex: W2419485035
Cited by: 29
References: 30
Citations by year: 2025: 4 · 2024: 1 · 2023: 1 · 2022: 1 · 2021: 2 · 2019: 1 · 2018: 1 · 2017: 4 · 2016: 1 · 2014: 2 · 2013: 3
FWCI: 0.38
Type: journal article
Language: en
Open access: hybrid
doi.org
Licence: cc-by-nc-sa
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
www.elsevier.com/legal/tdmrep-license (tdm)
creativecommons.org/licenses/by-nc-sa/3.0/ (vor, 29 d)
Locations: Molecules and Cells (publishedVersion, cc-by-nc-sa)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant Gene Expression Analysis
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
Keywords: farnesyl diphosphate synthase, Centella asiatica, cDNA cloning, gene expression, isoprenoid biosynthesis, protein purification, sequence homology, single-copy gene
MeSH terms: Amino Acid Sequence, Base Sequence, Cloning, Molecular, Escherichia coli, Molecular Sequence Data, Polyisoprenyl Phosphates, Recombinant Proteins, Sesquiterpenes, Blotting, Southern, Open Reading Frames, Sequence Homology, Amino Acid, DNA, Complementary, Alkyl and Aryl Transferases, Centella, Geranyltranstransferase

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Cloning and expression of a farnesyl diphosphate synthase in Centella asiatica (L.) Urban

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