The DOI matches its Crossref record; 9 of the 9 references it cites were found in a registry or an index.
Single-Cell Microarray for Analyzing Cellular Response
Abstract
Detection of cellular response by measuring intracellular calcium, (Ca2+)i with Ca2+-dependent fluorescent dye are standard approaches to detect ligand-stimulated cells and to study signaling through ligand/receptor interaction. We describe a single-cell microarray system to analyze cellular response of individual cells such as lymphocytes using microchamber array chips. The single-cell microarray chip is made from polystyrene with over 30,000 microchambers, which can accommodate only single cells. Lymphocytes derived from mouse spleen or human blood were spread on the microarray, and over 80% of the microchambers achieved single-cell status. Stimulation of B-cells through antigen receptors on the microarray allowed us to detect activated B-cells by comparing the states of single B-cells before and after stimulation with antigen, which is disabled for flow cytometry. In addition, this novel method demonstrated retrieval of positive single B-cells from microchambers by a micromanipulator and achieved antibody DNA analysis. The system is suitable for high-throughput analysis of intracellular Ca2+ response at the single-cell level and is applicable to screen antigen-specific lymphocytes for making specific monoclonal antibody.
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Cites
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Microfluidic Device for Single-Cell Analysis
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High efficiency creation of human monoclonal antibody-producing hybridomas
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Cited by
A trap-and-release integrated microfluidic system for dynamic microarray applications
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High-throughput sequencing of the paired human immunoglobulin heavy and light chain repertoire
DeKosky, B. J., Ippolito, G. C., Deschner, R. P., Lavinder, J. J., Wine, Y., Rawlings, B. M., Varadarajan, N., Giesecke, C., Dörner, T., Andrews, S. F., et al. (2013) · Nature Biotechnology · doi:10.1038/nbt.2492 · Cited by 410
Single Cell Optical Imaging and Spectroscopy
Stender, A. S., Marchuk, K., Liu, C., Sander, S., Meyer, M. W., Smith, E. A., Neupane, B., Wang, G., Li, J., Cheng, J.-X., et al. (2013) · Chemical Reviews · doi:10.1021/cr300336e · Cited by 292
Ingham, C. J., Sprenkels, A., Bomer, J., Molenaar, D., van den Berg, A., van Hylckama Vlieg, J. E. T., & de Vos, W. M. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0701693104 · Cited by 280
Jin, A., Ozawa, T., Tajiri, K., Obata, T., Kondo, S., Kinoshita, K., Kadowaki, S., Takahashi, K., Sugiyama, T., Kishi, H., et al. (2009) · Nature Medicine · doi:10.1038/nm.1966 · Cited by 261
Self-citation
Metadata
- Authors
- Yamamura, S., Kishi, H., Tokimitsu, Y., Kondo, S., Honda, R., Rao, S. R., Omori, M., Tamiya, E., & Muraguchi, A.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2005-11-12
- Volume
- 77
- Issue
- 24
- Pages
- 8050-8056
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W1982897852
- Cited by
- 258
- References
- 9
- FWCI
- 5.19
- Type
- journal article
- Language
- en
- Open access
- closed
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- Yamamura, S., Kishi, H., Tokimitsu, Y., Kondo, S., Honda, R., Rao, S. R., Omori, M., Tamiya, E., & Muraguchi, A. (2005). Single-Cell Microarray for Analyzing Cellular Response. Analytical Chemistry. https://doi.org/10.1021/ac0515632
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