The DOI matches its Crossref record; 23 of the 23 references it cites were found in a registry or an index.
Selective Zirconium Dioxide-Based Enrichment of Phosphorylated Peptides for Mass Spectrometric Analysis
Abstract
Due to the dynamic nature and low stoichiometry of protein phosphorylation, enrichment of phosphorylated peptides from proteolytic mixtures is often necessary prior to their characterization by mass spectrometry. Several phosphopeptide isolation strategies have been presented in the literature, including immobilized metal ion affinity chromatography. However, that technique suffers from poor selectivity and reproducibility. Recently, titanium dioxide-based columns have been successfully employed for phosphopeptide enrichment by several research groups. Here, we present, to our knowledge, the first demonstration of the utility of zirconium dioxide microtips for phosphopeptide isolation prior to mass spectrometric analysis. These microtips display similar overall performance as TiO2 microtips. However, more selective isolation of singly phosphorylated peptides was observed with ZrO2 compared to TiO2 whereas TiO2 preferentially enriched multiply phosphorylated peptides. Thus, these two chromatographic materials possess complementary properties. For alpha- and beta-casein, Glu-C digestion provided no evident advantage compared to trypsin digestion when combined with TiO2 or ZrO2 phosphopeptide enrichment.
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Cites
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Analysis of protein phosphorylation using mass spectrometry: deciphering the phosphoproteome
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Immobilized Gallium(III) Affinity Chromatography of Phosphopeptides
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Proceedings of the second international conference on the peaceful uses of atomic energy
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Phosphoproteome Analysis of Capacitated Human Sperm
Ficarro, S., Chertihin, O., Westbrook, V. A., White, F., Jayes, F., Kalab, P., Marto, J. A., Shabanowitz, J., Herr, J. C., Hunt, D. F., et al. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m202325200 · Cited by 480
Chemistry of zirconia and its use in chromatography
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Cited by
Protein Analysis by Shotgun/Bottom-up Proteomics
Zhang, Y., Fonslow, B. R., Shan, B., Baek, M.-C., & Yates, J. R. (2013) · Chemical Reviews · doi:10.1021/cr3003533 · Cited by 1,491
Sugiyama, N., Masuda, T., Shinoda, K., Nakamura, A., Tomita, M., & Ishihama, Y. (2007) · Molecular & Cellular Proteomics · doi:10.1074/mcp.t600060-mcp200 · Cited by 435
Zhao, Y., & Jensen, O. N. (2009) · PROTEOMICS · doi:10.1002/pmic.200900398 · Cited by 384
Jensen, S. S., & Larsen, M. R. (2007) · Rapid Communications in Mass Spectrometry · doi:10.1002/rcm.3254 · Cited by 274
Catch me if you can: Mass spectrometry‐based phosphoproteomics and quantification strategies
Eyrich, B., Sickmann, A., & Zahedi, R. P. (2011) · PROTEOMICS · doi:10.1002/pmic.201000489 · Cited by 104
Metadata
- Authors
- Kweon, H. K., & Håkansson, K.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2006-02-07
- Volume
- 78
- Issue
- 6
- Pages
- 1743-1749
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2108136469
- Cited by
- 489
- References
- 23
- FWCI
- 22.19
- Type
- journal article
- Language
- en
- Open access
- closed
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