The DOI matches its Crossref record; 23 of the 23 references it cites were found in a registry or an index.
Selective Zirconium Dioxide-Based Enrichment of Phosphorylated Peptides for Mass Spectrometric Analysis
Abstract
Due to the dynamic nature and low stoichiometry of protein phosphorylation, enrichment of phosphorylated peptides from proteolytic mixtures is often necessary prior to their characterization by mass spectrometry. Several phosphopeptide isolation strategies have been presented in the literature, including immobilized metal ion affinity chromatography. However, that technique suffers from poor selectivity and reproducibility. Recently, titanium dioxide-based columns have been successfully employed for phosphopeptide enrichment by several research groups. Here, we present, to our knowledge, the first demonstration of the utility of zirconium dioxide microtips for phosphopeptide isolation prior to mass spectrometric analysis. These microtips display similar overall performance as TiO2 microtips. However, more selective isolation of singly phosphorylated peptides was observed with ZrO2 compared to TiO2 whereas TiO2 preferentially enriched multiply phosphorylated peptides. Thus, these two chromatographic materials possess complementary properties. For alpha- and beta-casein, Glu-C digestion provided no evident advantage compared to trypsin digestion when combined with TiO2 or ZrO2 phosphopeptide enrichment.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Hunter, T. (2000) · Cell · doi:10.1016/s0092-8674(00)81688-8 · Cited by 2,586
Analysis of protein phosphorylation using mass spectrometry: deciphering the phosphoproteome
Mann, M., Ong, S.-E., Grønborg, M., Steen, H., Jensen, O. N., & Pandey, A. (2002) · Trends in Biotechnology · doi:10.1016/s0167-7799(02)01944-3 · Cited by 900
Pinkse, M. W. H., Uitto, P. M., Hilhorst, M. J., Ooms, B., & Heck, A. J. R. (2004) · Analytical Chemistry · doi:10.1021/ac0498617 · Cited by 891
Immobilized Gallium(III) Affinity Chromatography of Phosphopeptides
Posewitz, M. C., & Tempst, P. (1999) · Analytical Chemistry · doi:10.1021/ac981409y · Cited by 832
Proceedings of the second international conference on the peaceful uses of atomic energy
Smith, D. B. (1960) · The International Journal of Applied Radiation and Isotopes · doi:10.1016/0020-708x(60)90029-6 · Cited by 705
Nühse, T. S., Stensballe, A., Jensen, O. N., & Peck, S. C. (2003) · Molecular & Cellular Proteomics · doi:10.1074/mcp.t300006-mcp200 · Cited by 545
Phosphoproteome Analysis of Capacitated Human Sperm
Ficarro, S., Chertihin, O., Westbrook, V. A., White, F., Jayes, F., Kalab, P., Marto, J. A., Shabanowitz, J., Herr, J. C., Hunt, D. F., et al. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m202325200 · Cited by 480
Chemistry of zirconia and its use in chromatography
Nawrocki, J., Rigney, M., McCormick, A., & Carr, P. W. (1993) · Journal of Chromatography A · doi:10.1016/0021-9673(93)80284-f · Cited by 419
Pandey, A., Podtelejnikov, A. V., Blagoev, B., Bustelo, X. R., Mann, M., & Lodish, H. F. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.97.1.179 · Cited by 406
Analysis of phosphorylated proteins and peptides by mass spectrometry
McLachlin, D. T., & Chait, B. T. (2001) · Current Opinion in Chemical Biology · doi:10.1016/s1367-5931(00)00250-7 · Cited by 382
Electron capture dissociation of singly and multiply phosphorylated peptides
Stensballe, A., Jensen, O. N., Olsen, J. V., Haselmann, K. F., & Zubarev, R. A. (2000) · Rapid Communications in Mass Spectrometry · doi:10.1002/1097-0231(20001015)14:19<1793::aid-rcm95>3.0.co;2-q · Cited by 348
Wolschin, F., Wienkoop, S., & Weckwerth, W. (2005) · PROTEOMICS · doi:10.1002/pmic.200402049 · Cited by 296
Neville, D. C. A., Townsend, R. R., Rozanas, C. R., Verkman, A. S., Price, E. M., & Gruis, D. B. (1997) · Protein Science · doi:10.1002/pro.5560061117 · Cited by 247
Grün, M., Kurganov, A. A., Schacht, S., Schüth, F., & Unger, K. K. (1996) · Journal of Chromatography A · doi:10.1016/0021-9673(96)00205-1 · Cited by 246
Improved β-Elimination-Based Affinity Purification Strategy for Enrichment of Phosphopeptides
McLachlin, D. T., & Chait, B. T. (2003) · Analytical Chemistry · doi:10.1021/ac034989u · Cited by 225
Peer Reviewed: Calixarenes: Designer Ligands for Chemical Sensors
Diamond, D., & Nolan, K. (2001) · Analytical Chemistry · doi:10.1021/ac012376g · Cited by 172
Haydon, C. E., Eyers, P. A., Aveline-Wolf, L. D., Resing, K. A., Maller, J. L., & Ahn, N. G. (2003) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m300054-mcp200 · Cited by 137
Analysis of protein phosphorylation by mass spectrometry
Garcia, B. A., Shabanowitz, J., & Hunt, D. F. (2005) · Methods · doi:10.1016/j.ymeth.2004.08.017 · Cited by 136
Detection and localization of protein modifications by high resolution tandem mass spectrometry
Meng, F., Forbes, A. J., Miller, L. M., & Kelleher, N. L. (2004) · Mass Spectrometry Reviews · doi:10.1002/mas.20009 · Cited by 133
The Characterization of Protein Post-Translational Modifications by Mass Spectrometry
Schweppe, R. E., Haydon, C. E., Lewis, T. S., Resing, K. A., & Ahn, N. G. (2003) · Accounts of Chemical Research · doi:10.1021/ar020143l · Cited by 92
Strategies for shotgun identification of post-translational modifications by mass spectrometry
Cantin, G. T., & YATESIII, J. (2004) · Journal of Chromatography A · doi:10.1016/s0021-9673(04)00971-9 · Cited by 84
Chalmers, M. J., Håkansson, K., Johnson, R. W., Smith, R. A., Shen, J., Emmett, M. R., & Marshall, A. G. (2004) · PROTEOMICS · doi:10.1002/pmic.200300650 · Cited by 81
Self-citationIdentification and analysis of phosphopeptides
Chalmers, M. J., Kölch, W., Emmett, M. R., et al. (2003) · Journal of Chromatography B · doi:10.1016/j.jchromb.2003.09.006 · Cited by 70
Cited by
Protein Analysis by Shotgun/Bottom-up Proteomics
Zhang, Y., Fonslow, B. R., Shan, B., Baek, M.-C., & Yates, J. R. (2013) · Chemical Reviews · doi:10.1021/cr3003533 · Cited by 1,491
Sugiyama, N., Masuda, T., Shinoda, K., Nakamura, A., Tomita, M., & Ishihama, Y. (2007) · Molecular & Cellular Proteomics · doi:10.1074/mcp.t600060-mcp200 · Cited by 435
Zhao, Y., & Jensen, O. N. (2009) · PROTEOMICS · doi:10.1002/pmic.200900398 · Cited by 384
Jensen, S. S., & Larsen, M. R. (2007) · Rapid Communications in Mass Spectrometry · doi:10.1002/rcm.3254 · Cited by 274
Catch me if you can: Mass spectrometry‐based phosphoproteomics and quantification strategies
Eyrich, B., Sickmann, A., & Zahedi, R. P. (2011) · PROTEOMICS · doi:10.1002/pmic.201000489 · Cited by 104
Metadata
- Authors
- Kweon, H. K., & Håkansson, K.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2006-02-07
- Volume
- 78
- Issue
- 6
- Pages
- 1743-1749
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2108136469
- Cited by
- 489
- References
- 23
- FWCI
- 22.19
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
