In Vivo Live Cell Imaging for the Quantitative Monitoring of Lipids by Using Raman Microspectroscopy
Abstract
A straightforward in vivo monitoring technique for biomolecules would be an advantageous approach for understanding their spatiotemporal dynamics in living cells. However, the lack of adequate probes has hampered the quantitative determination of the chemical composition and metabolomics of cellular lipids at single-cell resolution. Here, we describe a method for the rapid, direct, and quantitative determination of lipid molecules from living cells using single-cell Raman imaging. In vivo localization of lipids in the form of triacylglycerol (TAG) within oleaginous microalga and their molecular compositions are monitored with high spatial resolution in a nondestructive and label-free manner. This method can provide quantitative and real-time information on compositions, chain lengths, and degree of unsaturation of fatty acids in living cells for improving the cultivating parameters or for determining the harvest timing during large-scale cultivations for microalgal lipid accumulation toward biodiesel production. Therefore, this technique is a potential tool for in vivo lipidomics for understanding the dynamics of lipid metabolisms in various organisms.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The z-resolution in CARS microscopy is in the range of 650 nm compared to 2000 nm for Raman microscopy.
“Furthermore, the z-resolution in CARS microscopy is in the range of 650 nm compared to 2000 nm18 for Raman microscopy”
From Label-free in vivo analysis of intracellular lipid droplets in the oleaginous microalga Monoraphidium neglectum by coherent Raman scattering microscopy (2016) · cites this paper as [18]
Cites
Guillard, R. R., & Ryther, J. (1962) · Canadian Journal of Microbiology (print) · doi:10.1139/m62-029 · Cited by 7,663 · see citing works
Stochastic Gene Expression in a Single Cell
Elowitz, M. B., Levine, A. J., Siggia, E. D., & Swain, P. S. (2002) · Science · doi:10.1126/science.1070919 · Cited by 5,441 · see citing works
Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances
Hu, Q., Sommerfeld, M., Jarvis, E., Ghirardi, M., Posewitz, M., Seibert, M., & Darzins, A. (2008) · The Plant Journal · doi:10.1111/j.1365-313x.2008.03492.x · Cited by 3,745 · see citing works
Rodolfi, L., Chini Zittelli, G., Bassi, N., Padovani, G., Biondi, N., Bonini, G., & Tredici, M. R. (2009) · Biotechnology and Bioengineering · doi:10.1002/bit.22033 · Cited by 2,879 · see citing works
Second Generation Biofuels: High-Efficiency Microalgae for Biodiesel Production
Schenk, P. M., Thomas‐Hall, S. R., Stephens, E., Marx, U. C., Mussgnug, J. H., Posten, C. H., Kruse, O., & Hankamer, B. D. (2008) · BioEnergy Research · doi:10.1007/s12155-008-9008-8 · Cited by 2,335 · see citing works
Creating new fluorescent probes for cell biology
Zhang, J., Campbell, R. E., Ting, A. Y., & Tsien, R. Y. (2002) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm976 · Cited by 1,984 · see citing works
Extraction of oil from microalgae for biodiesel production: A review
Halim, R., Danquah, M. K., & Webley, P. A. (2012) · Biotechnology Advances · doi:10.1016/j.biotechadv.2012.01.001 · Cited by 1,043 · see citing works
The biogenesis and functions of lipid bodies in animals, plants and microorganisms
Murphy, D. J. (2001) · Progress in Lipid Research · doi:10.1016/s0163-7827(01)00013-3 · Cited by 966 · see citing works
Davey, H. M., & Kell, D. B. (1996) · Microbiological Reviews · doi:10.1128/mr.60.4.641-696.1996 · Cited by 879 · see citing works
Analyzing biodiesel: standards and other methods
Knothe, G. (2006) · Journal of the American Oil Chemists Society · doi:10.1007/s11746-006-5033-y · Cited by 873 · see citing works
Plant triacylglycerols as feedstocks for the production of biofuels
Durrett, T. P., Christoph Benning, A., & Ohlrogge, J. B. (2008) · The Plant Journal · doi:10.1111/j.1365-313x.2008.03442.x · Cited by 697 · see citing works
Breuer, G., Lamers, P. P., Martens, D. E., Draaisma, R. B., & Wijffels, R. H. (2012) · Bioresource Technology · doi:10.1016/j.biortech.2012.08.003 · Cited by 626 · see citing works
Analysis of bacterial function by multi-colour fluorescence flow cytometry and single cell sorting
Nebe‐von‐Caron, G., Stephens, P., Hewitt, C. J., Powell, J. R., & BADLEY, R. A. (2000) · Journal of Microbiological Methods · doi:10.1016/s0167-7012(00)00181-0 · Cited by 455 · see citing works
In vivo lipidomics using single-cell Raman spectroscopy
Wu, H., Volponi, J. V., Oliver, A. E., Parikh, A. N., Simmons, B. A., & Singh, S. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1009043108 · Cited by 436 · see citing works
Use of fluorescent probes to assess physiological functions of bacteriaat single-cell level
Joux, F., & Lebaron, P. (2000) · Microbes and Infection · doi:10.1016/s1286-4579(00)01307-1 · Cited by 367 · see citing works
Fan, J., Andre, C., & Xu, C. (2011) · FEBS Letters · doi:10.1016/j.febslet.2011.05.018 · Cited by 347 · see citing works
The ecology of algal biodiesel production
Smith, V. H., Sturm, B. S. M., deNoyelles, F. J., & Billings, S. A. (2010) · Trends in Ecology & Evolution · doi:10.1016/j.tree.2009.11.007 · Cited by 277 · see citing works
Raman and CARS microspectroscopy of cells and tissues
Krafft, C., Dietzek, B., & Popp, J. (2009) · The Analyst · doi:10.1039/b822354h · Cited by 270 · see citing works
Fluorescent measurement of microalgal neutral lipids
Elsey, D., Jameson, D., Raleigh, B., & Cooney, M. J. (2007) · Journal of Microbiological Methods · doi:10.1016/j.mimet.2006.11.008 · Cited by 262 · see citing works
Goodson, C. M., Roth, R. A., Wang, Z. T., & Goodenough, U. W. (2011) · Eukaryotic Cell · doi:10.1128/ec.05242-11 · Cited by 252 · see citing works
Isolating lipid droplets from multiple species
Ding, Y., Zhang, S., Li, Y., Na, H., Zhang, P., Zhang, H., Wang, Y., Chen, Y., Yu, J., Huo, C., et al. (2012) · Nature Protocols · doi:10.1038/nprot.2012.142 · Cited by 220 · see citing works
Visualizing "green oil" in live algal cells
Cooper, M. S., Hardin, W. R., Petersen, T. W., & Cattolico, R. A. (2010) · Journal of Bioscience and Bioengineering · doi:10.1016/j.jbiosc.2009.08.004 · Cited by 215 · see citing works
Huang, Y.-S., Karashima, T., Yamamoto, M. P., & Hamaguchi, H. (2005) · Biochemistry · doi:10.1021/bi050179w · Cited by 209 · see citing works
Self-citationExtraction of hydrocarbons from microalga Botryococcus braunii with switchable solvents
Samorì, C., Torri, C., Samorì, G., Fabbri, D., Galletti, P., Guerrini, F., Pistocchi, R., & Tagliavini, E. (2010) · Bioresource Technology · doi:10.1016/j.biortech.2009.12.068 · Cited by 198 · see citing works
Raman microspectroscopy of individual algal cells: Sensing unsaturation of storage lipids in vivo
Samek, O., Jonáš, A., Pilát, Z., Zemánek, P., Nedbal, L., Tříska, J., Kotas, P., & Trtílek, M. (2010) · Sensors · doi:10.3390/s100908635 · Cited by 172 · see citing works
Quantitative analysis of oils and fats by Fourier transform Raman spectroscopy
Sadeghi‐Jorabchi, H., Wilson, R. H., Belton, P. S., Edwards‐Webb, J. D., & Coxon, D. T. (1991) · Spectrochimica Acta Part A Molecular Spectroscopy · doi:10.1016/0584-8539(91)80236-c · Cited by 153 · see citing works
Rapid screening method for lipid production in alga based on Nile red fluorescence
Huang, G.-H., Chen, G., & Chen, F. (2009) · Biomass and Bioenergy · doi:10.1016/j.biombioe.2009.05.022 · Cited by 147 · see citing works
Micro‐Raman spectroscopy of algae: Composition analysis and fluorescence background behavior
Huang, Y. Y., Beal, C. M., Cai, W. W., Ruoff, R. S., & Terentjev, E. M. (2010) · Biotechnology and Bioengineering · doi:10.1002/bit.22617 · Cited by 145 · see citing works
Molecular‐level pursuit of yeast mitosis by time‐ and space‐resolved Raman spectroscopy
Huang, Y., Karashima, T., Yamamoto, M. P., & Hamaguchi, H. (2002) · Journal of Raman Spectroscopy · doi:10.1002/jrs.960 · Cited by 89 · see citing works
Self-citationMatsumoto, M., Sugiyama, H., Maeda, Y., Sato, R., Tanaka, T., & Matsunaga, T. (2009) · Applied Biochemistry and Biotechnology · doi:10.1007/s12010-009-8766-x · Cited by 86 · see citing works
Self-citationHuang, C., Ando, M., Hamaguchi, H., & Shigeto, S. (2012) · Analytical Chemistry · doi:10.1021/ac300834f · Cited by 84 · see citing works
Self-citationWeiss, T. L., Chun, H. J., Okada, S., Vitha, S., Holzenburg, A., Laane, J., & Devarenne, T. P. (2010) · Journal of Biological Chemistry · doi:10.1074/jbc.m110.157230 · Cited by 83 · see citing works
Muto, M., Fukuda, Y., Nemoto, M., Yoshino, T., Matsunaga, T., & Tanaka, T. (2012) · Marine Biotechnology · doi:10.1007/s10126-012-9457-0 · Cited by 82 · see citing works
Self-citationHosokawa, M., Arakaki, A., Takahashi, M., Mori, T., Takeyama, H., & Matsunaga, T. (2009) · Analytical Chemistry · doi:10.1021/ac900535h · Cited by 75 · see citing works
Self-citationProteomics Analysis of Oil Body-Associated Proteins in the Oleaginous Diatom
Nojima, D., Yoshino, T., Maeda, Y., Tanaka, M., Nemoto, M., & Tanaka, T. (2013) · Journal of Proteome Research · doi:10.1021/pr4004085 · Cited by 66 · see citing works
Self-citationHuang, C., Hamaguchi, H., & Shigeto, S. (2011) · Chemical Communications · doi:10.1039/c1cc12350e · Cited by 55 · see citing works
Self-citationSatoh, A., Ichii, K., Matsumoto, M., Kubota, C., Nemoto, M., Tanaka, M., Yoshino, T., Matsunaga, T., & Tanaka, T. (2013) · Bioresource Technology · doi:10.1016/j.biortech.2013.03.087 · Cited by 47 · see citing works
Self-citationCharacterization of oil-producing microalgae using Raman spectroscopy
Samek, O., Zemánek, P., Jonáš, A., & Telle, H. H. (2011) · Laser Physics Letters · doi:10.1002/lapl.201110060 · Cited by 47 · see citing works
Raman microspectroscopy of algal lipid bodies: β-carotene quantification
Pilát, Z., Bernatová, S., Ježek, J., Šerý, M., Samek, O., Zemánek, P., Nedbal, L., & Trtílek, M. (2012) · Journal of Applied Phycology · doi:10.1007/s10811-011-9754-4 · Cited by 46 · see citing works
Supercontinuum Dynamically Visualizes a Dividing Single Cell
Kano, H., & Hamaguchi, H. (2007) · Analytical Chemistry · doi:10.1021/ac071416z · Cited by 45 · see citing works
Self-citationYue, L., Maeda, Y., Sunaga, Y., Muto, M., Matsumoto, M., Yoshino, T., & Tanaka, T. (2013) · Marine Drugs · doi:10.3390/md11125008 · Cited by 32 · see citing works
Self-citationMuto, M., Kubota, C., Tanaka, M., Satoh, A., Matsumoto, M., Yoshino, T., & Tanaka, T. (2013) · PLoS ONE · doi:10.1371/journal.pone.0073507 · Cited by 23 · see citing works
Self-citationMonitoring of cellular behaviors by microcavity array-based single-cell patterning
Osada, K., Hosokawa, M., Yoshino, T., & Tanaka, T. (2013) · The Analyst · doi:10.1039/c3an01698f · Cited by 18 · see citing works
Self-citation
Flagged first, then by citations
Cited by
Sorted by citations
He, Y., Wang, X., Ma, B., & Xu, J. (2019) · Biotechnology Advances · doi:10.1016/j.biotechadv.2019.04.010 · Cited by 98 · see citing works
Raman Spectroscopy and Its Modifications Applied to Biological and Medical Research
Allakhverdiev, E. S., Khabatova, V. V., Kossalbayev, B. D., Zadneprovskaya, E. V., Rodnenkov, O. V., Martynyuk, T. V., Maksimov, G. V., Alwasel, S., Tomo, T., & Allakhverdiev, S. I. (2022) · Cells · doi:10.3390/cells11030386 · Cited by 88 · see citing works
The Role of Raman Spectroscopy Within Quantitative Metabolomics
Lima, C. A., Muhamadali, H., & Goodacre, R. (2021) · Annual Review of Analytical Chemistry · doi:10.1146/annurev-anchem-091420-092323 · Cited by 84 · see citing works
An integrative Raman microscopy-based workflow for rapid in situ analysis of microalgal lipid bodies
Sharma, S. K., Nelson, D. R., Abdrabu, R., Khraiwesh, B., Jijakli, K., Arnoux, M., O’Connor, M. J., Bahmani, T., Cai, H., Khapli, S., et al. (2015) · Biotechnology for Biofuels · doi:10.1186/s13068-015-0349-1 · Cited by 80 · see citing works
He, Y., Zhang, P., Huang, S. W., Wang, T., Ji, Y., & Xu, J. (2017) · Biotechnology for Biofuels · doi:10.1186/s13068-017-0967-x · Cited by 78 · see citing works
Patel, A., Antonopoulou, I., Enman, J., Rova, U., Christakopoulos, P., & Μάτσακας, Λ. (2019) · BMC Chemical Engineering · doi:10.1186/s42480-019-0013-9 · Cited by 66 · see citing works
Mapping and Profiling Lipid Distribution in a 3D Model of Breast Cancer Progression
Vidavsky, N., Kunitake, J. A. M. R., Diaz-Rubio, M. E., Chiou, A. E., Loh, H.-C., Zhang, S., Mašić, A., Fischbach, C., & Estroff, L. A. (2019) · ACS Central Science · doi:10.1021/acscentsci.8b00932 · Cited by 65 · see citing works
Raman Scattering: From Structural Biology to Medical Applications
Vlasov, A. V., Maliar, N. L., Bazhenov, S. V., Nikelshparg, E. I., Brazhe, N. A., Vlasova, A. D., Osipov, S. D., Sudarev, V. V., Ryzhykau, Y. L., Bogorodskiy, A. O., et al. (2020) · Crystals · doi:10.3390/cryst10010038 · Cited by 58 · see citing works
Quantification of Polyphosphate in Microalgae by Raman Microscopy and by a Reference Enzymatic Assay
Moudříková, Š., Sadowsky, A., Metzger, S. U., Nedbal, L., Mettler‐Altmann, T., & Mojzeš, P. (2017) · Analytical Chemistry · doi:10.1021/acs.analchem.7b02393 · Cited by 53 · see citing works
Li, X., Sha, J., Chu, B., Wei, Y., Huang, W., Zhou, H., Xu, N., & He, Y. (2019) · Sensors and Actuators B: Chemical · doi:10.1016/j.snb.2019.04.048 · Cited by 52 · see citing works
Plant and algal lipidomes: Analysis, composition, and their societal significance
Jouhet, J., Alves, E., Boutté, Y., Darnet, S. H., Domergue, F., Durand, T., Fischer, P. J., Fouillen, L., Grūbe, M., Joubès, J., et al. (2024) · Progress in Lipid Research · doi:10.1016/j.plipres.2024.101290 · Cited by 52 · see citing works
Chiu, L., Ho, S., Shimada, R., Ren, N., & Ozawa, T. (2017) · Biotechnology for Biofuels · doi:10.1186/s13068-016-0691-y · Cited by 51 · see citing works
Jaeger, D., Pilger, C., Hachmeister, H., Oberländer, E., Wördenweber, R., Wichmann, J., Mussgnug, J. H., Huser, T., & Kruse, O. (2016) · Scientific Reports · doi:10.1038/srep35340 · Cited by 47 · see citing works
Li, K., Cheng, J., Ye, Q., He, Y., Zhou, J., & Cen, K. (2017) · Bioresource Technology · doi:10.1016/j.biortech.2017.04.116 · Cited by 50 · see citing works
Lipid Droplet Phase Transition in Freezing Cat Embryos and Oocytes Probed by Raman Spectroscopy
Okotrub, K. A., Mokrousova, V. I., Amstislavsky, S. Y., & Surovtsev, N. V. (2018) · Biophysical Journal · doi:10.1016/j.bpj.2018.06.019 · Cited by 39 · see citing works
A polyyne toxin produced by an antagonistic bacterium blinds and lyses a Chlamydomonad alga
Hotter, V. S., Zopf, D., Kim, H. J., Silge, A., Schmitt, M. P., Aiyar, P., Fleck, J., Matthäus, C., Hniopek, J., Yan, Q., et al. (2021) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2107695118 · Cited by 39 · see citing works
Raman Imaging from Microscopy to Nanoscopy, and to Macroscopy
Zheng, X., Zong, C., Xu, M., Wang, X., & Ren, B. W. (2015) · Small · doi:10.1002/smll.201403804 · Cited by 39 · see citing works
Bruggen, C. V., Punihaole, D., Keith, A. R., Schmitz, A. J., Tolar, J., Frontiera, R. R., & Reineke, T. M. (2020) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2016860117 · Cited by 38 · see citing works
Detection and imaging of lipids of Scenedesmus obliquus based on confocal Raman microspectroscopy
Shao, Y., Fang, H., Zhou, H., Wang, Q., Zhu, Y., & He, Y. (2017) · Biotechnology for Biofuels · doi:10.1186/s13068-017-0977-8 · Cited by 30 · see citing works
Lipids monitoring in Scenedesmus obliquus based on terahertz technology
Shao, Y., Gu, W., Qiu, Y., Wang, S., Peng, Y., Zhu, Y., & Zhuang, S. (2020) · Biotechnology for Biofuels · doi:10.1186/s13068-020-01801-0 · Cited by 30 · see citing works
Law, S., Asanuma, M., Shou, J., Ozeki, Y., Kodama, Y., & Numata, K. (2023) · JACS Au · doi:10.1021/jacsau.3c00041 · Cited by 21 · see citing works
New techniques in antibiotic discovery and resistance: Raman spectroscopy
CAREY, P. R., & Heidari‐Torkabadi, H. (2015) · Annals of the New York Academy of Sciences · doi:10.1111/nyas.12847 · Cited by 18 · see citing works
Moudříková, Š., Ivanov, I. N., Vítová, M., Nedbal, L., Zachleder, V., Mojzeš, P., & Bišová, K. (2021) · Cells · doi:10.3390/cells10010062 · Cited by 18 · see citing works
Okotrub, K. A., Amstislavsky, S. Y., & Surovtsev, N. V. (2017) · Archives of Biochemistry and Biophysics · doi:10.1016/j.abb.2017.10.001 · Cited by 18 · see citing works
Xu, F. X., Rathbone, E. G., & Fu, D. (2023) · The Journal of Physical Chemistry B · doi:10.1021/acs.jpcb.2c09105 · Cited by 16 · see citing works
Role of Lipid Droplets in the Development of Oocytes and Preimplantation Embryos in Mammals
Brusentsev, E. Y., Mokrousova, V. I., Igonina, T. N., Рожкова, И. Н., & Amstislavsky, S. Y. (2019) · Russian Journal of Developmental Biology · doi:10.1134/s1062360419050102 · Cited by 16 · see citing works
Karlo, J., Gupta, A., & Singh, S. P. (2024) · The Analyst · doi:10.1039/d4an00203b · Cited by 15 · see citing works
Mrđenović, D., Combes, B. F., Ni, R., Zenobi, R., & Kumar, N. (2023) · ACS Chemical Neuroscience · doi:10.1021/acschemneuro.3c00607 · Cited by 14 · see citing works
Verwee, E., Chaerle, P., Verduijn, J., Mienis, E., Sekulić, M., Keersmaecker, H. D., Vyverman, W., Foubert, I., Skirtach, A. G., & Damme, E. J. M. V. (2024) · Algal Research · doi:10.1016/j.algal.2024.103553 · Cited by 14 · see citing works
Rocha, W. F. D. C., Sheen, D. A., & Bearden, D. W. (2018) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-018-1240-2 · Cited by 14 · see citing works
Igonina, T. N., Okotrub, K. A., Brusentsev, E. Y., Chuyko, E. А., Ragaeva, D. S., Ranneva, S. V., & Amstislavsky, S. Y. (2021) · Cryobiology · doi:10.1016/j.cryobiol.2021.01.014 · Cited by 13 · see citing works
He, Y., Liu, X., Fang, H., Zhang, J., & Feng, X. (2021) · Journal of Chemistry · doi:10.1155/2021/8857984 · Cited by 12 · see citing works
Chen, L., Ma, N., Park, Y., Jin, S., Hwang, H., Jiang, D.-Y., & Jung, Y. M. (2017) · Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy · doi:10.1016/j.saa.2017.12.043 · Cited by 12 · see citing works
Microwell-assisted filtration with anodic aluminum oxide membrane for Raman analysis of algal cells
Wen, F.-Y., Chen, P.-S., Liao, T.-W., & Juang, Y. (2018) · Algal Research · doi:10.1016/j.algal.2018.06.022 · Cited by 11 · see citing works
LaLone, V., Mourão, M., Standiford, T. J., Raghavendran, K., Shedden, K., Stringer, K. A., & Rosania, G. R. (2018) · Pharmaceutical Research · doi:10.1007/s11095-018-2540-0 · Cited by 11 · see citing works
Schweikhard, V., Baral, A., Krishnamachari, V. V., Hay, W. C., & Fuhrmann, M. (2019) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/789248 · Cited by 11 · see citing works
Deniset‐Besseau, A., Coat, R., Moutel, B., Rebois, R., Mathurin, J., Grizeau, D., Dazzi, A., & Gonçalves, O. (2021) · Applied Spectroscopy · doi:10.1177/00037028211050659 · Cited by 8 · see citing works
Fluorescent Chemical Tools for Tracking Anionic Phospholipids
Kundu, R., Chandra, A., & Datta, A. (2021) · Israel Journal of Chemistry · doi:10.1002/ijch.202100003 · Cited by 8 · see citing works
Single-Cell Characterization of Microalgal Lipid Contents with Confocal Raman Microscopy
Abdrabu, R., Sharma, S. K., Khraiwesh, B., Jijakli, K., Nelson, D. R., Alzahmi, A. S., Koussa, J., Sultana, M., Khapli, S., Jagannathan, R., et al. (2016) · Series in bioengineering · doi:10.1007/978-3-662-49118-8_14 · Cited by 7 · see citing works
Chen, H., Chu, Z., Bai, Z., Hu, J., & Ma, J. (2025) · Industrial Crops and Products · doi:10.1016/j.indcrop.2025.121353 · Cited by 5 · see citing works
Development of hydrogel-based standards and phantoms for non-linear imaging at depth
Haseeb, F., Bourdakos, K. N., Forsyth, E., Setchfield, K., Gorman, A., Venkateswaran, S., Wright, A. J., Mahajan, S., & Bradley, M. (2023) · Journal of Biomedical Optics · doi:10.1117/1.jbo.28.12.126007 · Cited by 6 · see citing works
Two-Dimensional and Three-Dimensional Raman Spectroscopic Imaging of Spirulina platensis Cells
Zhang, S., Gao, S., Chen, F., Pang, H., Gao, Y., & Wang, G. (2022) · Journal of Applied Spectroscopy · doi:10.1007/s10812-022-01453-y · Cited by 2 · see citing works
Lipid droplets-vacuoles interaction promotes lipophagy in the oleaginous diatom Fistulifera solaris
Tanaka, T., Moriya, S., Nonoyama, T., Maeda, Y., Kaha, M., Yoshino, T., Matsumoto, M., & Bowler, C. (2024) · Algal Research · doi:10.1016/j.algal.2024.103481 · Cited by 2 · see citing works
Self-citationVibrational Spectroscopy in Bioanalysis
Mamián-López, M. B., & Paschoal, V. H. (2021) · Springer eBooks · doi:10.1007/978-3-030-82381-8_6 · Cited by 2 · see citing works
Subtracting the Raman Spectrum of Solvent for Quantitative Analysis
Zhang, F., Sun, K., Jiang, Y., Jia, G., Fan, F., & Li, C. (2025) · Journal of Raman Spectroscopy · doi:10.1002/jrs.6844 · Cited by 1 · see citing works
Zhan, B., Tan, Z., Li, X., Zhang, S., Wu, Y., Zhang, H., & Luo, W. (2025) · Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy · doi:10.1016/j.saa.2025.127009 · Cited by 1 · see citing works
Decoding the Biochemical Complexities Using Raman Spectroscopy
Karlo, J., Giriraju, D., Mahanta, N., & Singh, S. P. (2026) · Biochemistry · doi:10.1021/acs.biochem.6c00389 · Cited by 1 · see citing works
- Authors
- Hosokawa, M., Ando, M., Mukai, S., Osada, K., Yoshino, T., Hamaguchi, H.-O., & Tanaka, T.
- Affiliations
- Waseda University (JP)
- Tokyo University of Agriculture and Technology (JP)
- National Yang Ming Chiao Tung University (TW)
- Japan Science and Technology Agency (JP)
- Corresponding author
- Hamaguchi, Hiro-o
- Tanaka, Tsuyoshi
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2014-08-08
- Volume
- 86
- Issue
- 16
- Pages
- 8224-8230
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2062076010
- Cited by
- 47
- References
- 43
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 4 · 2023: 4 · 2022: 2 · 2021: 8 · 2020: 3 · 2019: 6 · 2018: 4 · 2017: 7 · 2016: 2 · 2015: 3
- FWCI
- 2.37
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Analytical Chemistry (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Japan Society for the Promotion of Science (23360363)
- Core Research for Evolutional Science and Technology, Japan Science and Technology Agency
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Spectroscopy Techniques in Biomedical and Chemical Research
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Keywords
- lipid quantification, triglycerides, oleaginous microalgae, fatty acid composition, fatty acid chain length, degree of unsaturation, label-free imaging, microalgal lipid accumulation, biodiesel production, lipid metabolism
- MeSH terms
- Equipment Design, Fatty Acids, Lipids, Spectrum Analysis, Raman, Triglycerides, Microalgae, Single-Cell Analysis
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
