Cited by 83Spectroscopy Techniques in Biomedical and Chemical ResearchAlgal biology and biofuel productionSpectroscopy and Quantum Chemical Studies

Raman Spectroscopy Analysis of Botryococcene Hydrocarbons from the Green Microalga Botryococcus braunii

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Weiss, Taylor L., Chun, Hye Jin, Okada, Shigeru, Vitha, Stanislav, Holzenburg, Andreas, Laane, Jaan, Devarenne, Timothy P.·2010·Journal of Biological Chemistry

Abstract

Botryococcus braunii, B race is a unique green microalga that produces large amounts of liquid hydrocarbons known as botryococcenes that can be used as a fuel for internal combustion engines. The simplest botryococcene (C(30)) is metabolized by methylation to give intermediates of C(31), C(32), C(33), and C(34), with C(34) being the predominant botryococcene in some strains. In the present work we have used Raman spectroscopy to characterize the structure of botryococcenes in an attempt to identify and localize botryococcenes within B. braunii cells. The spectral region from 1600-1700 cm(-1) showed ν(C=C) stretching bands specific for botryococcenes. Distinct botryococcene Raman bands at 1640 and 1647 cm(-1) were assigned to the stretching of the C=C bond in the botryococcene branch and the exomethylene C=C bonds produced by the methylations, respectively. A Raman band at 1670 cm(-1) was assigned to the backbone C=C bond stretching. Density function theory calculations were used to determine the Raman spectra of all botryococcenes to compare computed theoretical values with those observed. The analysis showed that the ν(C=C) stretching bands at 1647 and 1670 cm(-1) are actually composed of several closely spaced bands arising from the six individual C=C bonds in the molecule. We also used confocal Raman microspectroscopy to map the presence and location of methylated botryococcenes within a colony of B. braunii cells based on the methylation-specific 1647 cm(-1) botryococcene Raman shift.

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Crossref · 10.1074/jbc.m110.157230

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Authors
Weiss, T. L., Chun, H. J., Okada, S., Vitha, S., Holzenburg, A., Laane, J., & Devarenne, T. P.
Affiliations
  • Texas A&M University (US)
  • The University of Tokyo (JP)
Corresponding author
Devarenne, Timothy P.
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
2010-10
Volume
285
Issue
42
Pages
32458-32466
ISSN
0021-9258 (ISSN-L)
OpenAlex
W2004393345
Cited by
83
References
57
Citations by year
2025: 5 · 2024: 3 · 2023: 3 · 2022: 1 · 2021: 5 · 2020: 1 · 2019: 10 · 2018: 3 · 2017: 6 · 2016: 9 · 2015: 5 · 2014: 10 · 2013: 7 · 2012: 7
FWCI
4.26
Type
journal article
Language
en
Open access
Locations
  • Journal of Biological Chemistry (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Spectroscopy Techniques in Biomedical and Chemical Research
  • Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
  • Physical Sciences › Physics and Astronomy › Atomic and Molecular Physics, and Optics › Spectroscopy and Quantum Chemical Studies
Keywords
Botryococcus braunii, botryococcenes, green microalgae, liquid hydrocarbons, Raman band assignment, DFT calculations, confocal Raman spectroscopy, biofuels
MeSH terms
Inclusion Bodies, Hydrocarbons, Spectrum Analysis, Raman, Squalene, Molecular Structure, Microalgae

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