The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
Observation of the Generation of ONOO– in Mitochondria under Various Stimuli with a Sensitive Fluorescence Probe
Abstract
Peroxynitrite (ONOO – ), an important reactive oxygen species (ROS), may be generated in the mitochondria under various physiological and pathological conditions. In this work, we designed and synthesized a mitochondria-targetable fluorescence probe RPTPP by using rhodamine as the fluorophore, phenylhydrazine as the recognition moiety, and triphenyl-phosphonium cation as the mitochondria-targeting moiety. Upon reaction of the probe with ONOO –, the oxidation of phenylhydrazine by ONOO – and the subsequent hydrolysis opens the nonfluorescent spirocyclic structure and, thus, triggers a fluorescence turn-on response, which provides a sensitive and selective method for the detection of ONOO – . The mitochondria-targeting property of RPTPP was confirmed by the colocalization experiments as well as the mitochondria uncoupling treatments. Moreover, the applications of the probe for imaging intracellular ONOO – were performed in living cells, which reveals that the ONOO – level in RAW264.7 cells undergoes an about 9-fold increase with the stimulation of LPS and IFN-γ for 15 h.
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Beckman, J. S., Beckman, T. W., Chen, J., Marshall, P. A., & Freeman, B. A. (1990) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.87.4.1620 · Cited by 7,029
Chemical biology of peroxynitrite: Kinetics, diffusion, and radicals
Ferrer-Sueta, G., & Radi, R. (2009) · ACS Chemical Biology · doi:10.1021/cb800279q · Cited by 776
HeLa cells 50 years on: the good, the bad and the ugly
Masters, J. R. (2002) · Nature Reviews Cancer · doi:10.1038/nrc775 · Cited by 560
Mitochondria-Targeted Small Molecule Therapeutics and Probes
Smith, R. A. J., Hartley, R. C., & Murphy, M. P. (2011) · Antioxidants & Redox Signaling · doi:10.1089/ars.2011.3969 · Cited by 399
The formation of peroxynitrite in vivo from nitric oxide and superoxide
Squadrito, G. L., & Pryor, W. A. (1995) · Chemico-Biological Interactions · doi:10.1016/0009-2797(94)03591-u · Cited by 200
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Gao, P., Pan, W., Li, N., & Tang, B. (2019) · Chemical Science · doi:10.1039/c9sc01652j · Cited by 599
Jiao, X., Li, Y., Niu, J., Xie, X., Wang, X., & Tang, B. (2018) · Analytical Chemistry · doi:10.1021/acs.analchem.7b04234 · Cited by 484
Cheng, D., Peng, J., Lv, Y., Su, D., Liu, D., Chen, M., Yuan, L., & Zhang, X. (2019) · Journal of the American Chemical Society · doi:10.1021/jacs.9b01374 · Cited by 308
Ye, M., Xiang, Y., Gong, J., Wang, X., Mao, Z., & Liu, Z. (2023) · Journal of Hazardous Materials · doi:10.1016/j.jhazmat.2022.130612 · Cited by 37
Near-infrared fluorescent Aza-BODIPY dyes: Rational structural design and biomedical imaging
Zhao, B., Liao, L., Zhu, Y., Hu, Z., & Wu, F. (2023) · Journal of Luminescence · doi:10.1016/j.jlumin.2023.120099 · Cited by 31
Metadata
- Authors
- Li, H., Li, X., Wu, X., Shi, W., & Ma, H.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2017-05-02
- Volume
- 89
- Issue
- 10
- Pages
- 5519-5525
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2607851096
- Cited by
- 181
- References
- 5
- FWCI
- 10.11
- Type
- journal article
- Language
- en
- Open access
- closed
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