The DOI matches its Crossref record; 38 of the 38 references it cites were found in a registry or an index.
Enzymatic Purification of Microplastics in Environmental Samples
Open accessAbstract
Micro-Fourier transform infrared (micro-FTIR) spectroscopy and Raman spectroscopy enable the reliable identification and quantification of microplastics (MPs) in the lower micron range. Since concentrations of MPs in the environment are usually low, the large sample volumes required for these techniques lead to an excess of coenriched organic or inorganic materials. While inorganic materials can be separated from MPs using density separation, the organic fraction impedes the ability to conduct reliable analyses. Hence, the purification of MPs from organic materials is crucial prior to conducting an identification via spectroscopic techniques. Strong acidic or alkaline treatments bear the danger of degrading sensitive synthetic polymers. We suggest an alternative method, which uses a series of technical grade enzymes for purifying MPs in environmental samples. A basic enzymatic purification protocol (BEPP) proved to be efficient while reducing 98.3 ± 0.1% of the sample matrix in surface water samples. After showing a high recovery rate (84.5 ± 3.3%), the BEPP was successfully applied to environmental samples from the North Sea where numbers of MPs range from 0.05 to 4.42 items m –3 . Experiences with different environmental sample matrices were considered in an improved and universally applicable version of the BEPP, which is suitable for focal plane array detector (FPA)-based micro-FTIR analyses of water, wastewater, sediment, biota, and food samples.
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Cites
Lost at sea: where is all the plastic?
Thompson, R. C., Olsen, Y., Mitchell, R. P., Davis, A., Rowland, S. J., John, A. W. G., McGonigle, D., & Russell, A. E. (2004) · Science · doi:10.1126/science.1094559 · Cited by 7,765
Hidalgo-Ruz, V., Gutow, L., Thompson, R. C., & Thiel, M. (2012) · Environmental Science & Technology · doi:10.1021/es2031505 · Cited by 5,440
Plastic debris in the open ocean
Cózar, A., Echevarría, F., González-Gordillo, J. I., Irigoien, X., Úbeda, B., Hernández-León, S., Palma, Á. T., Navarro, S., García-de-Lomas, J., Ruiz, A., et al. (2014) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1314705111 · Cited by 3,133
Eerkes-Medrano, D., Thompson, R. C., & Aldridge, D. C. (2015) · Water Research · doi:10.1016/j.watres.2015.02.012 · Cited by 2,638
Microplastics in freshwater ecosystems: what we know and what we need to know
Wagner, M., Scherer, C., Alvarez-Muñoz, D., Brennholt, N., Bourrain, X., Buchinger, S., Fries, E., Grosbois, C., Klasmeier, J., Marti, T., et al. (2014) · Environmental Sciences Europe · doi:10.1186/s12302-014-0012-7 · Cited by 1,543
Mintenig, S. M., Int-Veen, I., Löder, M. G. J., Primpke, S., & Gerdts, G. (2017) · Water Research · doi:10.1016/j.watres.2016.11.015 · Cited by 1,481
Self-citationOccurrence and distribution of microplastics in marine sediments along the Belgian coast
Claessens, M., Meester, S. D., Landuyt, L. V., Clerck, K. D., & Janssen, C. R. (2011) · Marine Pollution Bulletin · doi:10.1016/j.marpolbul.2011.06.030 · Cited by 1,428
A new analytical approach for monitoring microplastics in marine sediments
Nuelle, M.-T., Dekiff, J. H., Remy, D., & Fries, E. (2014) · Environmental Pollution · doi:10.1016/j.envpol.2013.07.027 · Cited by 1,401
Microplastics in sediments: A review of techniques, occurrence and effects
Van Cauwenberghe, L., Devriese, L., Galgani, F., Robbens, J., & Janssen, C. R. (2015) · Marine Environmental Research · doi:10.1016/j.marenvres.2015.06.007 · Cited by 1,245
Analysis of environmental microplastics by vibrational microspectroscopy: FTIR, Raman or both?
Käppler, A., Fischer, D., Oberbeckmann, S., Schernewski, G., Labrenz, M., Eichhorn, K.-J., & Voit, B. (2016) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-016-9956-3 · Cited by 1,095
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Koelmans, A. A., Mohamed Nor, N. H., Hermsen, E., Kooi, M., Mintenig, S. M., & De France, J. (2019) · Water Research · doi:10.1016/j.watres.2019.02.054 · Cited by 2,535
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Ivleva, N. P. (2021) · Chemical Reviews · doi:10.1021/acs.chemrev.1c00178 · Cited by 1,172
Review of micro- and nanoplastic contamination in the food chain
Toussaint, B., Raffael, B., Angers-Loustau, A., Gilliland, D., Kestens, V., Petrillo, M., Rio-Echevarria, I. M., & Van den Eede, G. (2019) · Food Additives & Contaminants: Part A · doi:10.1080/19440049.2019.1583381 · Cited by 643
Primpke, S., Wirth, M., Lorenz, C., & Gerdts, G. (2018) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-018-1156-x · Cited by 584
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Tirkey, A., & Upadhyay, L. S. B. (2021) · Marine Pollution Bulletin · doi:10.1016/j.marpolbul.2021.112604 · Cited by 445
Raman microspectroscopy as a tool for microplastic particle analysis
Anger, P. M., von der Esch, E., Baumann, T., Elsner, M., Niessner, R., & Ivleva, N. P. (2018) · TrAC Trends in Analytical Chemistry · doi:10.1016/j.trac.2018.10.010 · Cited by 341
Microplastics in Food: A Review on Analytical Methods and Challenges
Kwon, J.-H., Kim, J.-W., Pham, T. D., Tarafdar, A., Hong, S., Chun, S.-H., Lee, S.-H., Kang, D.-Y., Kim, J.-Y., Kim, S.-B., et al. (2020) · International Journal of Environmental Research and Public Health · doi:10.3390/ijerph17186710 · Cited by 308
Morgana, S., Ghigliotti, L., Estévez-Calvar, N., Stifanese, R., Wieckzorek, A., Doyle, T., Christiansen, J. S., Faimali, M., & Garaventa, F. (2018) · Environmental Pollution · doi:10.1016/j.envpol.2018.08.001 · Cited by 289
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Fojt, J., David, J., Přikryl, R., Řezáčová, V., & Kučerík, J. (2020) · Science of The Total Environment · doi:10.1016/j.scitotenv.2020.140975 · Cited by 165
From the Well to the Bottle: Identifying Sources of Microplastics in Mineral Water
Weisser, J., Beer, I., Hufnagl, B., Hofmann, T., Lohninger, H., Ivleva, N. P., & Glas, K. (2021) · Water · doi:10.3390/w13060841 · Cited by 134
Jakubowicz, I., Enebro, J., & Yarahmadi, N. (2021) · Polymer Testing · doi:10.1016/j.polymertesting.2020.106953 · Cited by 108
Documentation of Microplastics in Tissues of Wild Coastal Animals
Haave, M., Gomiero, A., Schönheit, J., Nilsen, H., & Olsen, A. B. (2021) · Frontiers in Environmental Science · doi:10.3389/fenvs.2021.575058 · Cited by 80
Microplastic in Water and Sediments at the Confluence of the Elbe and Mulde Rivers in Germany
Laermanns, H., Reifferscheid, G., Kruse, J., Földi, C., Dierkes, G., Schaefer, D., Scherer, C., Bogner, C., & Stock, F. (2021) · Frontiers in Environmental Science · doi:10.3389/fenvs.2021.794895 · Cited by 63
Plastic pollution risks in bioretention systems: a case study
Mbachu, O., Kaparaju, P., & Pratt, C. (2023) · Environmental Technology · doi:10.1080/09593330.2022.2034984 · Cited by 23
Heinze, W. M., Lahive, E., Leicht, K., Mitrano, D. M., & Cornelis, G. (2026) · Geoderma · doi:10.1016/j.geoderma.2026.117768 · Cited by 3
Metadata
- Authors
- Löder, M. G. J., Imhof, H. K., Ladehoff, M., Löschel, L. A., Lorenz, C., Mintenig, S., Piehl, S., Primpke, S., Schrank, I., Laforsch, C., & Gerdts, G.
- Venue
- Environmental Science & Technology
- Publisher
- American Chemical Society (ACS)
- Published
- 2017-12-04
- Volume
- 51
- Issue
- 24
- Pages
- 14283-14292
- ISSN
- 0013-936X (ISSN-L)
- 1520-5851
- OpenAlex
- W2767480030
- Cited by
- 544
- References
- 38
- FWCI
- 13.04
- Type
- journal article
- Language
- en
- Open access
- green
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