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Antioxidant Activity/Capacity Measurement. 1. Classification, Physicochemical Principles, Mechanisms, and Electron Transfer (ET)-Based Assays

Open access
Apak, Reşat, Özyürek, Mustafa, Güçlü, Kubilay, Çapanoğlu, Esra·2016·Journal of Agricultural and Food Chemistry

Abstract

Because there is no widely adopted "total antioxidant parameter" as a nutritional index for labeling food and biological fluids, it is desirable to establish and standardize methods that can measure the total antioxidant capacity (TAC) level directly from plant-based food extracts and biological fluids. In this review, we (i) present and classify the widely used analytical approaches (e.g., in vitro and in vivo, enzymatic and nonenzymatic, electron transfer (ET)- and hydrogen atom transfer (HAT)-based, direct and indirect assays) for evaluating antioxidant capacity/activity; (ii) discuss total antioxidant capacity/activity assays in terms of chemical kinetics and thermodynamics, reaction mechanisms, and analytical performance characteristics, together with advantages and drawbacks; and (iii) critically evaluate ET-based methods for analytical, food chemical, biomedical/clinical, and environmental scientific communities so that they can effectively use these assays in the correct places to meet their needs.

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Metadata

Authors
Apak, R., Özyürek, M., Güçlü, K., & Çapanoğlu, E.
Venue
Journal of Agricultural and Food Chemistry
Published
2016-01-27
Volume
64
Issue
5
Pages
997-1027
ISSN
  • 0021-8561 (ISSN-L)
  • 1520-5118
OpenAlex
W2228243764
Cited by
972
References
10
FWCI
35.75
Type
journal article
Language
en
Open access
hybrid

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CoChat. (2026). Citation check: Antioxidant Activity/Capacity Measurement. 1. Classification, Physicochemical Principles, Mechanisms, and Electron Transfer (ET)-Based Assays. https://app.cochat.ai/papers/10.1021/acs.jafc.5b04739
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Apak, R., Özyürek, M., Güçlü, K., & Çapanoğlu, E. (2016). Antioxidant Activity/Capacity Measurement. 1. Classification, Physicochemical Principles, Mechanisms, and Electron Transfer (ET)-Based Assays. Journal of Agricultural and Food Chemistry. https://doi.org/10.1021/acs.jafc.5b04739

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Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.