Isolation and characterization of blueberry polyphenolic components and their effects on gut barrier dysfunction
Abstract
Highbush blueberries contain anthocyanins and proanthocyanidins that have antimicrobial and anti-inflammatory bioactivities. We isolated and characterized three polyphenolic fractions, a total polyphenol fraction (TPF), an anthocyanin-enriched fraction (AEF), and a proanthocyanidin-enriched fraction (PEF), from freeze-dried blueberry powder and evaluated their effects on an in vitro model of gut barrier dysfunction. High-performance liquid chromatography chromatograms illustrate successful fractionation of the blueberry powder into TPF, AEF, and PEF. AEF contained 21 anthocyanins, and PEF contained proanthocyanidin oligomers of (epi)catechin with primarily B-type interflavan bonds. The model uses a strain of Escherichia coli to disrupt a Caco-2 cell monolayer on Transwell inserts. Barrier function was measured by transepithelial electrical resistance (TEER), a marker of membrane permeability. All fractions were able to restore TEER values after an E. coli challenge when compared to the control, while AEF was able to attenuate the E. coli -induced decrease in TEER in a dose-dependent manner.
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Anthocyanins and proanthocyanidins from blueberries ameliorated E. coli-induced gut barrier dysfunction.
“Moreover, anthocyanins and proanthocyanidins, polyphenols form blueberries, ameliorated E. coli–induced gut barrier dysfunction.152”
From Regulation of Intestinal Barrier Function by Microbial Metabolites (2021) · cites this paper as [152]
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Regulation of Intestinal Barrier Function by Microbial Metabolites
Ghosh, S., Whitley, C. S., Haribabu, B., & Jala, V. R. (2021) · Cellular and Molecular Gastroenterology and Hepatology · doi:10.1016/j.jcmgh.2021.02.007 · Cited by 835 · see citing works
- Authors
- Polewski, M. A., Esquivel-Alvarado, D., Wedde, N. S., Kruger, C. G., & Reed, J. D.
- Affiliations
- University of Wisconsin–Madison (US)
- Reed Research Group, Department of Animal Sciences, University of Wisconsin—Madison, 1675 Observatory Drive, Madison, Wisconsin 53706, United States
- Corresponding author
- Polewski, Michael A.
- Venue
- Journal of Agricultural and Food Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2019-06-14
- Volume
- 68
- Issue
- 10
- Pages
- 2940-2947
- ISSN
- 0021-8561 (ISSN-L)
- 1520-5118
- OpenAlex
- W2950007012
- Cited by
- 36
- References
- 39
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 7 · 2023: 5 · 2022: 8 · 2021: 6 · 2020: 6
- FWCI
- 3.00
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Journal of Agricultural and Food Chemistry (publishedVersion)
- PubMed (publishedVersion)
- Funders
- U.S. Highbush Blueberry Council (MSN194682)
- Topics
- Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
- Keywords
- blueberry polyphenols, anthocyanins, proanthocyanidins, intestinal barrier dysfunction, Caco-2 cell monolayers, Escherichia coli, transepithelial electrical resistance, antimicrobial activity, anti-inflammatory activity
- MeSH terms
- Escherichia coli, Fruit, Humans, Intestinal Mucosa, Plant Extracts, Caco-2 Cells, Blueberry Plants, Polyphenols
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