The DOI matches its Crossref record; 88 of the 88 references it cites were found in a registry or an index.
CRISPRi and CRISPRa Screens in Mammalian Cells for Precision Biology and Medicine
Open accessAbstract
Next-generation DNA sequencing technologies have led to a massive accumulation of genomic and transcriptomic data from patients and healthy individuals. The major challenge ahead is to understand the functional significance of the elements of the human genome and transcriptome, and implications for diagnosis and treatment. Genetic screens in mammalian cells are a powerful approach to systematically elucidating gene function in health and disease states. In particular, recently developed CRISPR/Cas9-based screening approaches have enormous potential to uncover mechanisms and therapeutic strategies for human diseases. The focus of this review is the use of CRISPR interference (CRISPRi) and CRISPR activation (CRISPRa) for genetic screens in mammalian cells. We introduce the underlying technology and present different types of CRISPRi/a screens, including those based on cell survival/proliferation, sensitivity to drugs or toxins, fluorescent reporters, and single-cell transcriptomes. Combinatorial screens, in which large numbers of gene pairs are targeted to construct genetic interaction maps, reveal pathway relationships and protein complexes. We compare and contrast CRISPRi and CRISPRa with alternative technologies, including RNA interference (RNAi) and CRISPR nuclease-based screens. Finally, we highlight challenges and opportunities ahead.
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Citations
Cites
1 retracted
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Cited by
CRISPR-Cas guides the future of genetic engineering
Knott, G. J., & Doudna, J. A. (2018) · Science · doi:10.1126/science.aat5011 · Cited by 1,410
CRISPR technology: A decade of genome editing is only the beginning
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CRISPR Interference-Based Platform for Multimodal Genetic Screens in Human iPSC-Derived Neurons
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Self-citationSynthetic lethality as an engine for cancer drug target discovery
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Self-citationCRISPR Approaches to Small Molecule Target Identification
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From Microbial Switches to Metabolic Sensors: Rewiring the Gut–Brain Kynurenine Circuit
Tanaka, M., & Vécsei, L. (2025) · doi:10.20944/preprints202506.0880.v1 · Cited by 24
Current advances in CRISPR-Cas-mediated gene editing and regulation in cyanobacteria
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Metadata
- Authors
- Kampmann, M.
- Venue
- ACS Chemical Biology
- Publisher
- American Chemical Society (ACS)
- Published
- 2017-10-24
- Volume
- 13
- Issue
- 2
- Pages
- 406-416
- ISSN
- 1554-8929 (ISSN-L)
- 1554-8937
- OpenAlex
- W2765809583
- Cited by
- 373
- References
- 88
- FWCI
- 7.57
- Type
- journal article
- Language
- en
- Open access
- green
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