The DOI matches its Crossref record; 29 of the 29 references it cites were found in a registry or an index.
Cellular Uptake Mechanism of an Inorganic Nanovehicle and Its Drug Conjugates: Enhanced Efficacy Due To Clathrin-Mediated Endocytosis
Abstract
We present the mechanism for the cellular uptake of layered double hydroxide (LDH) nanoparticles that are internalized into MNNG/HOS cells principally via clathrin-mediated endocytosis. The intracellular LDHs are highly colocalized with not only typical endocytic proteins, such as clathrin heavy chain, dynamin, and eps15, but also transferrin, a marker of the clathrin-mediated process, suggesting their specific internalization pathway. LDHs loaded with an anticancer drug (MTX-LDH) were also prepared to confirm the efficacy of LDHs as drug delivery systems. The cellular uptake of MTX was higher in MTX-LDH-treated cells than in MTX-treated cells, giving a lower IC50 value for MTX-LDH than for MTX only. The inhibition of the cell cycle was greater for MTX-LDH than for MTX only. This result clearly shows that the internalization of LDH nanoparticles via clathrin-mediated endocytosis may allow the efficient delivery of MTX-LDH in cells and thus enhance drug efficacy.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
1 retracted
A Magnetic Nanoprobe Technology for Detecting Molecular Interactions in Live Cells
Won, J., Kim, M., Yi, Y.-W., Kim, Y. H., Jung, N., & Kim, T. K. (2005) · Science · doi:10.1126/science.1112869 · Cited by 149
RetractedNanomedicine: current status and future prospects
Moghimi, S. M., Hunter, A. C., & Murray, J. C. (2005) · The FASEB Journal · doi:10.1096/fj.04-2747rev · Cited by 2,029
Lai, C.-Y., Trewyn, B. G., Jeftinija, D. M., Jeftinija, K., Xu, S., Jeftinija, S., & Lin, V. S.-Y. (2003) · Journal of the American Chemical Society · doi:10.1021/ja028650l · Cited by 1,678
Carbon Nanotubes as Intracellular Protein Transporters: Generality and Biological Functionality
Kam, N. W. S., & Dai, H. (2005) · Journal of the American Chemical Society · doi:10.1021/ja050062v · Cited by 1,047
Kam, N. W. S., Liu, Z., & Dai, H. (2006) · Angewandte Chemie International Edition · doi:10.1002/anie.200503389 · Cited by 893
Self-Assembled Nanoparticle Probes for Recognition and Detection of Biomolecules
Maxwell, D. J., Taylor, J. R., & Nie, S. (2002) · Journal of the American Chemical Society · doi:10.1021/ja025814p · Cited by 890
Preparation and catalytic properties of cationic and anionic clays
Vaccari, A. (1998) · Catalysis Today · doi:10.1016/s0920-5861(98)00038-8 · Cited by 858
Zhao, W., Gu, J., Zhang, L., Chen, H., & Shi, J. (2005) · Journal of the American Chemical Society · doi:10.1021/ja051113r · Cited by 762
Intercalative Nanohybrids of Nucleoside Monophosphates and DNA in Layered Metal Hydroxide
Choy, J.-H., Kwak, S.-Y., Park, J.-S., Jeong, Y.-J., & Portier, J. (1999) · Journal of the American Chemical Society · doi:10.1021/ja981823f · Cited by 653
Self-citationAndersson, J., Rosenholm, J., Areva, S., & Lindén, M. (2004) · Chemistry of Materials · doi:10.1021/cm0401490 · Cited by 605
Kirchhausen, T. (2000) · Nature Reviews Molecular Cell Biology · doi:10.1038/35043117 · Cited by 560
Anticancer drug targets: cell cycle and checkpoint control
Shapiro, G. I., & Harper, J. W. (1999) · Journal of Clinical Investigation · doi:10.1172/jci9054 · Cited by 464
Layered double hydroxide as an efficient drug reservoir for folate derivatives
Choy, J.-H., Jung, J.-S., Oh, J.-M., Park, M., Jeong, J., Kang, Y.-K., & Han, O.-J. (2004) · Biomaterials · doi:10.1016/j.biomaterials.2003.09.083 · Cited by 435
Self-citationInhibition of clathrin-coated pit assembly by an Eps15 mutant
Benmerah, A., Bayrou, M., Cerf-Bensussan, N., & Dautry-Varsat, A. (1999) · Journal of Cell Science · doi:10.1242/jcs.112.9.1303 · Cited by 423
Intracellular trafficking of nonviral vectors
Medina-Kauwe, L. K., Xie, J., & Hamm-Alvarez, S. (2005) · Gene Therapy · doi:10.1038/sj.gt.3302592 · Cited by 346
Anticancer Antifolates: Current Status and Future Directions
McGuire, J. (2003) · Current Pharmaceutical Design · doi:10.2174/1381612033453712 · Cited by 305
Choy, J. ‐., Jang, E. ‐., Won, J. ‐., Chung, J. ‐., Jang, D. ‐., & Kim, Y. ‐. (2003) · Advanced Materials · doi:10.1002/adma.200305327 · Cited by 299
Self-citationInorganic Layered Double Hydroxides as Nonviral Vectors
Choy, J.-H., Kwak, S.-Y., Jeong, Y.-J., & Park, J.-S. (2000) · Angewandte Chemie · doi:10.1002/1521-3757(20001117)112:22<4207::aid-ange4207>3.0.co;2-h · Cited by 288
Self-citationPortet, D., Denizot, B., Rump, E., Lejeune, J.-J., & Jallet, P. (2001) · Journal of Colloid and Interface Science · doi:10.1006/jcis.2001.7500 · Cited by 281
Tat Peptide Directs Enhanced Clearance and Hepatic Permeability of Magnetic Nanoparticles
Wunderbaldinger, P., Josephson, L., & Weissleder, R. (2002) · Bioconjugate Chemistry · doi:10.1021/bc015563u · Cited by 266
High-dose methotrexate: a critical reappraisal.
Ackland, S. P., & Schilsky, R. L. (1987) · Journal of Clinical Oncology · doi:10.1200/jco.1987.5.12.2017 · Cited by 254
Garrotes, Springs, Ratchets, and Whips: Putting Dynamin Models to the Test
Sever, S., Damke, H., & Schmid, S. L. (2000) · Traffic · doi:10.1034/j.1600-0854.2000.010503.x · Cited by 213
Possible exploitation of magnetic nanoparticle–cell interaction for biomedical applications
Berry, C. C. (2005) · J. Mater. Chem. · doi:10.1039/b409715g · Cited by 209
Transferrin As A Targeting Ligand for Liposomes and Anticancer Drugs
Singh, M. (1999) · Current Pharmaceutical Design · doi:10.2174/138161280506230110111312 · Cited by 181
Widera, A., Norouziyan, F., & Shen, W.-C. (2003) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2003.07.004 · Cited by 173
Efficient delivery of anticancer drug MTX through MTX-LDH nanohybrid system
Oh, J.-M., Park, M., Kim, S.-T., Jung, J.-Y., Kang, Y.-G., & Choy, J.-H. (2006) · Journal of Physics and Chemistry of Solids · doi:10.1016/j.jpcs.2006.01.033 · Cited by 165
Self-citationUptake of Silica-Coated Nanoparticles by HeLa Cells
Xing, X., He, X., Peng, J., Wang, K., & Tan, W. (2005) · Journal of Nanoscience and Nanotechnology · doi:10.1166/jnn.2005.199 · Cited by 128
Inorganic–Biomolecular Hybrid Nanomaterials as a Genetic Molecular Code System
Choy, J., Oh, J. ‐., Park, M., Sohn, K. ‐., & Kim, J. ‐. (2004) · Advanced Materials · doi:10.1002/adma.200400027 · Cited by 110
Self-citationInorganic Layered Double Hydroxides as Nonviral Vectors
Choy, J., Kwak, S., Jeong, Y., & Park, J. (2000) · Angewandte Chemie International Edition · Cited by 22
Self-citation
Research works printed but not linked: 11
- Choy J. H., 1998, High-T-c superconductors in the two-dimensional limit: [(Py-CnH2n + 1)(2)HgI4]-Bi2Sr2Cam-1CumOy (m = 1 and 2). Science 280, 1589−1592
- Usuki A., 2001, Nano Lett., 1, 10.1021/nl010015a · doi:10.1021/nl010015a
- Choy J. H., 1999, J. Am. Chem. Soc., 121
- Zhao W., 2005, J. Am. Chem. Soc., 127
- Lai C. Y., 2003, J. Am. Chem. Soc., 125
- Oh J. M., 2006, J. Phys. Chem. Solids., 67
- Kam N. W. S., 2005, J. Am. Chem. Soc., 127
- Choy J. H., 2001, J. Mater. Chem., 11
- Tarnawski A., 1999, The antacid Talcid adsorbs and neutralizes all proteins secreted by H. pylori including VacA cytotoxin: A new mechanism for its ulcer-healing action? Digestion 60, 449−455
- Oh J. M., 2002, The effect of synthetic conditions on tailoring the size of hydrotalcite particles. Solid State Ionics 151, 285−291
- Li H., 2002, Med. Res. Rev., 22
Cited by
Harush-Frenkel, O., Rozentur, E., Benita, S., & Altschuler, Y. (2008) · Biomacromolecules · doi:10.1021/bm700535p · Cited by 405
Hybrid materials based on clays for environmental and biomedical applications
Ruiz-Hitzky, E., Aranda, P., Darder, M., & Rytwo, G. (2010) · Journal of Materials Chemistry · doi:10.1039/c0jm00432d · Cited by 325
Co-delivery of siRNAs and anti-cancer drugs using layered double hydroxide nanoparticles
Li, L., Gu, W., Chen, J., Chen, W., & Xu, Z. P. (2014) · Biomaterials · doi:10.1016/j.biomaterials.2013.12.095 · Cited by 304
Cui, C., Xue, Y.-N., Wu, M., Zhang, Y., Yu, P., Liu, L., Zhuo, R.-X., & Huang, S.-W. (2013) · Biomaterials · doi:10.1016/j.biomaterials.2013.01.101 · Cited by 169
Enhancing the Clinical Potential of AAV Vectors by Capsid Engineering to Evade Pre-Existing Immunity
Bartel, M. (2011) · Frontiers in Microbiology · doi:10.3389/fmicb.2011.00204 · Cited by 108
Metadata
- Authors
- Oh, J.-M., Choi, S.-J., Kim, S.-T., & Choy, J.-H.
- Venue
- Bioconjugate Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2006-10-21
- Volume
- 17
- Issue
- 6
- Pages
- 1411-1417
- ISSN
- 1043-1802 (ISSN-L)
- 1520-4812
- OpenAlex
- W2016637754
- Cited by
- 254
- References
- 29
- FWCI
- 4.56
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
