The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
Laser Fabrication of Three-Dimensional CAD Scaffolds from Photosensitive Gelatin for Applications in Tissue Engineering
Abstract
In the present work, 3D CAD scaffolds for tissue engineering applications were developed starting from methacrylamide-modified gelatin (GelMOD) using two-photon polymerization (2PP). The scaffolds were cross-linked employing the biocompatible photoinitiator Irgacure 2959. Because gelatin is derived from collagen (i.e., the main constituent of the ECM), the developed materials mimic the cellular microenvironment from a chemical point of view. In addition, by applying the 2PP technique, structural properties of the cellular microenvironment can also be mimicked. Furthermore, in vitro degradation assays indicated that the enzymatic degradation capability of gelatin is preserved for the methacrylamide-modified derivative. An in depth morphological analysis of the 2PP-fabricated scaffolds demonstrated that the parameters of the CAD model are reproduced with great precision, including the ridge-like surface topography on the order of 1.5 μm. The developed scaffolds showed an excellent stability in culture medium. In a final part of the present work, the suitability of the developed scaffolds for tissue engineering applications was verified. The results indicated that the applied materials are suitable to support porcine mesenchymal stem cell adhesion and subsequent proliferation. Upon applying osteogenic stimulation, the seeded cells differentiated into the anticipated lineage. Energy dispersive X-ray (EDX) analysis showed the induced calcification of the scaffolds. The results clearly indicate that 2PP is capable of manufacturing precisely constructed 3D tissue engineering scaffolds using photosensitive polymers as starting material.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Cell-laden microengineered gelatin methacrylate hydrogels
Nichol, J. W., Koshy, S. T., Bae, H., Hwang, C. M., Yamanlar, S., & Khademhosseini, A. (2010) · Biomaterials · doi:10.1016/j.biomaterials.2010.03.064 · Cited by 2,377
Three-Dimensional Cell Culture Matrices: State of the Art
Lee, J., Cuddihy, M. J., & Kotov, N. A. (2008) · Tissue Engineering Part B: Reviews · doi:10.1089/teb.2007.0150 · Cited by 1,047
Ovsianikov, A., Malinauskas, M., Schlie, S., Chichkov, B., Gittard, S., Narayan, R., Löbler, M., Sternberg, K., Schmitz, K.-P., & Haverich, A. (2011) · Acta Biomaterialia · doi:10.1016/j.actbio.2010.10.023 · Cited by 234
Self-citationChoi, J.-W., Wicker, R., Lee, S.-H., Choi, K.-H., Ha, C.-S., & Chung, I. (2009) · Journal of Materials Processing Technology · doi:10.1016/j.jmatprotec.2009.05.004 · Cited by 200
Three-Dimensional Biodegradable Structures Fabricated by Two-Photon Polymerization
Claeyssens, F., Hasan, E. A., Gaidukeviciute, A., Achilleos, D. S., Ranella, A., Reinhardt, C., Ovsianikov, A., Shizhou, X., Fotakis, C., Vamvakaki, M., et al. (2009) · Langmuir · doi:10.1021/la803803m · Cited by 187
Self-citation
Cited by
Synthesis, properties, and biomedical applications of gelatin methacryloyl (GelMA) hydrogels
Yue, K., Trujillo-de Santiago, G., Alvarez, M. M., Tamayol, A., Annabi, N., & Khademhosseini, A. (2015) · Biomaterials · doi:10.1016/j.biomaterials.2015.08.045 · Cited by 3,146
“used 2PP laser fabrication to form complex GelMA microconstructs [44-46].”
BackgroundMethodAu, A. K., Huynh, W., Horowitz, L. F., & Folch, A. (2016) · Angewandte Chemie International Edition · doi:10.1002/anie.201504382 · Cited by 788
Ren, X., Feng, Y., Guo, J., Wang, H., Li, Q., Yang, J., Hao, X., Lv, J., Ma, N., & Li, W. (2015) · Chemical Society Reviews · doi:10.1039/c4cs00483c · Cited by 573
3D printing of functional microrobots
Li, J., & Pumera, M. (2021) · Chemical Society Reviews · doi:10.1039/d0cs01062f · Cited by 303
Ovsianikov, A., Mironov, V., Stampfl, J., & Liska, R. (2012) · Expert Review of Medical Devices · doi:10.1586/erd.12.48 · Cited by 160
Self-citation
Metadata
- Authors
- Ovsianikov, A., Deiwick, A., Van Vlierberghe, S., Dubruel, P., Möller, L., Dräger, G., & Chichkov, B.
- Venue
- Biomacromolecules
- Publisher
- American Chemical Society (ACS)
- Published
- 2011-03-02
- Volume
- 12
- Issue
- 4
- Pages
- 851-858
- ISSN
- 1525-7797 (ISSN-L)
- 1526-4602
- OpenAlex
- W2052137502
- Cited by
- 301
- References
- 5
- FWCI
- 14.22
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
