The DOI matches its Crossref record; 25 of the 25 references it cites were found in a registry or an index.
Environment-Responsive Block Copolymer Micelles with a Disulfide Cross-Linked Core for Enhanced siRNA Delivery
Abstract
A core-shell-type polyion complex (PIC) micelle with a disulfide cross-linked core was prepared through the assembly of iminothiolane-modified poly(ethylene glycol)-block-poly(L-lysine) [PEG-b-(PLL-IM)] and siRNA at a characteristic optimum mixing ratio. The PIC micelles showed a spherical shape of approximately 60 nm in diameter with a narrow distribution. The micellar structure was maintained at physiological ionic strength but was disrupted under reductive conditions because of the cleavage of disulfide cross-links, which is desirable for siRNA release in the intracellular reductive environment. Importantly, environment-responsive PIC micelles achieved 100-fold higher siRNA transfection efficacy compared with non-cross-linked PICs prepared from PEG-b-poly(L-lysine), which were not stable at physiological ionic strength. PICs formed with PEG-b-(PLL-IM) at nonoptimum ratios did not assemble into micellar structure and did not achieve gene silencing following siRNA transfection. These findings show the feasibility of core cross-linked PIC micelles as carriers for therapeutic siRNA and show that stable micellar structure is critical for effective siRNA delivery into target cells.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans
Fire, A., Xu, S., Montgomery, M. K., Kostas, S. A., Driver, S. E., & Mello, C. C. (1998) · Nature · doi:10.1038/35888 · Cited by 15,429
Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells
Elbashir, S. M., Harborth, J., Lendeckel, W., Yalcin, A., Weber, K., & Tuschl, T. (2001) · Nature · doi:10.1038/35078107 · Cited by 9,456
Boussif, O., Lezoualc'h, F., Zanta, M. A., Mergny, M. D., Scherman, D., Demeneix, B., & Behr, J. P. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.16.7297 · Cited by 6,247
Meister, A., & Anderson, M. E. (1983) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.52.070183.003431 · Cited by 5,680
Therapeutic silencing of an endogenous gene by systemic administration of modified siRNAs
Soutschek, J., Akinc, A., Bramlage, B., Charisse, K., Constien, R., Donoghue, M., Elbashir, S., Geick, A., Hadwiger, P., Harborth, J., et al. (2004) · Nature · doi:10.1038/nature03121 · Cited by 2,183
Interfering with disease: a progress report on siRNA-based therapeutics
de Fougerolles, A., Vornlocher, H.-P., Maraganore, J., & Lieberman, J. (2007) · Nature Reviews Drug Discovery · doi:10.1038/nrd2310 · Cited by 1,203
Potent and persistent in vivo anti-HBV activity of chemically modified siRNAs
Morrissey, D. V., Lockridge, J. A., Shaw, L., Blanchard, K., Jensen, K., Breen, W., Hartsough, K., Machemer, L., Radka, S., Jadhav, V., et al. (2005) · Nature Biotechnology · doi:10.1038/nbt1122 · Cited by 1,168
Ellman's reagent: 5,5′-dithiobis(2-nitrobenzoic acid)—a reexamination
Riddles, P. W., Blakeley, R. L., & Zerner, B. (1979) · Analytical Biochemistry · doi:10.1016/0003-2697(79)90792-9 · Cited by 1,015
Schiffelers, R. M. (2004) · Nucleic Acids Research · doi:10.1093/nar/gnh140 · Cited by 901
Harada, A., & Kataoka, K. (1995) · Macromolecules · doi:10.1021/ma00119a019 · Cited by 848
Self-citationProgress towards in Vivo Use of siRNAs
Behlke, M. A. (2006) · Molecular Therapy · doi:10.1016/j.ymthe.2006.01.001 · Cited by 677
Katayose, S., & Kataoka, K. (1997) · Bioconjugate Chemistry · doi:10.1021/bc9701306 · Cited by 469
Self-citationInhibition of influenza virus production in virus-infected mice by RNA interference
Ge, Q., Filip, L., Bai, A., Nguyen, T., Eisen, H. N., & Chen, J. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0402486101 · Cited by 444
Miyata, K., Kakizawa, Y., Nishiyama, N., Harada, A., Yamasaki, Y., Koyama, H., & Kataoka, K. (2004) · Journal of the American Chemical Society · doi:10.1021/ja0379666 · Cited by 394
Self-citationKakizawa, Y., Harada, A., & Kataoka, K. (1999) · Journal of the American Chemical Society · doi:10.1021/ja993057y · Cited by 331
Self-citationKataoka, K., Togawa, H., Harada, A., Yasugi, K., Matsumoto, T., & Katayose, S. (1996) · Macromolecules · doi:10.1021/ma961217+ · Cited by 303
Self-citationKakizawa, Y., Harada, A., & Kataoka, K. (2001) · Biomacromolecules · doi:10.1021/bm000142l · Cited by 256
Self-citationPolyion complex micelles as vectors in gene therapy – pharmacokinetics and in vivo gene transfer
Harada-Shiba, M., Yamauchi, K., Harada, A., Takamisawa, I., Shimokado, K., & Kataoka, K. (2002) · Gene Therapy · doi:10.1038/sj.gt.3301665 · Cited by 242
Self-citationThermodynamics of Oligoarginines Binding to RNA and DNA
Mascotti, D. P., & Lohman, T. M. (1997) · Biochemistry · doi:10.1021/bi970272n · Cited by 128
Miyata, K., Kakizawa, Y., Nishiyama, N., Yamasaki, Y., Watanabe, T., Kohara, M., & Kataoka, K. (2005) · Journal of Controlled Release · doi:10.1016/j.jconrel.2005.09.043 · Cited by 126
Self-citationItaka, K., Kanayama, N., Nishiyama, N., Jang, W., Yamasaki, Y., Nakamura, K., Kawaguchi, H., & Kataoka, K. (2004) · Journal of the American Chemical Society · doi:10.1021/ja047174r · Cited by 125
Self-citationHarada, A., Cammas, S., & Kataoka, K. (1996) · Macromolecules · doi:10.1021/ma960487p · Cited by 122
Self-citationDEWOLF, H., Snel, C. J., Verbaan, F. J., Schiffelers, R. M., Hennink, W. E., & Storm, G. (2006) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2006.10.029 · Cited by 112
Akagi, D., Oba, M., Koyama, H., Nishiyama, N., Fukushima, S., Miyata, T., Nagawa, H., & Kataoka, K. (2007) · Gene Therapy · doi:10.1038/sj.gt.3302945 · Cited by 100
Self-citationMonomolecular Assembly of siRNA and Poly(ethylene glycol)−Peptide Copolymers
DeRouchey, J. E., Schmidt, C., Walker, G. F., Koch, C. A., Plank, C., Wagner, E., & Rädler, J. O. (2008) · Biomacromolecules · doi:10.1021/bm7011482 · Cited by 68
Cited by
Polymers for Drug Delivery Systems
Liechty, W. B., Kryscio, D. R., Slaughter, B. V., & Peppas, N. A. (2010) · Annual Review of Chemical and Biomolecular Engineering · doi:10.1146/annurev-chembioeng-073009-100847 · Cited by 1,608
Block copolymer micelles in nanomedicine applications
Cabral, H., Miyata, K., Osada, K., & Kataoka, K. (2018) · Chemical Reviews · doi:10.1021/acs.chemrev.8b00199 · Cited by 1,309
Self-citationFleige, E., Quadir, M. A., & Haag, R. (2012) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2012.01.020 · Cited by 1,111
Strategies, design, and chemistry in siRNA delivery systems
Dong, Y., Siegwart, D. J., & Anderson, D. G. (2019) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2019.05.004 · Cited by 572
Talelli, M., Barz, M., Rijcken, C. J. F., Kiessling, F., Hennink, W. E., & Lammers, T. (2015) · Nano Today · doi:10.1016/j.nantod.2015.01.005 · Cited by 506
Metadata
- Authors
- Matsumoto, S., Christie, R. J., Nishiyama, N., Miyata, K., Ishii, A., Oba, M., Koyama, H., Yamasaki, Y., & Kataoka, K.
- Venue
- Biomacromolecules
- Publisher
- American Chemical Society (ACS)
- Published
- 2008-12-05
- Volume
- 10
- Issue
- 1
- Pages
- 119-127
- ISSN
- 1525-7797 (ISSN-L)
- 1526-4602
- OpenAlex
- W2015917934
- Cited by
- 327
- References
- 25
- FWCI
- 8.31
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
