The DOI matches its Crossref record; 25 of the 25 references it cites were found in a registry or an index.
Environment-Responsive Block Copolymer Micelles with a Disulfide Cross-Linked Core for Enhanced siRNA Delivery
Abstract
A core-shell-type polyion complex (PIC) micelle with a disulfide cross-linked core was prepared through the assembly of iminothiolane-modified poly(ethylene glycol)-block-poly(L-lysine) [PEG-b-(PLL-IM)] and siRNA at a characteristic optimum mixing ratio. The PIC micelles showed a spherical shape of approximately 60 nm in diameter with a narrow distribution. The micellar structure was maintained at physiological ionic strength but was disrupted under reductive conditions because of the cleavage of disulfide cross-links, which is desirable for siRNA release in the intracellular reductive environment. Importantly, environment-responsive PIC micelles achieved 100-fold higher siRNA transfection efficacy compared with non-cross-linked PICs prepared from PEG-b-poly(L-lysine), which were not stable at physiological ionic strength. PICs formed with PEG-b-(PLL-IM) at nonoptimum ratios did not assemble into micellar structure and did not achieve gene silencing following siRNA transfection. These findings show the feasibility of core cross-linked PIC micelles as carriers for therapeutic siRNA and show that stable micellar structure is critical for effective siRNA delivery into target cells.
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Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans
Fire, A., Xu, S., Montgomery, M. K., Kostas, S. A., Driver, S. E., & Mello, C. C. (1998) · Nature · doi:10.1038/35888 · Cited by 15,429
Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells
Elbashir, S. M., Harborth, J., Lendeckel, W., Yalcin, A., Weber, K., & Tuschl, T. (2001) · Nature · doi:10.1038/35078107 · Cited by 9,456
Boussif, O., Lezoualc'h, F., Zanta, M. A., Mergny, M. D., Scherman, D., Demeneix, B., & Behr, J. P. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.16.7297 · Cited by 6,247
Meister, A., & Anderson, M. E. (1983) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.52.070183.003431 · Cited by 5,680
Therapeutic silencing of an endogenous gene by systemic administration of modified siRNAs
Soutschek, J., Akinc, A., Bramlage, B., Charisse, K., Constien, R., Donoghue, M., Elbashir, S., Geick, A., Hadwiger, P., Harborth, J., et al. (2004) · Nature · doi:10.1038/nature03121 · Cited by 2,183
Interfering with disease: a progress report on siRNA-based therapeutics
de Fougerolles, A., Vornlocher, H.-P., Maraganore, J., & Lieberman, J. (2007) · Nature Reviews Drug Discovery · doi:10.1038/nrd2310 · Cited by 1,203
Potent and persistent in vivo anti-HBV activity of chemically modified siRNAs
Morrissey, D. V., Lockridge, J. A., Shaw, L., Blanchard, K., Jensen, K., Breen, W., Hartsough, K., Machemer, L., Radka, S., Jadhav, V., et al. (2005) · Nature Biotechnology · doi:10.1038/nbt1122 · Cited by 1,168
Ellman's reagent: 5,5′-dithiobis(2-nitrobenzoic acid)—a reexamination
Riddles, P. W., Blakeley, R. L., & Zerner, B. (1979) · Analytical Biochemistry · doi:10.1016/0003-2697(79)90792-9 · Cited by 1,015
Schiffelers, R. M. (2004) · Nucleic Acids Research · doi:10.1093/nar/gnh140 · Cited by 901
Harada, A., & Kataoka, K. (1995) · Macromolecules · doi:10.1021/ma00119a019 · Cited by 848
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Liechty, W. B., Kryscio, D. R., Slaughter, B. V., & Peppas, N. A. (2010) · Annual Review of Chemical and Biomolecular Engineering · doi:10.1146/annurev-chembioeng-073009-100847 · Cited by 1,608
Block copolymer micelles in nanomedicine applications
Cabral, H., Miyata, K., Osada, K., & Kataoka, K. (2018) · Chemical Reviews · doi:10.1021/acs.chemrev.8b00199 · Cited by 1,309
Self-citationFleige, E., Quadir, M. A., & Haag, R. (2012) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2012.01.020 · Cited by 1,111
Strategies, design, and chemistry in siRNA delivery systems
Dong, Y., Siegwart, D. J., & Anderson, D. G. (2019) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2019.05.004 · Cited by 572
Talelli, M., Barz, M., Rijcken, C. J. F., Kiessling, F., Hennink, W. E., & Lammers, T. (2015) · Nano Today · doi:10.1016/j.nantod.2015.01.005 · Cited by 506
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Cabane, E., Zhang, X., Langowska, K., Palivan, C. G., & Meier, W. (2012) · Biointerphases · doi:10.1007/s13758-011-0009-3 · Cited by 468
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Jhaveri, A. M., & Torchilin, V. P. (2014) · Frontiers in Pharmacology · doi:10.3389/fphar.2014.00077 · Cited by 466
Sun, H., Guo, B., Cheng, R., Meng, F., Liu, H., & Zhong, Z. (2009) · Biomaterials · doi:10.1016/j.biomaterials.2009.07.051 · Cited by 438
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Li, Y., Lin, T.-Y., Luo, Y., Liu, Q., Xiao, W., Guo, W., Lac, D., Zhang, H., Feng, C., Wachsmann-Hogiu, S., et al. (2014) · Nature Communications · doi:10.1038/ncomms5712 · Cited by 414
Ramasamy, T., Ruttala, H. B., Gupta, B., Poudel, B. K., Choi, H.-G., Yong, C. S., & Kim, J. O. (2017) · Journal of Controlled Release · doi:10.1016/j.jconrel.2017.04.043 · Cited by 373
Polymeric nanostructured materials for biomedical applications
Tang, Z., He, C., Tian, H., Ding, J., Hsiao, B. S., Chu, B., & Chen, X. (2016) · Progress in Polymer Science · doi:10.1016/j.progpolymsci.2016.05.005 · Cited by 363
Stimuli‐Sensitive Synthetic Polypeptide‐Based Materials for Drug and Gene Delivery
He, C., Zhuang, X., Tang, Z., Tian, H., & Chen, X. (2012) · Advanced Healthcare Materials · doi:10.1002/adhm.201100008 · Cited by 337
Osada, K., Christie, R. J., & Kataoka, K. (2009) · Journal of The Royal Society Interface · doi:10.1098/rsif.2008.0547.focus · Cited by 218
Self-citationKe, X., Ng, V. W. L., Ono, R. J., Chan, J. M. W., Krishnamurthy, S., Wang, Y., Hedrick, J. L., & Yang, Y. Y. (2014) · Journal of Controlled Release · doi:10.1016/j.jconrel.2014.06.061 · Cited by 146
Drug-induced morphology switch in drug delivery systems based on poly(2-oxazoline)s
Schulz, A., Jaksch, S., Schubel, R., Wegener, E., Di, Z., Han, Y., Meister, A., Kressler, J., Kabanov, A. V., Luxenhofer, R., et al. (2014) · ACS Nano · doi:10.1021/nn406388t · Cited by 142
Thambi, T., Yoon, H. Y., Kim, K., Kwon, I. C., Yoo, C. K., & Park, J. H. (2011) · Bioconjugate Chemistry · doi:10.1021/bc2000963 · Cited by 138
Smart Nanocarriers for the Delivery of Nucleic Acid‐Based Therapeutics: A Comprehensive Review
Ramasamy, T., Munusamy, S., Ruttala, H. B., & Kim, J. O. (2021) · Biotechnology Journal · doi:10.1002/biot.201900408 · Cited by 76
Polymeric micelles for siRNA delivery
Falamarzian, A., Xiong, X.-B., Uludag, H., & Lavasanifar, A. (2012) · Journal of Drug Delivery Science and Technology · doi:10.1016/s1773-2247(12)50004-3 · Cited by 25
Metadata
- Authors
- Matsumoto, S., Christie, R. J., Nishiyama, N., Miyata, K., Ishii, A., Oba, M., Koyama, H., Yamasaki, Y., & Kataoka, K.
- Venue
- Biomacromolecules
- Publisher
- American Chemical Society (ACS)
- Published
- 2008-12-05
- Volume
- 10
- Issue
- 1
- Pages
- 119-127
- ISSN
- 1525-7797 (ISSN-L)
- 1526-4602
- OpenAlex
- W2015917934
- Cited by
- 327
- References
- 25
- FWCI
- 8.31
- Type
- journal article
- Language
- en
- Open access
- closed
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