Liquid Hydrocarbon Production Potential of a Novel Strain of the Microalga Botryococcus braunii: Assessing the Reliability of in Situ Hydrocarbon Recovery by Wet Process Solvent Extraction
Abstract
Botryococcus braunii is considered a promising source of non-oxygenated liquid biofuels. Harvesting and pre-extraction processing of the algal biomass are among the major challenges for commercial sustainability of algae-based biofuel production. An energy-efficient and cost-effective method of in situ recovery of the hydrocarbon-rich oil from the harvest aqueous culture of B. braunii without pre-extraction processing is an attractive proposition. In this study, in situ recovery of lipid-hydrocarbons by wet process solvent extraction (WPSE) was assessed using the harvest aqueous culture of a novel strain B. braunii GUBIOTJTBB1. The strain yielded 56.3% (w/w) total lipids, 42.6% of which was Soxhlet n -hexane extractable. The recovery of lipid contents was found to vary significantly ( P < 0.001) with the extraction solvent. Fractionation of the total lipids using silica gel column chromatography with n -hexane as eluent yielded lipids (24.9%), which revealed the presence of mostly aliphatic hydrocarbons by GC-MS analysis. In situ recovery rate of the hydrocarbon-rich oil by WPSE, which varies significantly ( P < 0.05) with the method of pretreatments, was the maximum (88.99%) with autoclave-assisted WPSE followed by microwave-assisted WPSE (80%). Applications of multivariate chemometrics in FTIR fingerprint assessment confirmed the reliability of the method. Besides rapidity and minimizing the use of toxic solvents, the principal fate of the method was on the fact that the hydrocarbon-rich oil could be recovered from the harvest aqueous culture, which circumvents the energy intensive pre-extraction processing of the algal biomass.
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Talukdar et al.'s study illustrated the efficiency of high lipid extraction with the support of statistical tools.
“The study illustrated the efficiency of high lipid extraction with the support of statistical tools.”
From Factors Affecting Microalgae Production for Biofuels and the Potentials of Chemometric Methods in Assessing and Optimizing Productivity (2019) · cites this paper as [109]
In Talukdar et al.'s study, PCA revealed the most prominent variation patterns.
“The PCA revealed the most prominent variation patterns.”
From Factors Affecting Microalgae Production for Biofuels and the Potentials of Chemometric Methods in Assessing and Optimizing Productivity (2019) · cites this paper as [109]
In Talukdar et al.'s study, the first two PCs accounted for 94.34% of variability in the data set.
“The first two PCs accounted for 94.34% of variability in the data set.”
From Factors Affecting Microalgae Production for Biofuels and the Potentials of Chemometric Methods in Assessing and Optimizing Productivity (2019) · cites this paper as [109]
In Talukdar et al.'s study, ATR-FTIR combined with chemometrics revealed related spectra patterns in the data set.
“In this study ATR-FTIR combined with chemometrics revealed related spectra patterns in the data set.”
From Factors Affecting Microalgae Production for Biofuels and the Potentials of Chemometric Methods in Assessing and Optimizing Productivity (2019) · cites this paper as [109]
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- Authors
- Talukdar, J., Kalita, M. C., Goswami, B. C., Hong, D. D., & Das, H. C.
- Affiliations
- Gauhati University (IN)
- Institute of Biotechnology, Vietnamese Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay 1000, Hanoi, Vietnam
- Oil India (India) (IN)
- Corresponding author
- Talukdar, Jayanta
- Venue
- Energy & Fuels
- Publisher
- American Chemical Society (ACS)
- Published
- 2014-05-08
- Volume
- 28
- Issue
- 6
- Pages
- 3747-3758
- ISSN
- 0887-0624 (ISSN-L)
- 1520-5029
- OpenAlex
- W2326078377
- Cited by
- 18
- References
- 38
- Citations by year
- 2026: 3 · 2025: 2 · 2024: 1 · 2022: 1 · 2021: 1 · 2020: 2 · 2019: 1 · 2018: 2 · 2017: 2 · 2016: 2 · 2015: 1
- FWCI
- 0.55
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Energy & Fuels (publishedVersion)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Keywords
- Botryococcus braunii, microalgae, liquid hydrocarbon production, aliphatic hydrocarbons, microwave-assisted extraction, algal biofuels
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