Infrared Spectroscopy: Fundamentals and Applications
Abstract
Provides an introduction to those needing to use infrared spectroscopy for the first time, explaining the fundamental aspects of this technique, how to obtain a spectrum and how to analyse infrared data covering a wide range of applications. Includes instrumental and sampling techniques Covers biological and industrial applications Includes suitable questions and problems in each chapter to assist in the analysis and interpretation of representative infrared spectra Part of the ANTS (Analytical Techniques in the Sciences) Series.
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Nearly 80% of the TAGs are saturated and monounsaturated fatty acids.
“Studies have shown that nearly 80% of the TAGs are saturated (SFA) and monounsaturated fatty acids (MUFA)[3,52,61,62], and their vibration can be attributed to the carbonyl stretches of esters in the fatty acids at ~1740 cm -1 .”
From Response to nutrient variation on lipid productivity in green microalgae captured using second derivative FTIR and Raman spectroscopy (2022) · cites this paper as [52]
SD-FTIR and SD-Raman spectra revealed the presence of all four characteristic stretches of hydrocarbons (νasCH3, νasCH2, νsCH3, νsCH2) in the expected region 3000-2800 cm-1.
“As SD spectra enhance the spectral resolution, the presence of all the four characteristic stretches of hydrocarbons ( ν asCH3 , ν asCH2 , ν sCH3 , ν sCH2) was revealed by the SD-FTIR (Fig. 1B) and SD-Raman spectra (Fig. 1D) in the expected region 3000 -2800 cm -1 [23,52,54].”
From Response to nutrient variation on lipid productivity in green microalgae captured using second derivative FTIR and Raman spectroscopy (2022) · cites this paper as [52]
Biomolecular groups in Chlamydomonas sp. were identified and variation in peak intensity between NP treatments and days of growth was assessed.
“Similarly, for Chlamydomonas sp ., (Fig.3 and 4) their biomolecular groups were identified and the variation in the intensity of the peaks between NP treatments and the days of growth was assessed and is tabulated in Table 3 [9,20,23,52 -54].”
From Response to nutrient variation on lipid productivity in green microalgae captured using second derivative FTIR and Raman spectroscopy (2022) · cites this paper as [52]
The ~2850 cm−1 IR band is assigned to νs(CH2) symmetric stretches.
“~ 2846 | ~ 2820 | s + | m + | s + | w + | s + | m + | vs + |”
From Response to nutrient variation on lipid productivity in green microalgae captured using second derivative FTIR and Raman spectroscopy (2022) · cites this paper as [52]
The ~3010 cm−1 IR band is assigned to (=CH) stretches from unsaturated lipids.
“~ 3010 | ~ 3014 | w + | w + | w + | vw + | w + | vw + | w + |”
From Response to nutrient variation on lipid productivity in green microalgae captured using second derivative FTIR and Raman spectroscopy (2022) · cites this paper as [52]
The ~1740 cm−1 IR band is assigned to ν(C=O) stretches of esters from lipids - Triacylglycerides (TAGs).
“~ 1740 | | m + | | m + | | m + | | s + |”
From Response to nutrient variation on lipid productivity in green microalgae captured using second derivative FTIR and Raman spectroscopy (2022) · cites this paper as [52]
The ~2924 cm−1 IR band is assigned to (CH2) asymmetric stretches from hydrocarbons.
“~ 2924 | ~ 2912 | s + | vw + | m + | vw + | m + | vw + | s + |”
From Response to nutrient variation on lipid productivity in green microalgae captured using second derivative FTIR and Raman spectroscopy (2022) · cites this paper as [52]
The ~1654 cm−1 IR band is assigned to ν(C=O), ν(C-N) stretches and δ(N-H) deformations of Amide I from proteins.
“~ 1654 | ~ 1658 | s + | w + | s + | vw + | s + | vw + | m + |”
From Response to nutrient variation on lipid productivity in green microalgae captured using second derivative FTIR and Raman spectroscopy (2022) · cites this paper as [52]
The ~1440 cm−1 Raman band is assigned to δ(CH2) scissoring from saturated lipids and hydrocarbons.
“| ~ 1440 | | s + | | s + | | s + |”
From Response to nutrient variation on lipid productivity in green microalgae captured using second derivative FTIR and Raman spectroscopy (2022) · cites this paper as [52]
The ~1658 cm−1 Raman band is assigned to cis ν(C=C) from unsaturated lipids.
“~ 1654 | ~ 1658 | s + | w + | s + | vw + | s + | vw + | m + |”
From Response to nutrient variation on lipid productivity in green microalgae captured using second derivative FTIR and Raman spectroscopy (2022) · cites this paper as [52]
The ~1266 cm−1 IR band is assigned to δ(=CH) deformations from unsaturated lipids.
“~ 1266 | ~ 1264 | vw + | w + | vw + | w + | vw + | w + | vw + |”
From Response to nutrient variation on lipid productivity in green microalgae captured using second derivative FTIR and Raman spectroscopy (2022) · cites this paper as [52]
The ~1380 cm−1 IR band is assigned to δs(CH3) symmetric bend from lipids and proteins.
“~ 1380 | | vs - | | s - | | m - | | w - |”
From Response to nutrient variation on lipid productivity in green microalgae captured using second derivative FTIR and Raman spectroscopy (2022) · cites this paper as [52]
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- Authors
- Stuart, B. H.
- Corresponding author
- Stuart, Barbara H.
- ORCID
- Stuart, Barbara H.
- Venue
- Analytical Techniques in the Sciences
- Publisher
- Wiley
- Published
- 2004-06-25
- ISSN
- 1935-0732 (ISSN-L)
- 1935-0740
- OpenAlex
- W639767525
- Cited by
- 4,427
- References
- 21
- Citations by year
- 2026: 247 · 2025: 296 · 2024: 314 · 2023: 272 · 2022: 280 · 2021: 266 · 2020: 287 · 2019: 299 · 2018: 298 · 2017: 293 · 2016: 293 · 2015: 305 · 2014: 220 · 2013: 173 · 2012: 150
- FWCI
- 10.52
- Type
- book
- Language
- en
- Open access
- closed
- Locations
- Analytical techniques in the sciences (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Spectroscopy Techniques in Biomedical and Chemical Research
- Physical Sciences › Chemistry › Analytical Chemistry › Spectroscopy and Chemometric Analyses
- Physical Sciences › Chemistry › Spectroscopy › Spectroscopy and Laser Applications
- Keywords
- infrared spectroscopy, infrared spectrum interpretation, infrared instrumentation, biological applications, industrial applications
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