The DOI matches its Crossref record; 11 of the 11 references it cites were found in a registry or an index.
Ultrasensitive DNA Detection Using Highly Fluorescent Bioconjugated Nanoparticles
Abstract
Sensitive DNA detection is extremely important in clinical diagnostics, gene therapy, and a variety of biomedical studies. We have developed a novel DNA bioanalysis method with a 0.8 fM (0.8 x 10-15 M) detection limit using a bioconjugated fluorescent nanoparticle-based sandwich assay. An organic dye silica nanoparticle is synthesized using a modified reverse microemulsion. The nanoparticles are highly fluorescent, extremely photostable, and easy for bioconjugation for bioanalysis. They exhibit an excellent signaling ability in the presence of trace amounts of DNA targets. With an effective surface modification, nonspecific binding and nanoparticle aggregation are minimized. In addition, the nanoparticle-based DNA bioanalysis assay can effectively discriminate one-base mismatched DNA sequences. We expect this nanoparticle-based assay to be widely useful in a number of biomedical applications where reproducible, selective, and ultrasensitive gene analysis is critical.
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Cites
Quantum Dot Bioconjugates for Ultrasensitive Nonisotopic Detection
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Nanoparticles with Raman Spectroscopic Fingerprints for DNA and RNA Detection
Cao, Y. C., Jin, R., & Mirkin, C. A. (2002) · Science · doi:10.1126/science.297.5586.1536 · Cited by 2,971
Scanometric DNA Array Detection with Nanoparticle Probes
Taton, T. A., Mirkin, C. A., & Letsinger, R. L. (2000) · Science · doi:10.1126/science.289.5485.1757 · Cited by 2,411
Fluorescence Spectroscopy of Single Biomolecules
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A Group-IV Ferromagnetic Semiconductor: Mn x Ge 1− x
Park, Y. D., Hanbicki, A. T., Erwin, S. C., Hellberg, C. S., Sullivan, J. M., Mattson, J. E., Ambrose, T. F., Wilson, A., Spanos, G., & Jonker, B. T. (2002) · Science · doi:10.1126/science.1066348 · Cited by 1,631
Colloidal Au-Enhanced Surface Plasmon Resonance for Ultrasensitive Detection of DNA Hybridization
He, L., Musick, M. D., Nicewarner, S. R., Salinas, F. G., Benkovic, S. J., Natan, M. J., & Keating, C. D. (2000) · Journal of the American Chemical Society · doi:10.1021/ja001215b · Cited by 932
Self-Assembled Nanoparticle Probes for Recognition and Detection of Biomolecules
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Conjugation of Biomolecules with Luminophore-Doped Silica Nanoparticles for Photostable Biomarkers
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Self-citationMagnetically-Induced Solid-State Electrochemical Detection of DNA Hybridization
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Designing a Novel Molecular Beacon for Surface-Immobilized DNA Hybridization Studies
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Self-citation
Cited by
Nanostructures in Biodiagnostics
Rosi, N. L., & Mirkin, C. A. (2005) · Chemical Reviews · doi:10.1021/cr030067f · Cited by 4,603
Cancer nanotechnology: opportunities and challenges
Ferrari, M. (2005) · Nature Reviews Cancer · doi:10.1038/nrc1566 · Cited by 4,558
DNA Biosensors and Microarrays
Sassolas, A., Leca-Bouvier, B. D., & Blum, L. J. (2008) · Chemical Reviews · doi:10.1021/cr0684467 · Cited by 1,246
Nyk, M., Kumar, R., Ohulchanskyy, T. Y., Bergey, E. J., & Prasad, P. N. (2008) · Nano Letters · doi:10.1021/nl802223f · Cited by 905
Surface Modification of Silica Nanoparticles to Reduce Aggregation and Nonspecific Binding
Bagwe, R. P., Hilliard, L. R., & Tan, W. (2006) · Langmuir · doi:10.1021/la052797j · Cited by 870
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Metadata
- Authors
- Zhao, X., Tapec-Dytioco, R., & Tan, W.
- Venue
- Journal of the American Chemical Society
- Publisher
- American Chemical Society (ACS)
- Published
- 2003-08-30
- Volume
- 125
- Issue
- 38
- Pages
- 11474-11475
- ISSN
- 0002-7863 (ISSN-L)
- 1520-5126
- OpenAlex
- W2050092543
- Cited by
- 548
- References
- 11
- FWCI
- 7.94
- Type
- journal article
- Language
- en
- Open access
- closed
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