Cited by 132Advanced Proteomics Techniques and ApplicationsMass Spectrometry Techniques and ApplicationsMetabolomics and Mass Spectrometry Studies

The Revolution and Evolution of Shotgun Proteomics for Large-Scale Proteome Analysis

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Yates, John R.·2013·Journal of the American Chemical Society

Abstract

Mass spectrometry has evolved at an exponential rate over the last 100 years. Innovations in the development of mass spectrometers have created powerful instruments capable of analyzing a wide range of targets, from rare atoms and molecules to very large molecules, such as a proteins, protein complexes, and DNA. These performance gains have been driven by sustaining innovations, punctuated by the occasional disruptive innovation. The use of mass spectrometry for proteome analysis was driven by disruptive innovations that created a capability for large-scale analysis of proteins and modifications.

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Authors
Yates, J. R.
Affiliations
Scripps Research Institute (US)
Corresponding author
Yates, John R.
Venue
Journal of the American Chemical Society
Published
2013-01-16
Volume
135
Issue
5
Pages
1629-1640
ISSN
  • 0002-7863 (ISSN-L)
  • 1520-5126
OpenAlex
W35392279
Cited by
131
References
145
Citations by year
2026: 4 · 2025: 7 · 2024: 10 · 2023: 4 · 2022: 8 · 2021: 7 · 2020: 6 · 2019: 12 · 2018: 11 · 2017: 13 · 2016: 12 · 2015: 13 · 2014: 14 · 2013: 9
FWCI
7.19
Type
journal article
Language
en
Open access
Locations
  • Journal of the American Chemical Society (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • U.S. Public Health Service (HHSN2682010000035C)
  • National Institute on Aging (P01AG031097)
  • National Heart, Lung, and Blood Institute (R01HL079442)
  • National Institute of Mental Health (R01MH067880)
  • National Institute of General Medical Sciences (P41GM103533, P41 RR011823/GM103533)
  • National Center for Research Resources (P41RR011823)
Topics
  • Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
  • Physical Sciences › Chemistry › Spectroscopy › Mass Spectrometry Techniques and Applications
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
Keywords
shotgun proteomics, mass spectrometry instrumentation, disruptive innovation, sustained innovation, protein modification, protein complexes, history of mass spectrometry
MeSH terms
Animals, Humans, Models, Molecular, Mass Spectrometry, Proteome, Proteomics, Mice, Rats

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