The DOI matches its Crossref record; 17 of the 17 references it cites were found in a registry or an index.
Full-Range Intracellular pH Sensing by an Aggregation-Induced Emission-Active Two-Channel Ratiometric Fluorogen
Abstract
Intracellular pH (pHi) is an important parameter associated with cellular behaviors and pathological conditions. Sensing pHi and monitoring its changes in live cells are essential but challenging due to the lack of effective probes. We herein report a pH-sensitive fluorogen for pHi sensing and tracking. The dye is a tetraphenylethene-cyanine adduct (TPE-Cy). It is biocompatible and cell-permeable. Upon diffusing into cells, it responds sensitively to pHi in the entire physiological range, visualizing the acidic and basic compartments with intense red and blue emissions, respectively. The ratiometric signal of the red and blue channels can thus serve as an indicator for local proton concentration. The utility of TPE-Cy in pHi imaging and monitoring is demonstrated with the use of confocal microscopy, ratiometric analysis, and flow cytometry.
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Cites
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Cited by
Aggregation-Induced Emission: Together We Shine, United We Soar!
Mei, J., Leung, N. L. C., Kwok, R. T. K., Lam, J. W. Y., & Tang, B. Z. (2015) · Chemical Reviews · doi:10.1021/acs.chemrev.5b00263 · Cited by 8,270
Self-citationAggregation‐Induced Emission: The Whole Is More Brilliant than the Parts
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Self-citationOptical Sensing and Imaging of pH Values: Spectroscopies, Materials, and Applications
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Aggregation-Induced Emission Luminogens for Activity-Based Sensing
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Self-citationProgress and Trends in AIE-Based Bioprobes: A Brief Overview
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Metadata
- Authors
- Chen, S., Hong, Y., Liu, Y., Liu, J., Leung, C. W. T., Li, M., Kwok, R. T. K., Zhao, E., Lam, J. W. Y., Yu, Y., & Tang, B. Z.
- Venue
- Journal of the American Chemical Society
- Publisher
- American Chemical Society (ACS)
- Published
- 2013-03-26
- Volume
- 135
- Issue
- 13
- Pages
- 4926-4929
- ISSN
- 0002-7863 (ISSN-L)
- 1520-5126
- OpenAlex
- W2026227022
- Cited by
- 434
- References
- 17
- FWCI
- 22.41
- Type
- journal article
- Language
- en
- Open access
- closed
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