The DOI matches its Crossref record; 52 of the 52 references it cites were found in a registry or an index.
A Ratiometric Two-Photon Fluorescent Probe Reveals Reduction in Mitochondrial H2S Production in Parkinson’s Disease Gene Knockout Astrocytes
Abstract
Hydrogen sulfide (H2S) is a multifunctional signaling molecule that exerts neuroprotective effects in oxidative stress. In this article, we report a mitochondria-localized two-photon probe, SHS-M2, that can be excited by 750 nm femtosecond pulses and employed for ratiometric detection of H2S in live astrocytes and living brain slices using two-photon microscopy (TPM). SHS-M2 shows bright two-photon-excited fluorescence and a marked change in emission color from blue to yellow in response to H2S, low cytotoxicity, easy loading, and minimum interference from other biologically relevant species including reactive sulfur, oxygen, and nitrogen species, thereby allowing quantitative analysis of H2S levels. Molecular TPM imaging with SHS-M2 in astrocytes revealed that there is a correlation between the ratiometric analysis and expression levels of cystathionine β-synthase (CBS), the major enzyme that catalyzes H2S production. In studies involving DJ-1, a Parkinson's disease (PD) gene, attenuated H2S production in comparison with wild-type controls was observed in DJ-1-knockout astrocytes and brain slices, where CBS expression was decreased. These findings demonstrate that reduced H2S levels in astrocytes may contribute to the development of PD and that SHS-M2 may be useful as a marker to detect a risk of neurodegenerative diseases, including PD.
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Cites
1 retracted
Brain hydrogen sulfide is severely decreased in Alzheimer's disease
Eto, K., Asada, T., Arima, K., Makifuchi, T., & Kimura, H. (2002) · Biochemical and Biophysical Research Communications · doi:10.1016/s0006-291x(02)00422-9 · Cited by 809
RetractedMeasurement of photoluminescence quantum yields. Review
Crosby, G. A., & Demas, J. N. (1971) · The Journal of Physical Chemistry · doi:10.1021/j100678a001 · Cited by 4,870
Deep tissue two-photon microscopy
Helmchen, F., & Denk, W. (2005) · Nature Methods · doi:10.1038/nmeth818 · Cited by 4,153
Nonlinear magic: multiphoton microscopy in the biosciences
Zipfel, W. R., Williams, R. M., & Webb, W. W. (2003) · Nature Biotechnology · doi:10.1038/nbt899 · Cited by 3,893
A simple method for organotypic cultures of nervous tissue
Stoppini, L., Buchs, P.-A., & Muller, D. (1991) · Journal of Neuroscience Methods · doi:10.1016/0165-0270(91)90128-m · Cited by 3,089
The possible role of hydrogen sulfide as an endogenous neuromodulator
Abe, K., & Kimura, H. (1996) · The Journal of Neuroscience · doi:10.1523/jneurosci.16-03-01066.1996 · Cited by 2,239
Hosoki, R., Matsuki, N., & Kimura, H. (1997) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.1997.6878 · Cited by 1,218
Mitochondrial Glutathione, a Key Survival Antioxidant
Marí, M., Morales, A., Colell, A., García-Ruiz, C., & Fernández-Checa, J. C. (2009) · Antioxidants & Redox Signaling · doi:10.1089/ars.2009.2695 · Cited by 980
3-Mercaptopyruvate Sulfurtransferase Produces Hydrogen Sulfide and Bound Sulfane Sulfur in the Brain
Shibuya, N., Tanaka, M., Yoshida, M., Ogasawara, Y., Togawa, T., Ishii, K., & Kimura, H. (2009) · Antioxidants & Redox Signaling · doi:10.1089/ars.2008.2253 · Cited by 919
Two-photon absorption standards in the 550-1600 nm excitation wavelength range
Makarov, N. S., Drobizhev, M., & Rebane, A. (2008) · Optics Express · doi:10.1364/oe.16.004029 · Cited by 894
Hydrogen sulfide is an endogenous modulator of leukocyte‐mediated inflammation
Zanardo, R. C. O., Brancaleone, V., Distrutti, E., Fiorucci, S., Cirino, G., Wallace, J. L., Zanardo, R. C. O., Brancaleone, V., Distrutti, E., Fiorucci, S., et al. (2006) · The FASEB Journal · doi:10.1096/fj.06-6270fje · Cited by 859
Reaction-Based Fluorescent Probes for Selective Imaging of Hydrogen Sulfide in Living Cells
Lippert, A. R., New, E. J., & Chang, C. J. (2011) · Journal of the American Chemical Society · doi:10.1021/ja203661j · Cited by 760
Redox Biochemistry of Hydrogen Sulfide
Kabil, O., & Banerjee, R. (2010) · Journal of Biological Chemistry · doi:10.1074/jbc.r110.128363 · Cited by 744
Manders, E. M. M., Stap, J., Brakenhoff, G. J., Driel, R. V., & Aten, J. A. (1992) · Journal of Cell Science · doi:10.1242/jcs.103.3.857 · Cited by 739
Hydrogen Sulfide Increases Glutathione Production and Suppresses Oxidative Stress in Mitochondria
Kimura, Y., Goto, Y.-I., & Kimura, H. (2010) · Antioxidants & Redox Signaling · doi:10.1089/ars.2008.2282 · Cited by 685
Development of a Highly Selective Fluorescence Probe for Hydrogen Sulfide
Sasakura, K., Hanaoka, K., Shibuya, N., Mikami, Y., Kimura, Y., Komatsu, T., Ueno, T., Terai, T., Kimura, H., & Nagano, T. (2011) · Journal of the American Chemical Society · doi:10.1021/ja207851s · Cited by 661
A Fluorescent Probe for Fast and Quantitative Detection of Hydrogen Sulfide in Blood
Peng, H., Cheng, Y., Dai, C., King, A. L., Predmore, B. L., Lefer, D. J., & Wang, B. (2011) · Angewandte Chemie International Edition · doi:10.1002/anie.201104236 · Cited by 637
Hydrogen sulfide: its production, release and functions
Kimura, H. (2011) · Amino Acids · doi:10.1007/s00726-010-0510-x · Cited by 634
Singh, S., Padovani, D., Leslie, R. A., Chiku, T., & Banerjee, R. (2009) · Journal of Biological Chemistry · doi:10.1074/jbc.m109.010868 · Cited by 614
Capture and Visualization of Hydrogen Sulfide by a Fluorescent Probe
Liu, C., Pan, J., Li, S., Zhao, Y., Wu, L. Y., Berkman, C. E., Whorton, A. R., & Xian, M. (2011) · Angewandte Chemie International Edition · doi:10.1002/anie.201104305 · Cited by 578
A Ratiometric Fluorescent Probe for Rapid Detection of Hydrogen Sulfide in Mitochondria
Chen, Y., Zhu, C., Yang, Z., Chen, J., He, Y., Jiao, Y., He, W., Qiu, L., Cen, J., & Guo, Z. (2013) · Angewandte Chemie International Edition · doi:10.1002/anie.201207701 · Cited by 563
Kim, H. M., & Cho, B. R. (2009) · Accounts of Chemical Research · doi:10.1021/ar800185u · Cited by 542
Self-citationSelective fluorescent probes for live-cell monitoring of sulphide
Qian, Y., Karpus, J., Kabil, O., Zhang, S.-Y., Zhu, H.-L., Banerjee, R., Zhao, J., & He, C. (2011) · Nature Communications · doi:10.1038/ncomms1506 · Cited by 506
Hydrogen sulfide (H 2 S) metabolism in mitochondria and its regulatory role in energy production
Fu, M., Zhang, W., Wu, L., Yang, G., Li, H., & Wang, R. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1115634109 · Cited by 472
Growth retardation and cysteine deficiency in gamma-glutamyl transpeptidase-deficient mice.
Lieberman, M. W., Wiseman, A. L., Shi, Z. Z., Carter, B. Z., Barrios, R., Ou, C. N., Chévez-Barrios, P., Wang, Y., Habib, G. M., Goodman, J. C., et al. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.15.7923 · Cited by 374
Neuroprotective effects of hydrogen sulfide on Parkinson’s disease rat models
Hu, L., Lu, M., Tiong, C. X., Dawe, G. S., Hu, G., & Bian, J. (2010) · Aging Cell · doi:10.1111/j.1474-9726.2009.00543.x · Cited by 365
Ratiometric Detection of Mitochondrial Thiols with a Two-Photon Fluorescent Probe
Lim, C. S., Masanta, G., Kim, H. J., Han, J. H., Kim, H. M., & Cho, B. R. (2011) · Journal of the American Chemical Society · doi:10.1021/ja205081s · Cited by 365
Self-citationAcute hydrogen sulfide poisoning
Warenycia, M. W., Goodwin, L. R., Benishin, C. G., Reiffenstein, R. J., Francom, D. M., Taylor, J. D., & Dieken, F. P. (1989) · Biochemical Pharmacology · doi:10.1016/0006-2952(89)90288-8 · Cited by 329
Goodwin, L. R., Francom, D., Dieken, F. P., Taylor, J. D., Warenycia, M. W., Reiffenstein, R. J., & Dowling, G. (1989) · Journal of Analytical Toxicology · doi:10.1093/jat/13.2.105 · Cited by 323
Interaction of DJ-1 with Daxx inhibits apoptosis signal-regulating kinase 1 activity and cell death
Junn, E., Taniguchi, H., Jeong, B. S., Zhao, X., Ichijo, H., & Mouradian, M. M. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0409635102 · Cited by 321
Reaction-Based Genetically Encoded Fluorescent Hydrogen Sulfide Sensors
Chen, S., Chen, Z.-J., Ren, W., & Ai, H.-W. (2012) · Journal of the American Chemical Society · doi:10.1021/ja303261d · Cited by 318
Two‐Photon Fluorescent Probes for Bioimaging
Yao, S., & Belfield, K. D. (2012) · European Journal of Organic Chemistry · doi:10.1002/ejoc.201200281 · Cited by 261
Age-dependent Motor Deficits and Dopaminergic Dysfunction in DJ-1 Null Mice
Chen, L., Cagniard, B., Mathews, T., Jones, S., Koh, H. C., Ding, Y., Carvey, P. M., Ling, Z., Kang, U. J., & Zhuang, X. (2005) · Journal of Biological Chemistry · doi:10.1074/jbc.m413955200 · Cited by 261
A Mitochondrial-Targeted Two-Photon Probe for Zinc Ion
Masanta, G., Lim, C. S., Kim, H. J., Han, J. H., Kim, H. M., & Cho, B. R. (2011) · Journal of the American Chemical Society · doi:10.1021/ja200444t · Cited by 243
Self-citationPyo, H., Jou, I., Jung, S., Hong, S., & Joe, E.-H. (1998) · NeuroReport · doi:10.1097/00001756-199803300-00020 · Cited by 217
Self-citationAstrocytes produce the antiinflammatory and neuroprotective agent hydrogen sulfide
Lee, M., Schwab, C., Yu, S., McGeer, E., & McGeer, P. L. (2009) · Neurobiology of Aging · doi:10.1016/j.neurobiolaging.2009.06.001 · Cited by 203
2,6-Bis[4-(p-dihexylaminostyryl)styryl]anthracene Derivatives with Large Two-Photon Cross Sections
Lee, S. K., Yang, W. J., Choi, J. J., Kim, C. H., Jeon, S.-J., & Cho, B. R. (2005) · Organic Letters · doi:10.1021/ol047658s · Cited by 198
Self-citationRegulation of astrocyte inflammatory responses by the Parkinson's disease‐associated gene DJ–1
Waak, J., Weber, S. S., Waldenmaier, A., Görner, K., Alunni‐Fabbroni, M., Schell, H., Vogt‐Weisenhorn, D., Pham, T., Reumers, V., Baekelandt, V., et al. (2009) · The FASEB Journal · doi:10.1096/fj.08-125153 · Cited by 186
Li, L., Ge, J., Wu, H., Xu, Q.-H., & Yao, S. Q. (2012) · Journal of the American Chemical Society · doi:10.1021/ja3036256 · Cited by 172
Metal-Ion-Responsive Fluorescent Probes for Two-Photon Excitation Microscopy
Sumalekshmy, S., & Fahrni, C. J. (2011) · Chemistry of Materials · doi:10.1021/cm1021905 · Cited by 169
Masanta, G., Heo, C. H., Lim, C. S., Bae, S. K., Cho, B. R., & Kim, H. M. (2012) · Chemical Communications · doi:10.1039/c2cc00034b · Cited by 158
Self-citationGriffith, O. W., Bridges, R. J., & Meister, A. (1978) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.75.11.5405 · Cited by 141
A turn-on two-photon fluorescent probe for ATP and ADP
Rao, A. S., Kim, D., Nam, H., Jo, H., Kim, K. H., Ban, C., & Ahn, K. H. (2012) · Chemical Communications · doi:10.1039/c2cc17629g · Cited by 133
Two‐Photon Fluorescent Probes for Metal Ions
Kim, H. M., & Cho, B. R. (2010) · Chemistry - An Asian Journal · doi:10.1002/asia.201000542 · Cited by 132
Self-citationReproductive Defects in γ-Glutamyl Transpeptidase-Deficient Mice1
Kumar, T. R., Wiseman, A. L., Kala, G., Kala, S. V., Matzuk, M. M., & Lieberman, M. W. (2000) · Endocrinology · doi:10.1210/endo.141.11.7760 · Cited by 90
Oxidized DJ-1 Interacts with the Mitochondrial Protein BCL-XL
Ren, H., Fu, K., Wang, D., Mu, C., & Wang, G. (2011) · Journal of Biological Chemistry · doi:10.1074/jbc.m110.207134 · Cited by 80
Plasminogen-induced IL-1β and TNF-α production in microglia is regulated by reactive oxygen species
Min, K., Jou, I., & Joe, E.-H. (2003) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2003.11.010 · Cited by 70
Self-citationPun, P. B. L., Lu, J., Kan, E. M., & Moochhala, S. (2009) · Mitochondrion · doi:10.1016/j.mito.2009.12.142 · Cited by 58
Cheng, W.-Y., Tong, H., Miller, E. W., Chang, C. J., Remington, S. J., Zucker, R. M., Bromberg, P. A., Samet, J. M., & Höfer, T. (2010) · Environmental Health Perspectives · doi:10.1289/ehp.0901811 · Cited by 57
Huuskonen, J., Suuronen, T., Miettinen, R. A., van Groen, T., & Salminen, A. (2005) · Journal of Neuroinflammation · doi:10.1186/1742-2094-2-25 · Cited by 57
Two-photon excited fluorescent probes for calcium based on internal charge transfer
Dong, X., Yang, Y., Sun, J., Liu, Z., & Liu, B. (2009) · Chemical Communications · doi:10.1039/b905571a · Cited by 50
Parkinson’s disease-associated DJ-1 modulates innate immunity signaling in Caenorhabditis elegans
Castro, E. M. C., Waak, J., Weber, S. S., Fiesel, F. C., Oberhettinger, P., Schütz, M. S., Autenrieth, I. B., Springer, W., & Kahle, P. J. (2010) · Journal of Neural Transmission · doi:10.1007/s00702-010-0397-4 · Cited by 32
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Zielonka, J., Joseph, J., Sikora, A., Hardy, M., Ouari, O., Vasquez-Vivar, J., Cheng, G., Lopez, M., & Kalyanaraman, B. (2017) · Chemical Reviews · doi:10.1021/acs.chemrev.7b00042 · Cited by 1,683
Small molecule-based ratiometric fluorescence probes for cations, anions, and biomolecules
Lee, M. H., Kim, J. S., & Sessler, J. L. (2015) · Chemical Society Reviews · doi:10.1039/c4cs00280f · Cited by 1,642
Small-Molecule Two-Photon Probes for Bioimaging Applications
Kim, H. M., & Cho, B. R. (2015) · Chemical Reviews · doi:10.1021/cr5004425 · Cited by 1,047
Self-citationFluorescent Probes for Disease Diagnosis
Wang, X., Ding, Q., Groleau, R. R., Wu, L., Mao, Y., Che, F., Kotova, O., Scanlan, E. M., Lewis, S. E., Li, P., et al. (2024) · Chemical Reviews · doi:10.1021/acs.chemrev.3c00776 · Cited by 543
Two-photon luminescent metal complexes for bioimaging and cancer phototherapy
Chen, Y., Guan, R., Zhang, C., Huang, J., Ji, L., & Chao, H. (2016) · Coordination Chemistry Reviews · doi:10.1016/j.ccr.2015.09.010 · Cited by 259
Metadata
- Authors
- Bae, S. K., Heo, C. H., Choi, D. J., Sen, D., Joe, E.-H., Cho, B. R., & Kim, H. M.
- Venue
- Journal of the American Chemical Society
- Publisher
- American Chemical Society (ACS)
- Published
- 2013-06-18
- Volume
- 135
- Issue
- 26
- Pages
- 9915-9923
- ISSN
- 0002-7863 (ISSN-L)
- 1520-5126
- OpenAlex
- W2071919400
- Cited by
- 411
- References
- 52
- FWCI
- 23.18
- Type
- journal article
- Language
- en
- Open access
- closed
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