The DOI matches its Crossref record; 44 of the 44 references it cites were found in a registry or an index.
Fluorescent Probe HKSOX-1 for Imaging and Detection of Endogenous Superoxide in Live Cells and In Vivo
Abstract
Superoxide anion radical (O2(•-)) is undoubtedly the most important primary reactive oxygen species (ROS) found in cells, whose formation and fate are intertwined with diverse physiological and pathological processes. Here we report a highly sensitive and selective O2(•-) detecting strategy involving O2(•-) cleavage of an aryl trifluoromethanesulfonate group to yield a free phenol. We have synthesized three new O2(•-) fluorescent probes (HKSOX-1, HKSOX-1r for cellular retention, and HKSOX-1m for mitochondria-targeting) which exhibit excellent selectivity and sensitivity toward O2(•-) over a broad range of pH, strong oxidants, and abundant reductants found in cells. In confocal imaging, flow cytometry, and 96-well microplate assay, HKSOX-1r has been robustly applied to detect O2(•-) in multiple cellular models, such as inflammation and mitochondrial stress. Additionally, our probes can be efficiently applied to visualize O2(•-) in intact live zebrafish embryos. These probes open up exciting opportunities for unmasking the roles of O2(•-) in health and disease.
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Defining roles of specific reactive oxygen species (ROS) in cell biology and physiology
Sies, H., Belousov, V. V., Chandel, N. S., Davies, M. J., Jones, D. P., Mann, G. E., Murphy, M. P., Yamamoto, M., & Winterbourn, C. (2022) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-022-00456-z · Cited by 1,735
Zielonka, J., Joseph, J., Sikora, A., Hardy, M., Ouari, O., Vasquez-Vivar, J., Cheng, G., Lopez, M., & Kalyanaraman, B. (2017) · Chemical Reviews · doi:10.1021/acs.chemrev.7b00042 · Cited by 1,683
Molecular optical imaging probes for early diagnosis of drug-induced acute kidney injury
Huang, J., Li, J., Lyu, Y., Miao, Q., & Pu, K. (2019) · Nature Materials · doi:10.1038/s41563-019-0378-4 · Cited by 721
Nitroxidative signaling mechanisms in pathological pain
Grace, P. M., Gaudet, A. D., Staikopoulos, V., Maier, S. F., Hutchinson, M. R., Salvemini, D., & Watkins, L. R. (2016) · Trends in Neurosciences · doi:10.1016/j.tins.2016.10.003 · Cited by 128
Gao, X., Feng, G., Manghnani, P. N., Hu, F., Jiang, N., Liu, J., Liu, B., Sun, J. Z., & Tang, B. Z. (2017) · Chemical Communications · doi:10.1039/c6cc09307h · Cited by 121
Metadata
- Authors
- Hu, J. J., Wong, N.-K., Ye, S., Chen, X., Lu, M.-Y., Zhao, A. Q., Guo, Y., Ma, A. C.-H., Leung, A. Y.-H., Shen, J., & Yang, D.
- Venue
- Journal of the American Chemical Society
- Publisher
- American Chemical Society (ACS)
- Published
- 2015-05-19
- Volume
- 137
- Issue
- 21
- Pages
- 6837-6843
- ISSN
- 0002-7863 (ISSN-L)
- 1520-5126
- OpenAlex
- W1159415896
- Cited by
- 279
- References
- 44
- FWCI
- 13.87
- Type
- journal article
- Language
- en
- Open access
- closed
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- Hu, J. J., Wong, N.-K., Ye, S., Chen, X., Lu, M.-Y., Zhao, A. Q., Guo, Y., Ma, A. C.-H., Leung, A. Y.-H., Shen, J., & Yang, D. (2015). Fluorescent Probe HKSOX-1 for Imaging and Detection of Endogenous Superoxide in Live Cells and In Vivo. Journal of the American Chemical Society. https://doi.org/10.1021/jacs.5b01881
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