The DOI matches its Crossref record; 31 of the 31 references it cites were found in a registry or an index.
Shielding against Unfolding by Embedding Enzymes in Metal–Organic Frameworks via a de Novo Approach
Abstract
We show that an enzyme maintains its biological function under a wider range of conditions after being embedded in metal–organic framework (MOF) microcrystals via a de novo approach. This enhanced stability arises from confinement of the enzyme molecules in the mesoporous cavities in the MOFs, which reduces the structural mobility of enzyme molecules. We embedded catalase (CAT) into zeolitic imidazolate frameworks (ZIF-90 and ZIF-8), and then exposed both embedded CAT and free CAT to a denature reagent (i.e., urea) and high temperatures (i.e., 80 °C). The embedded CAT maintains its biological function in the decomposition of hydrogen peroxide even when exposed to 6 M urea and 80 °C, with apparent rate constants k obs (s –1 ) of 1.30 × 10 –3 and 1.05 × 10 –3, respectively, while free CAT shows undetectable activity. A fluorescence spectroscopy study shows that the structural conformation of the embedded CAT changes less under these denaturing conditions than free CAT.
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Cites
Triblock Copolymer Syntheses of Mesoporous Silica with Periodic 50 to 300 Angstrom Pores
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Improvement of enzyme activity, stability and selectivity via immobilization techniques
Mateo, C., Palomo, J. M., Fernandez-Lorente, G., Guisan, J. M., & Fernandez-Lafuente, R. (2007) · Enzyme and Microbial Technology · doi:10.1016/j.enzmictec.2007.01.018 · Cited by 3,375
Industrial biocatalysis today and tomorrow
Schmid, A., Dordick, J. S., Hauer, B., Kiener, A., Wubbolts, M., & Witholt, B. (2001) · Nature · doi:10.1038/35051736 · Cited by 2,405
Rapid synthesis of zeolitic imidazolate framework-8 (ZIF-8) nanocrystals in an aqueous system
Pan, Y., Liu, Y., Zeng, G., Zhao, L., & Lai, Z. (2011) · Chemical Communications · doi:10.1039/c0cc05002d · Cited by 1,735
Biomimetic mineralization of metal-organic frameworks as protective coatings for biomacromolecules
Liang, K., Ricco, R., Doherty, C. M., Styles, M. J., Bell, S., Kirby, N., Mudie, S., Haylock, D., Hill, A. J., Doonan, C. J., et al. (2015) · Nature Communications · doi:10.1038/ncomms8240 · Cited by 1,496
Enzyme-coated carbon nanotubes as single-molecule biosensors
Besteman, K., Lee, J.-O., Wiertz, F. G. M., Heering, H. A., & Dekker, C. (2003) · Nano Letters · doi:10.1021/nl034139u · Cited by 1,294
One-Pot Synthesis of Protein-Embedded Metal–Organic Frameworks with Enhanced Biological Activities
Lyu, F., Zhang, Y., Zare, R. N., Ge, J., & Liu, Z. (2014) · Nano Letters · doi:10.1021/nl5026419 · Cited by 999
Crystals as Molecules: Postsynthesis Covalent Functionalization of Zeolitic Imidazolate Frameworks
Morris, W., Doonan, C. J., Furukawa, H., Banerjee, R., & Yaghi, O. M. (2008) · Journal of the American Chemical Society · doi:10.1021/ja805222x · Cited by 779
Shieh, F.-K., Wang, S.-C., Yen, C.-I., Wu, C.-C., Dutta, S., Chou, L.-Y., Morabito, J. V., Hu, P., Hsu, M.-H., Wu, K. C.-W., et al. (2015) · Journal of the American Chemical Society · doi:10.1021/ja513058h · Cited by 774
Self-citationEnzymatic reactions in confined environments
Küchler, A., Yoshimoto, M., Luginbühl, S., Mavelli, F., & Walde, P. (2016) · Nature Nanotechnology · doi:10.1038/nnano.2016.54 · Cited by 733
Cited by
Multifunctional metal–organic framework heterostructures for enhanced cancer therapy
Liu, J., Huang, J., Zhang, L., & Lei, J. (2021) · Chemical Society Reviews · doi:10.1039/d0cs00178c · Cited by 285
Liao, Y.-T., Nguyen, V. C., Ishiguro, N., Young, A. P., Tsung, C.-K., & Wu, K. C.-W. (2020) · Applied Catalysis B: Environmental · doi:10.1016/j.apcatb.2020.118805 · Cited by 281
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Metadata
- Authors
- Liao, F.-S., Lo, W.-S., Hsu, Y.-S., Wu, C.-C., Wang, S.-C., Shieh, F.-K., Morabito, J. V., Chou, L.-Y., Wu, K. C.-W., & Tsung, C.-K.
- Venue
- Journal of the American Chemical Society
- Publisher
- American Chemical Society (ACS)
- Published
- 2017-05-08
- Volume
- 139
- Issue
- 19
- Pages
- 6530-6533
- ISSN
- 0002-7863 (ISSN-L)
- 1520-5126
- OpenAlex
- W2611605800
- Cited by
- 382
- References
- 31
- FWCI
- 13.78
- Type
- journal article
- Language
- en
- Open access
- closed
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- Liao, F.-S., Lo, W.-S., Hsu, Y.-S., Wu, C.-C., Wang, S.-C., Shieh, F.-K., Morabito, J. V., Chou, L.-Y., Wu, K. C.-W., & Tsung, C.-K. (2017). Shielding against Unfolding by Embedding Enzymes in Metal–Organic Frameworks via a de Novo Approach. Journal of the American Chemical Society. https://doi.org/10.1021/jacs.7b01794
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