Absorption of anthocyanins from blueberries and serum antioxidant status in human subjects
Abstract
In recent years, numerous studies have shown that the polyphenolics present in fruit and vegetable products exhibit a wide range of biological effects. However, there is little reliable information on the absorption of glycosylated and acylated anthocyanins in humans. In the present study, the absorption of anthocyanins in humans was investigated after the consumption of a high-fat meal with a freeze-dried blueberry powder containing 25 individual anthocyanins including 6 acylated structures. Nineteen of the 25 anthocyanins present in the blueberries were detected in human blood serum. Furthermore, the appearance of total anthocyanins in the serum was directly correlated with an increase in serum antioxidant capacity (ORAC acetone, P < 0.01). These results show that anthocyanins can be absorbed in their intact glycosylated and possibly acylated forms in human subjects and that consumption of blueberries, a food source with high in vitro antioxidant properties, is associated with a diet-induced increase in ex vivo serum antioxidant status. Keywords: Anthocyanins; delphinidin; cyanidin; petunidin; peonidin; malvidin; glucoside; galactoside; arabinoside; acetylated; postprandial oxidation; antioxidant activity; functional foods; blueberries; HPLC analysis
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Human intervention studies using chokeberries, cranberries, blueberries, and strawberries (fresh, juice, or freeze-dried), or purified anthocyanin extracts have demonstrated significant improvements in LDL oxidation, lipid peroxidation, total plasma antioxidant capacity, dyslipidemia, and glucose metabolism.
“Human intervention studies using chokeberries, cranberries, blueberries, and strawberries (either fresh, or as juice, or freeze-dried), or purified anthocyanin extracts have demonstrated significant improvements in LDL oxidation, lipid peroxidation, total plasma antioxidant capacity, dyslipidemia, and glucose metabolism.”
From Berries: emerging impact on cardiovascular health (2010) · cites this paper as [39]
Benefits of berry intervention were seen in healthy subjects and in those with existing metabolic risk factors.
“Benefits were seen in healthy subjects and in those with existing metabolic risk factors.”
From Berries: emerging impact on cardiovascular health (2010) · cites this paper as [39]
The most significant outcomes of clinical studies show an increase in plasma or urinary antioxidant capacity, a decrease in LDL oxidation and lipid peroxidation, a decrease in plasma glucose or total cholesterol, and an increase in HDL-cholesterol following berry intervention.
“The most significant outcomes of these clinical studies show an increase in plasma or urinary antioxidant capacity, a decrease in LDL oxidation and lipid peroxidation, a decrease in plasma glucose or total cholesterol, and an increase in HDL-cholesterol following berry intervention.”
From Berries: emerging impact on cardiovascular health (2010) · cites this paper as [39]
Of 20 trials reviewed, nine involved measures of post-prandial status, in which berry consumption was shown to significantly decrease postprandial oxidative stress, especially lipid peroxidation.
“Of 20 trials reviewed, nine involved measures of post-prandial status, in which berry consumption was shown to significantly decrease postprandial oxidative stress, especially lipid peroxidation.”
From Berries: emerging impact on cardiovascular health (2010) · cites this paper as [39]
Clinical studies in healthy humans, subjects with diabetes mellitus, dyslipidemia, metabolic syndrome, hypertension, or in smokers, show a significant decrease in CVD risk factors, especially glucose, lipids and lipid peroxidation, and systolic blood pressure, following berry intervention.
“Clinical studies in healthy humans, subjects with diabetes mellitus, dyslipidemia, metabolic syndrome, hypertension, or in smokers, show a significant decrease in CVD risk factors, especially glucose, lipids and lipid peroxidation, and systolic blood pressure, following berry intervention.”
From Berries: emerging impact on cardiovascular health (2010) · cites this paper as [39]
Cites
Advances in flavonoid research since 1992
Harborne, J. B., & Williams, C. A. (2000) · Phytochemistry · doi:10.1016/s0031-9422(00)00235-1 · Cited by 3,956 · see citing works
Assessing Antioxidant and Prooxidant Activities of Phenolic Compounds
Fukumoto, L., & Mazza, G. A. (2000) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf000220w · Cited by 1,670 · see citing works
Self-citationPrior, R. L., Cao, G., Martín, A., Sofic, E., McEwen, J. J., O'Brien, C., Lischner, N., Ehlenfeldt, M. K., Kalt-Torres, W., Krewer, G. W., et al. (1998) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf980145d · Cited by 1,298 · see citing works
Phenolic compounds and their role in oxidative processes in fruits
Robards, K., Prenzler, P. D., Tucker, G., Swatsitang, P., & Glover, W. (1999) · Food Chemistry · doi:10.1016/s0308-8146(99)00093-x · Cited by 1,277 · see citing works
Antioxidant Capacity, Vitamin C, Phenolics, and Anthocyanins after Fresh Storage of Small Fruits
Kalt, W., Forney, C. F., Martín, A., & Prior, R. L. (1999) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf990266t · Cited by 981 · see citing works
Francis, F. J., & Markakis, P. (1989) · Critical Reviews in Food Science and Nutrition · doi:10.1080/10408398909527503 · Cited by 756 · see citing works
Flavonoids: Dietary occurrence and biochemical activity
Peterson, J. J., & Dwyer, J. T. (1998) · Nutrition Research · doi:10.1016/s0271-5317(98)00169-9 · Cited by 611 · see citing works
Alcohol-Free Red Wine Enhances Plasma Antioxidant Capacity in Humans
Serafini, M., Maiani, G., & Ferro-Luzzi, A. (1998) · Journal of Nutrition · doi:10.1093/jn/128.6.1003 · Cited by 442 · see citing works
Anthocyanins as Antioxidants on Human Low-Density Lipoprotein and Lecithin−Liposome Systems
Satué-Gracia, M. T., Heinonen, M., & Frankel, E. N. (1997) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf970234a · Cited by 368 · see citing works
The identification of flavonoids as glycosides in human plasma
Paganga, G., & Rice-Evans, C. A. (1997) · FEBS Letters · doi:10.1016/s0014-5793(96)01442-1 · Cited by 317 · see citing works
An update on the role of free radicals and antioxidant defense in human disease
Vendemiale, G., Grattagliano, I., & Altomare, E. (1999) · International Journal of Clinical & Laboratory Research · doi:10.1007/s005990050063 · Cited by 271 · see citing works
Bioavailability of Red Wine Anthocyanins As Detected in Human Urine
Lapidot, T., Harel, S., Granit, R., & Kanner, J. (1998) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf980007o · Cited by 161 · see citing works
Kaplan, M., & Aviram, M. (1999) · Clinical Chemistry and Laboratory Medicine (CCLM) · doi:10.1515/cclm.1999.118 · Cited by 157 · see citing works
Antioxidant effect of anthocyanin on enzymatic and non-enzymatic lipid peroxidation
Narayan, M. S., Naidu, K. A., Ravishankar, G. A., et al. (1999) · Prostaglandins Leukotrienes and Essential Fatty Acids · doi:10.1054/plef.1998.0001 · Cited by 105 · see citing works
The new pathophysiology of coronary artery disease
Castelli, W. P. (1998) · The American Journal of Cardiology · doi:10.1016/s0002-9149(98)00729-2 · Cited by 76 · see citing works
Laplaud, P. M., Lelubre, A., & Chapman, M. J. (1997) · Fundamental and Clinical Pharmacology · doi:10.1111/j.1472-8206.1997.tb00166.x · Cited by 53 · see citing works
Postprandial triglyceride metabolism in diabetes mellitus
Georgopoulos, A. (1999) · Clinical Cardiology · doi:10.1002/clc.4960221406 · Cited by 9 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Bioavailability and bioefficacy of polyphenols in humans. I. Review of 97 bioavailability studies
Manach, C., Williamson, G., Morand, C., Scalbert, A., & Rémésy, C. (2005) · The American Journal of Clinical Nutrition · doi:10.1093/ajcn/81.1.230s · Cited by 4,219 · see citing works
Reactive Oxygen Species, Toxicity, Oxidative Stress, and Antioxidants: Chronic Diseases and Aging
Jomova, K., Raptova, R., Alomar, S. Y., Alwasel, S. H., Nepovimova, E., Kuca, K., & Valko, M. (2023) · Archives of Toxicology · doi:10.1007/s00204-023-03562-9 · Cited by 2,638 · see citing works
Berries: emerging impact on cardiovascular health
Basu, A., Rhone, M., & Lyons, T. J. (2010) · Nutrition Reviews · doi:10.1111/j.1753-4887.2010.00273.x · Cited by 462 · see citing works
“…years) High-fat meal (McDonald’s Corp.) High-fat meal supplemented with 100 g freeze-dried wild blueberry powder Increase in serum antioxidant status (P < 0.05) Mazza et al. (2002)39 Postprandial phases, one week apart Single-blind crossover study Five male subjects (mean age, 47 ± 2 years)…”
InfluentialDiet-Derived Phenols in Plasma and Tissues and their Implications for Health
Clifford, M. N. (2004) · Planta Medica · doi:10.1055/s-2004-835835 · Cited by 410 · see citing works
Delphinidin Prevents Muscle Atrophy and Upregulates miR-23a Expression
Murata, M., Nonaka, H., Komatsu, S., Goto, M., Morozumi, M., Yamada, S., Lin, I.-C., Yamashita, S., & Tachibana, H. (2017) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.6b03661 · Cited by 47 · see citing works
- Authors
- Mazza, G., Kay, C. D., Cottrell, T., & Holub, B. J.
- Affiliations
- Agriculture and Agri-Food Canada (CA)
- Corresponding author
- Mazza, G.
- ORCID
- Kay, Colin D.
- Venue
- Journal of Agricultural and Food Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2002-11-16
- Volume
- 50
- Issue
- 26
- Pages
- 7731-7737
- ISSN
- 0021-8561 (ISSN-L)
- 1520-5118
- OpenAlex
- W2147905503
- Cited by
- 484
- References
- 17
- Citations by year
- 2026: 3 · 2025: 8 · 2024: 10 · 2023: 16 · 2022: 11 · 2021: 29 · 2020: 24 · 2019: 17 · 2018: 15 · 2017: 17 · 2016: 16 · 2015: 19 · 2014: 22 · 2013: 37 · 2012: 24
- FWCI
- 12.53
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Agricultural and Food Chemistry (publishedVersion)
- PubMed (publishedVersion)
- UEA Digital Repository (University of East Anglia) (submittedVersion)
- Topics
- Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
- Health Sciences › Nursing › Nutrition and Dietetics › Pomegranate: compositions and health benefits
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Keywords
- anthocyanins, blueberry, serum antioxidant status, acylated anthocyanins, glycosylated anthocyanins, high-fat meal, dietary polyphenols, functional foods
- MeSH terms
- Absorption, Acylation, Anthocyanins, Antioxidants, Chromatography, High Pressure Liquid, Dietary Fats, Fruit, Glucosides, Glycosylation, Humans, Kinetics, Male, Middle Aged, Blueberry Plants
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
