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Screening Methods To Measure Antioxidant Activity of Sorghum (Sorghum bicolor) and Sorghum Products

Awika, Joseph M., Rooney, Lloyd W., Wu, Xianli, Prior, Ronald L., Cisneros-Zevallos, Luis·2003·Journal of Agricultural and Food Chemistry
DOI 10.1021/jf034790i
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Abstract

Specialty sorghums, their brans, and baked and extruded products were analyzed for antioxidant activity using three methods: oxygen radical absorbance capacity (ORAC), 2,2'-azinobis (3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS), and 2,2-diphenyl-1-picrylhydrazyl (DPPH). All sorghum samples were also analyzed for phenolic contents. Both ABTS and DPPH correlated highly with ORAC (R(2) = 0.99 and 0.97, respectively, n = 18). Phenol contents of the sorghums correlated highly with their antioxidant activity measured by the three methods (R(2) >or= 0.96). The ABTS and DPPH methods, which are more cost effective and simpler, were demonstrated to have similar predictive power as ORAC on sorghum antioxidant activity. There is a need to standardize these methods to allow for data comparisons across laboratories.

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Cited by

Metadata

Authors
Awika, J. M., Rooney, L. W., Wu, X., Prior, R. L., & Cisneros-Zevallos, L.
Venue
Journal of Agricultural and Food Chemistry
Publisher
American Chemical Society (ACS)
Published
2003-10-01
Volume
51
Issue
23
Pages
6657-6662
ISSN
  • 0021-8561 (ISSN-L)
  • 1520-5118
DOI
10.1021/jf034790i
OpenAlex
W2112371042
Cited by
802
References
0
FWCI
6.08
Type
journal article
Language
en
Open access
closed

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