The DOI matches its Crossref record; 30 of the 30 references it cites were found in a registry or an index.
Polyelectrolyte Multilayers with a Tunable Young's Modulus: Influence of Film Stiffness on Cell Adhesion
Abstract
Mechanical properties of model and natural gels have recently been demonstrated to play an important role in various cellular processes such as adhesion, proliferation, and differentiation, besides events triggered by chemical ligands. Understanding the biomaterial/cell interface is particularly important in many tissue engineering applications and in implant surgery. One of the final goals would be to control cellular processes precisely at the biomaterial surface and to guide tissue regeneration. In this work, we investigate the substrate mechanical effect on cell adhesion for thin polyelectrolyte multilayer (PEM) films, which can be easily deposited on any type of material. The films were cross linked by means of a water-soluble carbodiimide (EDC), and the film elastic modulus was determined using the AFM nanoindentation technique with a colloidal probe. The Young's modulus could be varied over 2 orders of magnitude (from 3 to 400 kPa) for wet poly(L-lysine)/hyaluronan (PLL/HA) films by changing the EDC concentration. The chemical changes upon cross linking were characterized by means of Fourier transform infrared spectroscopy (FTIR). We demonstrated that the adhesion and spreading of human chondrosarcoma cells directly depend on the Young's modulus. These data indicate that, besides the chemical properties of the polyelectrolytes, the substrate mechanics of PEM films is an important parameter influencing cell adhesion and that PEM offer a new way to prepare thin films of tunable mechanical properties with large potential biomedical applications including drug release.
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Cites
Cell locomotion and focal adhesions are regulated by substrate flexibility
Pelham, R. J., & Wang, Y.-L. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.25.13661 · Cited by 3,027
Effects of substrate stiffness on cell morphology, cytoskeletal structure, and adhesion
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Myotubes differentiate optimally on substrates with tissue-like stiffness
Engler, A. J., Griffin, M. A., Sen, S., Bönnemann, C. G., Sweeney, H. L., & Discher, D. E. (2004) · The Journal of Cell Biology · doi:10.1083/jcb.200405004 · Cited by 1,743
Biomedical surface science: Foundations to frontiers
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Determination of Elastic Moduli of Thin Layers of Soft Material Using the Atomic Force Microscope
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Substrate Compliance versus Ligand Density in Cell on Gel Responses
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Molecular basis for the explanation of the exponential growth of polyelectrolyte multilayers
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Self-citationZero-length crosslinking procedure with the use of active esters
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Neurite branching on deformable substrates
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Buildup Mechanism for Poly(l-lysine)/Hyaluronic Acid Films onto a Solid Surface
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Layer-by-layer Nanoarchitectonics: Invention, Innovation, and Evolution
Ariga, K., Yamauchi, Y., Rydzek, G., Ji, Q., Yonamine, Y., Wu, K. C.-W., & Hill, J. P. (2014) · Chemistry Letters · doi:10.1246/cl.130987 · Cited by 850
Internal structure of polyelectrolyte multilayer assemblies
v. Klitzing, R. (2006) · Physical Chemistry Chemical Physics · doi:10.1039/b607760a · Cited by 431
The elastic modulus of Matrigel™ as determined by atomic force microscopy
Soofi, S. S., Last, J. A., Liliensiek, S. J., Nealey, P. F., & Murphy, C. J. (2009) · Journal of Structural Biology · doi:10.1016/j.jsb.2009.05.005 · Cited by 309
Polyelectrolyte Multilayer Films of Controlled Stiffness Modulate Myoblast Cell Differentiation
Ren, K., Crouzier, T., Roy, C., & Picart, C. (2008) · Advanced Functional Materials · doi:10.1002/adfm.200701297 · Cited by 273
Self-citationFujie, T., Ricotti, L., Desii, A., Menciassi, A., Dario, P., & Mattoli, V. (2011) · Langmuir · doi:10.1021/la203140a · Cited by 52
Metadata
- Authors
- Schneider, A., Francius, G., Obeid, R., Schwinté, P., Hemmerlé, J., Frisch, B., Schaaf, P., Voegel, J.-C., Senger, B., & Picart, C.
- Venue
- Langmuir
- Publisher
- American Chemical Society (ACS)
- Published
- 2005-12-22
- Volume
- 22
- Issue
- 3
- Pages
- 1193-1200
- ISSN
- 0743-7463 (ISSN-L)
- 1520-5827
- OpenAlex
- W2045856259
- Cited by
- 324
- References
- 30
- FWCI
- 7.31
- Type
- journal article
- Language
- en
- Open access
- closed
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- Schneider, A., Francius, G., Obeid, R., Schwinté, P., Hemmerlé, J., Frisch, B., Schaaf, P., Voegel, J.-C., Senger, B., & Picart, C. (2006). Polyelectrolyte Multilayers with a Tunable Young's Modulus: Influence of Film Stiffness on Cell Adhesion. Langmuir. https://doi.org/10.1021/la0521802
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