The DOI matches its Crossref record; 54 of the 54 references it cites were found in a registry or an index.
Polymer-Coated Nanoparticles Interacting with Proteins and Cells: Focusing on the Sign of the Net Charge
Abstract
To study charge-dependent interactions of nanoparticles (NPs) with biological media and NP uptake by cells, colloidal gold nanoparticles were modified with amphiphilic polymers to obtain NPs with identical physical properties except for the sign of the charge (negative/positive). This strategy enabled us to solely assess the influence of charge on the interactions of the NPs with proteins and cells, without interference by other effects such as different size and colloidal stability. Our study shows that the number of adsorbed human serum albumin molecules per NP was not influenced by their surface charge. Positively charged NPs were incorporated by cells to a larger extent than negatively charged ones, both in serum-free and serum-containing media. Consequently, with and without protein corona (i.e., in serum-free medium) present, NP internalization depends on the sign of charge. The uptake rate of NPs by cells was higher for positively than for negatively charged NPs. Furthermore, cytotoxicity assays revealed a higher cytotoxicity for positively charged NPs, associated with their enhanced uptake.
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Understanding biophysicochemical interactions at the nano–bio interface
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Cellular uptake of nanoparticles: Journey inside the cell
Behzadi, S., Serpooshan, V., Tao, W., Hamaly, M. A., Alkawareek, M. Y., Dreaden, E. C., Brown, D., Alkilany, A. M., Farokhzad, O. C., & Mahmoudi, M. (2017) · Chemical Society Reviews · doi:10.1039/c6cs00636a · Cited by 2,859
Rapid formation of plasma protein corona critically affects nanoparticle pathophysiology
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Self-citationNanoparticle colloidal stability in cell culture media and impact on cellular interactions
Moore, T. L., Rodriguez-Lorenzo, L., Hirsch, V., Balog, S., Urban, D., Jud, C., Rothen-Rutishauser, B., Lattuada, M., & Petri-Fink, A. (2015) · Chemical Society Reviews · doi:10.1039/c4cs00487f · Cited by 1,082
Gold Nanoparticles in Diagnostics and Therapeutics for Human Cancer
Singh, P., Pandit, S., Mokkapati, V. R. S. S., Garg, A., Ravikumar, V., & Mijakovic, I. (2018) · International Journal of Molecular Sciences · doi:10.3390/ijms19071979 · Cited by 1,038
Metadata
- Authors
- Hühn, D., Kantner, K., Geidel, C., Brandholt, S., De Cock, I., Soenen, S. J. H., Rivera_Gil, P., Montenegro, J.-M., Braeckmans, K., Müllen, K., Nienhaus, G. U., Klapper, M., & Parak, W. J.
- Venue
- ACS Nano
- Publisher
- American Chemical Society (ACS)
- Published
- 2013-04-08
- Volume
- 7
- Issue
- 4
- Pages
- 3253-3263
- ISSN
- 1936-0851 (ISSN-L)
- 1936-086X
- OpenAlex
- W2045847395
- Cited by
- 580
- References
- 54
- FWCI
- 20.73
- Type
- journal article
- Language
- en
- Open access
- closed
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- Hühn, D., Kantner, K., Geidel, C., Brandholt, S., De Cock, I., Soenen, S. J. H., Rivera_Gil, P., Montenegro, J.-M., Braeckmans, K., Müllen, K., Nienhaus, G. U., Klapper, M., & Parak, W. J. (2013). Polymer-Coated Nanoparticles Interacting with Proteins and Cells: Focusing on the Sign of the Net Charge. ACS Nano. https://doi.org/10.1021/nn3059295
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