Effects of Probiotic Lactobacillus Paracasei Treatment on the Host Gut Tissue Metabolic Profiles Probed via Magic-Angle-Spinning NMR Spectroscopy
Abstract
We have used a simplified gnotobiotic mouse model to evaluate the effects of single bacterial species, Lactobacillus paracasei NCC2461, on the metabolic profiles of intact intestinal tissues using high-resolution magic-angle-spinning 1H NMR spectroscopy (HRMAS). A total of 24 female gnotobiotic mice were divided into three groups: a control group supplemented with water and two groups supplemented with either live L. paracasei or a gamma-irradiated equivalent. HRMAS was used to characterize the biochemical components of intact epithelial tissues from the duodenum, jejunum, ileum, proximal, and distal colons in all animals and data were analyzed using chemometrics. Variations in relative concentrations of amino acids, anti-oxidant, and creatine were observed relating to different physiological properties in each intestinal tissue. Metabolic characteristics of lipogenesis and fat storage were observed in the jejunum and colon. Colonization with live L. paracasei induced region-dependent changes in the metabolic profiles of all intestinal tissues, except for the colon, consistent with modulation of intestinal digestion, absorption of nutrients, energy metabolism, lipid synthesis and protective functions. Ingestion of gamma-irradiated bacteria produced no effects on the observed metabolic profiles. 1H MAS NMR spectroscopy was able to generate characteristic metabolic signatures reflecting the structure and function of intestinal tissues. These signals acted as reference profiles with which to compare changes in response to gut microbiota manipulation at the tissue level as demonstrated by ingestion of a bacterial probiotic.
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In mice, L. rhamnosus supplementation elevated plasma choline levels compared to L. paracasei and controls.
“Elevated choline levels were observed in plasma of mice supplemented with L. rhamnosus compared to those fed with L. paracasei and controls [283].”
From International Society of Sports Nutrition Position Stand: Probiotics (2019) · cites this paper as [283]
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- Authors
- Martin, F.-P. J., Wang, Y., Sprenger, N., Holmes, E., Lindon, J. C., Kochhar, S., & Nicholson, J. K.
- Affiliations
- Department of Biomolecular Medicine, Division of Surgery, Oncology, Reproductive Biology and Anaesthetics, Faculty of Medicine, Imperial College London, Sir Alexander Fleming Building, South Kensington, London SW7 2AZ, UK, and Department of Nutrition and Health and Bioanalytical Sciences, Nestlé Research Center, P. O. Box 44, Vers-chez-les-Blanc, CH-1000 Lausanne 26, Switzerland
- Nestlé (United Kingdom) (GB)
- Corresponding author
- Nicholson, Jeremy K.
- Venue
- Journal of Proteome Research
- Publisher
- American Chemical Society (ACS)
- Published
- 2007-02-23
- Volume
- 6
- Issue
- 4
- Pages
- 1471-1481
- ISSN
- 1535-3893 (ISSN-L)
- 1535-3907
- OpenAlex
- W2127385968
- Cited by
- 87
- References
- 41
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 1 · 2022: 4 · 2021: 1 · 2020: 2 · 2019: 4 · 2018: 3 · 2016: 2 · 2015: 2 · 2014: 9 · 2013: 6 · 2012: 10
- FWCI
- 18.41
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Proteome Research (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
- Keywords
- Lactobacillus paracasei, probiotics, gnotobiotic mice, gut microbiota, amino acids, antioxidants, creatine, lipogenesis, fat deposition, energy metabolism
- MeSH terms
- Amino Acids, Animals, Energy Metabolism, Female, Germ-Free Life, Inflammation, Intestinal Mucosa, Intestines, Lactobacillus, Lipids, Mice, Inbred Strains, Muscle Contraction, Nuclear Magnetic Resonance, Biomolecular, Probiotics, Mice
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