Influence of microbial species on small intestinal myoelectric activity and transit in germ-free rats
Abstract
The effect of an intestinal microflora consisting of selected microbial species on myoelectric activity of small intestine was studied using germ-free rat models, with recording before and after specific intestinal colonization, in the unanesthetized state. Intestinal transit, neuropeptides in blood (RIA), and neuromessengers in the intestinal wall were determined. Clostridium tabificum vp 04 promoted regular spike burst activity, shown by a reduction of the migrating myoelectric complex (MMC) period from 30.5 ± 3.9 min in the germ-free state to 21.2 ± 0.14 min ( P < 0.01). Lactobacillus acidophilus A10 and Bifidobacterium bifidum B11 reduced the MMC period from 27.9 ± 4.5 to 21.5 ± 2.1 min ( P < 0.02) and accelerated small intestinal transit ( P < 0.05). Micrococcus luteus showed an inhibitory effect, with an MMC period of 35.9 ± 9.3 min compared with 27.7 ± 6.3 min in germ-free rats ( P < 0.01). Inhibition was indicated also for Escherichia coli X7gnotobiotic rats. No consistent changes in slow wave frequency were observed. The concentration of neuropeptide Y in blood decreased after introduction of conventional intestinal microflora, suggesting reduced inhibitory control. Intestinal bacteria promote or suppress the initiation and aboral migration of the MMC depending on the species involved. Bacteria with primitive fermenting metabolism (anaerobes) emerge as important promoters of regular spike burst activity in small intestine.
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Gut microbes can accelerate GI transit.
“Gut microbes can accelerate GI transit51, 52.”
From The gut Microbiome in adult and pediatric functional gastrointestinal disorders (2019) · cites this paper as [51]
Plasma NPY levels decreased after the introduction of conventional intestinal microbiota in germ-free mice.
“In contrast, plasma NPY levels decreased after the introduction of conventional intestinal microbiota in germ-free mice [217], a shift also observed by Sudo et al. [216] with the corticosterone levels after recolonization of germ-free mice.”
From Anxiety, depression, and the microbiome: A role for gut peptides (2018) · cites this paper as [217]
The enteric microbiota can directly influence gut homeostasis by the regulation of bowel motility and modulation of intestinal pain, immune responses, and nutrient processing.
“The enteric microbiota can directly influence gut homeostasis by the regulation of bowel motility and modulation of intestinal pain, immune responses, and nutrient processing.8-10”
From Principles and clinical implications of the brain-gut-enteric microbiota axis (2009) · cites this paper as [8]
Gut microbiota modulates gut motor function, which in turn can alter intestinal microbiota composition.
“Nonetheless gut microbiota modulates gut motor function, which in turn can alter the intestinal microbiota composition.”
From Gut microbiota role in irritable bowel syndrome: New therapeutic strategies (2016) · cites this paper as [96]
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“, 2005), and another group demonstrated that intestinal bacteria could influence the migrating myoelectric complex, which are waves of electrical activity that sweep through the intestine in a regular cycle during fasting in a germ-free rat model (Husebye et al., 2001).”
Background
- Authors
- Husebye, E., Hellström, P. M., Sundler, F., Chen, J., & Midtvedt, T.
- Affiliations
- Oslo University Hospital (NO)
- Karolinska University Hospital (SE)
- Lund University (SE)
- Karolinska Institutet (SE)
- Venue
- American Journal of Physiology-Gastrointestinal and Liver Physiology
- Publisher
- American Physiological Society
- Published
- 2001-03-01
- Volume
- 280
- Issue
- 3
- Pages
- G368-G380
- ISSN
- 0193-1857 (ISSN-L)
- 1522-1547
- OpenAlex
- W2169050068
- Cited by
- 319
- References
- 48
- Citations by year
- 2026: 10 · 2025: 12 · 2024: 12 · 2023: 13 · 2022: 18 · 2021: 17 · 2020: 12 · 2019: 20 · 2018: 16 · 2017: 16 · 2016: 16 · 2015: 22 · 2014: 13 · 2013: 15 · 2012: 13
- FWCI
- 4.39
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- American Journal of Physiology-Gastrointestinal and Liver Physiology (publishedVersion)
- PubMed (publishedVersion)
- Lund University Publications (Lund University) (submittedVersion)
- Topics
- Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Keywords
- gut microbiota, migrating motor complex, small intestine motility, germ-free rats, Lactobacillus acidophilus, Bifidobacterium bifidum, Micrococcus luteus, Escherichia coli, neuropeptide Y, slow wave frequency, gnotobiotic rats, anaerobic bacteria
- MeSH terms
- Action Potentials, Animals, Bacteria, Bifidobacterium, Clostridium, Escherichia coli, Gastrointestinal Motility, Germ-Free Life, Intestine, Small, Lactobacillus acidophilus, Male, Neuropeptide Y, Species Specificity, Myoelectric Complex, Migrating, Micrococcus luteus, Rats, Sprague-Dawley, Rats
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