Cited by 319Gastrointestinal motility and disordersGut microbiota and healthProbiotics and Fermented Foods

Influence of microbial species on small intestinal myoelectric activity and transit in germ-free rats

Husebye, Einar, Hellström, Per M., Sundler, Frank, Chen, Jie, Midtvedt, Tore·2001·American Journal of Physiology-Gastrointestinal and Liver Physiology

Abstract

The effect of an intestinal microflora consisting of selected microbial species on myoelectric activity of small intestine was studied using germ-free rat models, with recording before and after specific intestinal colonization, in the unanesthetized state. Intestinal transit, neuropeptides in blood (RIA), and neuromessengers in the intestinal wall were determined. Clostridium tabificum vp 04 promoted regular spike burst activity, shown by a reduction of the migrating myoelectric complex (MMC) period from 30.5 ± 3.9 min in the germ-free state to 21.2 ± 0.14 min ( P < 0.01). Lactobacillus acidophilus A10 and Bifidobacterium bifidum B11 reduced the MMC period from 27.9 ± 4.5 to 21.5 ± 2.1 min ( P < 0.02) and accelerated small intestinal transit ( P < 0.05). Micrococcus luteus showed an inhibitory effect, with an MMC period of 35.9 ± 9.3 min compared with 27.7 ± 6.3 min in germ-free rats ( P < 0.01). Inhibition was indicated also for Escherichia coli X7gnotobiotic rats. No consistent changes in slow wave frequency were observed. The concentration of neuropeptide Y in blood decreased after introduction of conventional intestinal microflora, suggesting reduced inhibitory control. Intestinal bacteria promote or suppress the initiation and aboral migration of the MMC depending on the species involved. Bacteria with primitive fermenting metabolism (anaerobes) emerge as important promoters of regular spike burst activity in small intestine.

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Registry

Crossref · 10.1152/ajpgi.2001.280.3.g368

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48 of 48 references verified0 citation errors · 0 not checkable

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  • Gut microbes can accelerate GI transit.

    “Gut microbes can accelerate GI transit51, 52.”
  • Plasma NPY levels decreased after the introduction of conventional intestinal microbiota in germ-free mice.

    “In contrast, plasma NPY levels decreased after the introduction of conventional intestinal microbiota in germ-free mice [217], a shift also observed by Sudo et al. [216] with the corticosterone levels after recolonization of germ-free mice.”
  • The enteric microbiota can directly influence gut homeostasis by the regulation of bowel motility and modulation of intestinal pain, immune responses, and nutrient processing.

    “The enteric microbiota can directly influence gut homeostasis by the regulation of bowel motility and modulation of intestinal pain, immune responses, and nutrient processing.8-10”
  • Gut microbiota modulates gut motor function, which in turn can alter intestinal microbiota composition.

    “Nonetheless gut microbiota modulates gut motor function, which in turn can alter the intestinal microbiota composition.”

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Authors
Husebye, E., Hellström, P. M., Sundler, F., Chen, J., & Midtvedt, T.
Affiliations
  • Oslo University Hospital (NO)
  • Karolinska University Hospital (SE)
  • Lund University (SE)
  • Karolinska Institutet (SE)
Venue
American Journal of Physiology-Gastrointestinal and Liver Physiology
Published
2001-03-01
Volume
280
Issue
3
Pages
G368-G380
ISSN
  • 0193-1857 (ISSN-L)
  • 1522-1547
OpenAlex
W2169050068
Cited by
319
References
48
Citations by year
2026: 10 · 2025: 12 · 2024: 12 · 2023: 13 · 2022: 18 · 2021: 17 · 2020: 12 · 2019: 20 · 2018: 16 · 2017: 16 · 2016: 16 · 2015: 22 · 2014: 13 · 2013: 15 · 2012: 13
FWCI
4.39
Type
journal article
Language
en
Open access
closed
Locations
  • American Journal of Physiology-Gastrointestinal and Liver Physiology (publishedVersion)
  • PubMed (publishedVersion)
  • Lund University Publications (Lund University) (submittedVersion)
Topics
  • Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
Keywords
gut microbiota, migrating motor complex, small intestine motility, germ-free rats, Lactobacillus acidophilus, Bifidobacterium bifidum, Micrococcus luteus, Escherichia coli, neuropeptide Y, slow wave frequency, gnotobiotic rats, anaerobic bacteria
MeSH terms
Action Potentials, Animals, Bacteria, Bifidobacterium, Clostridium, Escherichia coli, Gastrointestinal Motility, Germ-Free Life, Intestine, Small, Lactobacillus acidophilus, Male, Neuropeptide Y, Species Specificity, Myoelectric Complex, Migrating, Micrococcus luteus, Rats, Sprague-Dawley, Rats

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