Differential Proteomic Analysis Distinguishes Tissue Repair Biomarker Signatures in Wound Exudates Obtained from Normal Healing and Chronic Wounds
Abstract
Chronic wounds associated with vascular disease, diabetes mellitus, or aging are leading causes of morbidity in western countries and represent an unresolved clinical problem. The development of innovative strategies to promote tissue repair is therefore an important task that requires a more thorough analysis of the underlying molecular pathophysiology. We propose that the understanding of the complex biological events that control tissue repair or its failure largely benefits from a broad analytical approach as provided by novel proteomic methodologies. Here we present the first comparative proteome analysis of wound exudates obtained from normal healing or nonhealing (venous leg ulcer) human skin wounds. A total of 149 proteins were identified with high confidence. A minority of proteins was exclusively present in exudate of the healing wound (23 proteins) or the nonhealing wound (26 proteins). Of particular interest was the differential distribution of specific proteins among the two different healing phenotypes. Whereas in the exudate obtained from the healing wound mediators characteristic for tissue formation were abundantly present, in the exudate obtained from the nonhealing wound numerous mediators characteristic for a persistent inflammatory and tissue destructive response were identified. Furthermore, the study also revealed interesting results regarding the identification of new proteins with yet unknown functions in skin repair. This analysis therefore represents an important basis for the search for potential biomarkers, which give rise to a better understanding and monitoring of disease progression in chronic wounds.
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Matrix metalloproteinases such as collagenase and gelatinases A and B are elevated in chronic wound fluids compared to acute wound fluid.
“It has been shown that matrix metalloproteinases (MMPs), such as collagenase and gelatinases A and B, are elevated in chronic wound fluids when compared to acute wound fluid (25, 38).”
From Wound repair and regeneration: Mechanisms, signaling, and translation (2014) · cites this paper as [25]
Recent investigations of chronic wound tissue and fluid indicate a continual competition between inflammatory and anti-inflammatory signals leading to a misbalanced environment for proper wound healing.
“Specifically, recent investigations of chronic wound tissue and fluid indicate a continual competition between inflammatory and anti-inflammatory signals leading to a misbalanced environment for proper wound healing to occur (25, 26).”
From Wound repair and regeneration: Mechanisms, signaling, and translation (2014) · cites this paper as [25]
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- Authors
- Eming, S. A., Koch, M., Krieger, A., Brachvogel, B., Kreft, S., Bruckner-Tuderman, L., Krieg, T., Shannon, J. D., & Fox, J. W.
- Affiliations
- University of Freiburg (DE)
- Department of Dermatology, Center for Molecular Medicine Cologne (CMMC), Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), Institute for Oral and Musculoskeletal Biology, and Center for Biochemistry, University of Cologne, Cologne, Germany, Department of Dermatology, University Medical Center Freiburg, Germany, Freiburg Institute of Advanced Studies, School of Life Sciences-LifeNet, Germany, and Department of Microbiology, University of Virginia,...
- Corresponding author
- Eming, Sabine A.
- Venue
- Journal of Proteome Research
- Publisher
- American Chemical Society (ACS)
- Published
- 2010-08-11
- Volume
- 9
- Issue
- 9
- Pages
- 4758-4766
- ISSN
- 1535-3893 (ISSN-L)
- 1535-3907
- OpenAlex
- W2042841861
- Cited by
- 248
- References
- 48
- Citations by year
- 2026: 6 · 2025: 14 · 2024: 13 · 2023: 14 · 2022: 19 · 2021: 23 · 2020: 17 · 2019: 18 · 2018: 24 · 2017: 17 · 2016: 14 · 2015: 13 · 2014: 17 · 2013: 17 · 2012: 11
- FWCI
- 12.63
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Proteome Research (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Rehabilitation › Wound Healing and Treatments
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Protease and Inhibitor Mechanisms
- Keywords
- chronic wounds, wound exudate, venous leg ulcers, tissue repair, wound healing biomarkers, differentially expressed proteins, chronic inflammation
- MeSH terms
- Aged, Chronic Disease, Electrophoresis, Polyacrylamide Gel, Exudates and Transudates, Humans, Immunohistochemistry, Inflammation, Lactoferrin, Leg Ulcer, Middle Aged, Wound Healing, Reproducibility of Results, Biomarkers, Extracellular Matrix Proteins, Annexins, Proteome, Calgranulin B, Proteomics
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