Cited by 376Metabolomics and Mass Spectrometry StudiesDiet, Metabolism, and DiseaseAdipokines, Inflammation, and Metabolic Diseases

Metabolomic Analysis of Livers and Serum from High-Fat Diet Induced Obese Mice

Kim, Hyun-Jin, Kim, Jin Hee, Noh, Siwon, Hur, Haeng Jeon, Sung, Mi Jeong, Hwang, Jin-Taek, Park, Jae Ho, Yang, Hye Jeong +3 more·2011·Journal of Proteome Research

Abstract

Liver and serum metabolites of obese and lean mice fed on high fat or normal diets were analyzed using ultraperformance liquid chromatography-quadrupole-time-of-flight mass spectrometry, gas chromatography-mass spectrometry, and partial least-squares-discriminant analysis (PLS-DA). Obese and lean groups were clearly discriminated from each other on PLS-DA score plot and major metabolites contributing to the discrimination were assigned as lipid metabolites (fatty acids, phosphatidylcholines (PCs), and lysophosphatidylcholines (lysoPCs)), lipid metabolism intermediates (betaine, carnitine, and acylcarnitines), amino acids, acidic compounds, monosaccharides, and serotonin. A high-fat diet increased lipid metabolites but decreased lipid metabolism intermediates and the NAD/NADH ratio, indicating that abnormal lipid and energy metabolism induced by a high-fat diet resulted in fat accumulation via decreased β-oxidation. In addition, this study revealed that the levels of many metabolites, including serotonin, betaine, pipecolic acid, and uric acid, were positively or negatively related to obesity-associated diseases. On the basis of these metabolites, we proposed a metabolic pathway related to high-fat diet-induced obesity. These metabolites can be used to better understand obesity and related diseases induced by a hyperlipidic diet. Furthermore, the level changes of these metabolites can be used to assess the risk of obesity and the therapeutic effect of obesity management.

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Crossref · 10.1021/pr100892r

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Authors
Kim, H.-J., Kim, J. H., Noh, S., Hur, H. J., Sung, M. J., Hwang, J.-T., Park, J. H., Yang, H. J., Kim, M.-S., Kwon, D. Y., & Yoon, S. H.
Affiliations
Korea Food Research Institute (KR)
Corresponding author
  • Kwon, Dae Young
  • Yoon, Suk Hoo
Venue
Journal of Proteome Research
Published
2010-11-24
Volume
10
Issue
2
Pages
722-731
ISSN
  • 1535-3893 (ISSN-L)
  • 1535-3907
OpenAlex
W2114627400
Cited by
376
References
42
Citations by year
2025: 16 · 2024: 18 · 2023: 20 · 2022: 26 · 2021: 26 · 2020: 42 · 2019: 22 · 2018: 27 · 2017: 34 · 2016: 28 · 2015: 27 · 2014: 26 · 2013: 36 · 2012: 18
FWCI
8.20
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Proteome Research (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diet, Metabolism, and Disease
  • Health Sciences › Medicine › Epidemiology › Adipokines, Inflammation, and Metabolic Diseases
Keywords
high-fat diet-induced obesity, liver metabolomics, serum metabolomics, lipid metabolism, fatty acids, phosphatidylcholine, lysophosphatidylcholine, acylcarnitines, betaine, L-carnitine, NAD+/NADH ratio, fatty acid beta-oxidation, fat deposition, serotonin, pipecolic acid, uric acid, obesity-related comorbidities, metabolic pathways
MeSH terms
Animals, Chromatography, High Pressure Liquid, Dietary Fats, Histocytochemistry, Humans, Liver, Lysophosphatidylcholines, Male, Gas Chromatography-Mass Spectrometry, Mice, Inbred C57BL, Mice, Obese, Obesity, Multivariate Analysis, Discriminant Analysis, Least-Squares Analysis, Lipid Metabolism, Mice, Metabolic Networks and Pathways, Metabolomics, Hep G2 Cells

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