Metabolomic Analysis of Livers and Serum from High-Fat Diet Induced Obese Mice
Abstract
Liver and serum metabolites of obese and lean mice fed on high fat or normal diets were analyzed using ultraperformance liquid chromatography-quadrupole-time-of-flight mass spectrometry, gas chromatography-mass spectrometry, and partial least-squares-discriminant analysis (PLS-DA). Obese and lean groups were clearly discriminated from each other on PLS-DA score plot and major metabolites contributing to the discrimination were assigned as lipid metabolites (fatty acids, phosphatidylcholines (PCs), and lysophosphatidylcholines (lysoPCs)), lipid metabolism intermediates (betaine, carnitine, and acylcarnitines), amino acids, acidic compounds, monosaccharides, and serotonin. A high-fat diet increased lipid metabolites but decreased lipid metabolism intermediates and the NAD/NADH ratio, indicating that abnormal lipid and energy metabolism induced by a high-fat diet resulted in fat accumulation via decreased β-oxidation. In addition, this study revealed that the levels of many metabolites, including serotonin, betaine, pipecolic acid, and uric acid, were positively or negatively related to obesity-associated diseases. On the basis of these metabolites, we proposed a metabolic pathway related to high-fat diet-induced obesity. These metabolites can be used to better understand obesity and related diseases induced by a hyperlipidic diet. Furthermore, the level changes of these metabolites can be used to assess the risk of obesity and the therapeutic effect of obesity management.
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HFD reduces NAD+ content in diverse tissues.
“HFD also reduces the NAD+ content in diverse tissues, which is likely to impair SIRT1 activation (Kim et al, 2011; Yoshino et al, 2011) (Fig 3B).”
From Mitochondrial response to nutrient availability and its role in metabolic disease (2014) · cites this paper as Kim et al, 2011
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- Authors
- Kim, H.-J., Kim, J. H., Noh, S., Hur, H. J., Sung, M. J., Hwang, J.-T., Park, J. H., Yang, H. J., Kim, M.-S., Kwon, D. Y., & Yoon, S. H.
- Affiliations
- Korea Food Research Institute (KR)
- Corresponding author
- Kwon, Dae Young
- Yoon, Suk Hoo
- ORCID
- Venue
- Journal of Proteome Research
- Publisher
- American Chemical Society (ACS)
- Published
- 2010-11-24
- Volume
- 10
- Issue
- 2
- Pages
- 722-731
- ISSN
- 1535-3893 (ISSN-L)
- 1535-3907
- OpenAlex
- W2114627400
- Cited by
- 376
- References
- 42
- Citations by year
- 2025: 16 · 2024: 18 · 2023: 20 · 2022: 26 · 2021: 26 · 2020: 42 · 2019: 22 · 2018: 27 · 2017: 34 · 2016: 28 · 2015: 27 · 2014: 26 · 2013: 36 · 2012: 18
- FWCI
- 8.20
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Proteome Research (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diet, Metabolism, and Disease
- Health Sciences › Medicine › Epidemiology › Adipokines, Inflammation, and Metabolic Diseases
- Keywords
- high-fat diet-induced obesity, liver metabolomics, serum metabolomics, lipid metabolism, fatty acids, phosphatidylcholine, lysophosphatidylcholine, acylcarnitines, betaine, L-carnitine, NAD+/NADH ratio, fatty acid beta-oxidation, fat deposition, serotonin, pipecolic acid, uric acid, obesity-related comorbidities, metabolic pathways
- MeSH terms
- Animals, Chromatography, High Pressure Liquid, Dietary Fats, Histocytochemistry, Humans, Liver, Lysophosphatidylcholines, Male, Gas Chromatography-Mass Spectrometry, Mice, Inbred C57BL, Mice, Obese, Obesity, Multivariate Analysis, Discriminant Analysis, Least-Squares Analysis, Lipid Metabolism, Mice, Metabolic Networks and Pathways, Metabolomics, Hep G2 Cells
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