The DOI matches its Crossref record; 53 of the 53 references it cites were found in a registry or an index.
In Vitro and in Vivo Interactions of Bisphenol A and Its Metabolite, Bisphenol A Glucuronide, with Estrogen Receptors α and β
Abstract
The estrogenic activities of bisphenol A (BPA) and its major metabolite BPA glucuronide (BPA-G) were assessed in a number of in vitro and in vivo assays. BPA competed with [3H]-17beta-estradiol (E2) for binding to mouse uterine cytosol ER, a glutathione S-transferase (GST)-human ER D, E, and F domain fusion protein (GST-hERalphadef) and full-length recombinant hERbeta. The IC(50) values for E2 were similar for all three receptor preparations, whereas BPA competed more effectively for binding to hERbeta (0.96 microM) than to either mouse uterine cytosol ER (26 microM) or GST-hERalphadef (36 microM). In contrast, BPA-G did not competitively displace [3H]E2 from any of the ER preparations. In MCF-7 cells transiently transfected with Gal4-hERalphadef or Gal4-hERbetadef, BPA induced reporter gene activity with comparable EC(50) values (71 and 39 microM, respectively). No significant induction of reporter gene activity was seen for BPA-G. Cotreatment studies showed that concentrations of (10 microM) BPA and BPA-G did not antagonize E2-induced luciferase mediated through either Gal4-hERalphadef or Gal4-hERbetadef. In vivo, the uterotropic effect of gavage or subcutaneous (sc) administration of 0.002-800 mg of BPA/kg of body weight/day for three consecutive days was examined in immature rats. Dose-related estrogenic effects on the rat uterus were observed at oral doses of 200 and 800 mg/kg and at sc doses of 10, 100, and 800 mg/kg. These results demonstrate that BPA competes more effectively for binding to ERbeta, but induces ERalpha- and ERbeta-mediated gene expression with comparable efficacy. In contrast, BPA-G did not exhibit any in vitro estrogenic activity. In addition, there was a clear route dependency on the ability of BPA to induce estrogenic responses in vivo.
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Cites
Molecular Cloning: A Laboratory Manual
Sambrook, J., Fritsch, E., & Maniatis, T. (1989) · UCLA - Biblioteca de Ciencias de la Salud · Cited by 133,589
Molecular cloning: A laboratory manual
Deininger, P. (1990) · Analytical Biochemistry · doi:10.1016/0003-2697(90)90595-z · Cited by 84,682
The Steroid and Thyroid Hormone Receptor Superfamily
Evans, R. M. (1988) · Science · doi:10.1126/science.3283939 · Cited by 7,439
Cloning of a novel receptor expressed in rat prostate and ovary.
Kuiper, G. G., Enmark, E., Pelto-Huikko, M., Nilsson, S., & Gustafsson, J. A. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.12.5925 · Cited by 4,788
Kuiper, G. G. J. M., Carlsson, B., Grandien, K., Enmark, E., Häggblad, J., Nilsson, S., & Gustafsson, J.-A. (1997) · Endocrinology · doi:10.1210/endo.138.3.4979 · Cited by 4,442
Interaction of Estrogenic Chemicals and Phytoestrogens with Estrogen Receptor β
Kuiper, G. G. J. M., Lemmen, J. G., Carlsson, B., Corton, J. C., Safe, S. H., van der Saag, P. T., van der Burg, B., & Gustafsson, J.-Å. (1998) · Endocrinology · doi:10.1210/endo.139.10.6216 · Cited by 4,203
Molecular basis of agonism and antagonism in the oestrogen receptor
Brzozowski, A. M., Pike, A. C. W., Dauter, Z., Hubbard, R. E., Bonn, T., Engström, O., Öhman, L., Greene, G. L., Gustafsson, J.-Å., & Carlquist, M. (1997) · Nature · doi:10.1038/39645 · Cited by 3,355
ERβ: Identification and characterization of a novel human estrogen receptor
Mosselman, S., Polman, J., & Dijkema, R. (1996) · FEBS Letters · doi:10.1016/0014-5793(96)00782-x · Cited by 2,269
Are oestrogens involved in falling sperm counts and disorders of the male reproductive tract?
Sharpe, R. M., & Skakkebaek, N. E. (1993) · The Lancet · doi:10.1016/0140-6736(93)90953-e · Cited by 1,849
Bisphenol-A: an estrogenic substance is released from polycarbonate flasks during autoclaving
Krishnan, A. V., Stathis, P., Permuth, S. F., Tokes, L., & Feldman, D. I. (1993) · Endocrinology · doi:10.1210/en.132.6.2279 · Cited by 1,613
Cited by
In vitro molecular mechanisms of bisphenol A action.
Wetherill, Y. B., Akingbemi, B. T., Kanno, J., McLachlan, J. A., Nadal, A., Sonnenschein, C., Watson, C. S., Zoeller, R. T., & Belcher, S. M. (2007) · Reproductive Toxicology · doi:10.1016/j.reprotox.2007.05.010 · Cited by 920
Parent bisphenol A accumulation in the human maternal-fetal-placental unit.
Schönfelder, G., Wittfoht, W., Hopp, H., Talsness, C. E., Paul, M., & Chahoud, I. (2002) · Environmental Health Perspectives · doi:10.1289/ehp.021100703 · Cited by 668
Moreman, J., Lee, O., Trznadel, M., David, A., Kudoh, T., & Tyler, C. R. (2017) · Environmental Science & Technology · doi:10.1021/acs.est.7b03283 · Cited by 527
Kinch, C. D., Ibhazehiebo, K., Jeong, J.-H., Habibi, H. R., & Kurrasch, D. M. (2015) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1417731112 · Cited by 485
Transfer of bisphenol A across the human placenta
Balakrishnan, B., Henare, K., Thorstensen, E. B., Ponnampalam, A. P., & Mitchell, M. D. (2010) · American Journal of Obstetrics and Gynecology · doi:10.1016/j.ajog.2010.01.025 · Cited by 304
Metadata
- Authors
- Matthews, J. B., Twomey, K., & Zacharewski, T. R.
- Venue
- Chemical Research in Toxicology
- Publisher
- American Chemical Society (ACS)
- Published
- 2000-12-30
- Volume
- 14
- Issue
- 2
- Pages
- 149-157
- ISSN
- 0893-228X (ISSN-L)
- 1520-5010
- OpenAlex
- W1979440858
- Cited by
- 465
- References
- 53
- FWCI
- 9.33
- Type
- journal article
- Language
- en
- Open access
- closed
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