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Cited by 183Origins and Evolution of LifeRNA and protein synthesis mechanismsDNA and Nucleic Acid Chemistry

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Montmorillonite Catalysis of 30–50 Mer Oligonucleotides: Laboratory Demonstration of Potential Steps in the Origin of the RNA World

Ferris, James P.·2002·Origins of life and evolution of the biosphere
DOI 10.1023/a:1020543312109
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Abstract

Elongation of the primer 32pdA(pdA)8pA proceeds by the reaction of the 5'-phosphorimidazolides of adenosine and uridine in the presence of montmorillonite clay. Daily addition of the activated nucleotides for up to 14 days results in the formation of 40-50 mers using the 5'-phosphorimidazolide of adenosine (ImpA) and 25-30 mers using the 5'-phosphorimidazolide of uridine (ImpU). The limitation on the lengths of the chains formed is not due to the inhibitors formed since the same chain lengths were formed using 2-3 times the amount of montmorillonite catalyst. The shorter oligomers formed by the addition of U monomers is not due to its greater rate of decomposition since it was found that both the A and the U adducts decompose at about the same rates. Alkaline phosphatase hydrolysis studies revealed that some of the oligomers are capped at the 5'-end to form, with ImpA, Ap32pdA(pdA)8pA(pA)n. The extent of capping depends on the reaction time and the purine or pyrimidine base in the activated mononucleotide. Hydrolysis with ribonuclease T2 followed by alkaline phosphatase determined the sites of the 3', 5'- and 2', 5'-phosphodiester bonding to the primer. The potential significance of the mineral catalyzed formation of 50 mer oligonucleotides to the origin of life based on RNA (the RNA world scenario) is discussed.

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Crossref · 10.1023/a:1020543312109

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4 of 4 references verified0 citation errors · 0 not checkable

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  • Montmorillonite clay can catalyze the polymerization of activated mononucleotides.

    “The ability of montmorillonite clay to catalyze the polymerization of activated mononucleotides is well known (Ferris et al. 1996;Ferris 2002).”

    From Protocells and RNA Self-Replication (2018) · cites this paper as Ferris 2002

Citations

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Research works printed but not linked: 19

  1. Ban, N., Nissen, P., Hansen, J., Moore, P. B. and Seitz, T. A.: 2000, The Complete Atomic Structure of the Large Ribosomal Subunit At 2.4 A Resolution, Science 289, 905–930. · DOI · Paper page
  2. Banin, A., Lawless, J. G., Mazzurco, J., Church, F. M., Margulies, L. and Orenberg, J. B.: 1985, Ph Profile of the Adsorption of Nucleotides onto Montmorillonite, Origins Life Evol. Biosphere 15, 89–101. · DOI
  3. Cech, T. R., Zaug, A. J. and Grabowski, P. J.: 1981, In Vitro Splicing of the Ribosomal RNA Precusor of Tetrahymena: Involvement of a Guanosine Nucleotide in the Excision of the Intervening Sequence, Cell 27, 487–496. · DOI
  4. Chu, B. C. F. and Orgel, L. E.: 1984, Preparation of Ligation Intermediates and Related Polynucleotide Pyrophosphates, Biochim. Biophys. Acta 782, 103–105. · DOI
  5. Crick, F. H. C.: 1968, The Origin of the Genetic Code, J. Mol. Biol. 38, 367–379. · DOI · Paper page
  6. Ding, Z. P., Kawamura, K. and Ferris, J. P.: 1996, Oligomerization of Uridine Phosphorimidazolides on Montmorillonite: A Model for the Prebiotic Synthesis of RNA on Minerals, Origins Life Evol. Biosphere 26, 151–171. · DOI
  7. Ertem, G. and Ferris, J. P.: 2000, Sequence-and Regio-Selectivity in the Montmorillonite-Catalyzed Synthesis of RNA, Origins Life Evol. Biosphere 30, 411–422. · DOI
  8. Ertem, G. and Ferris, J. P.: 1997, Template-Directed Synthesis Using the Heterogeneous Templates Produced by Montmorillonite Catalysis. A Possible Bridge Between the Prebiotic and RNA Worlds, J. Am. Chem. Soc. 119, 7197–7201. · DOI
  9. Ferris, J. P.: 1993, Catalysis and Prebiotic RNA Synthesis, Origins Life Evol. Biosphere 23, 307–315. · DOI
  10. Ferris, J. P. and Ertem, G.: 1993, Montmorillonite Catalysis of RNA Oligomer Formation in Aqueous Solution. A Model for the Prebiotic Formation of RNA, J. Am. Chem. Soc. 115, 12270–12275. · DOI
  11. Ferris, J. P.: 1985, Minerals and Life's Origins, Origins Life Evol. Biosphere 15, 75–76. · DOI
  12. Gilbert, W: 1986, The RNA World, Gilbert, Nature 319, 618. · DOI · Paper page
  13. Joyce, G. F. and Orgel, L. E.: 1999, Prospects for Understanding the Origin of the RNA World, in The RNA World: The Nature of Modern RNA Suggests a Prebiotic RNA; Second Ed.; Atkins, J. F., Ed.; Cold Spring Harbor Laboratory Press: Cold Spring Harbor, New York, pp. 49–77.
  14. Joyce, G. F.: 1998, Nucleic Acid Enzymes: Playing With a Fuller Deck, Proc. Natl. Acad. Sci. U.S.A. 95, 5845–5847. · DOI
  15. Muth, G. W., Ortoleva-Donnelly, L. and Strobel, S. A.: 2000, A Single Adenosine With A Neutral pk a in the Ribosomal Peptidyl Transferase Reaction Center, Science 289, 947–950. · DOI · Paper page
  16. Orgel, L. E.: 1968, Evolution of the Genetic Apparatus, J. Mol. Biol. 38, 381–393. · DOI · Paper page
  17. Orgel, L. E.: 1998, The Origin of Life-A Review of Facts and Speculation, TIBS 23, 491–495.
  18. Prabahar, K. J. and Ferris, J. P.: 1997, Adenine Derivatives As Phosphate-Activating Groups for the Regioselective formation of 3', 5'-Linked Oligoadenylates On Montmorillonite: Possible Phosphate-Activating Groups for the Prebiotic Synthesis of RNA, J. Am. Chem. Soc. 119, 4330–4337. · DOI
  19. Szostak, J. W. and Ellington, A. D.: 1993, In Vitro Selection of Functional RNA Sequences in The RNA World. The Nature of Modern RNA Suggests A Prebiotic RNA World; Atkins, J. F., Ed.; Cold Spring Harbor Laboratory Press: Cold Spring Harbor, New York, 1993; Vol. Monograph 24.

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Metadata

Authors
Ferris, J. P.
Affiliations
Rensselaer Polytechnic Institute (US)
Corresponding author
Ferris, James P.
Venue
Origins of life and evolution of the biosphere
Publisher
Springer Science and Business Media LLC
Published
2002-08
Volume
32
Issue
4
Pages
311-332
ISSN
  • 0169-6149 (ISSN-L)
  • 1573-0875
DOI
10.1023/a:1020543312109
OpenAlex
W2108421490
Cited by
183
References
4
Citations by year
2026: 5 · 2025: 1 · 2024: 4 · 2023: 1 · 2022: 5 · 2021: 8 · 2020: 6 · 2019: 4 · 2018: 5 · 2017: 13 · 2016: 3 · 2015: 8 · 2014: 7 · 2013: 11 · 2012: 11
FWCI
5.27
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • Origins of Life and Evolution of Biospheres (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Science Foundation
  • National Aeronautics and Space Administration
  • National Institutes of Health
  • Division of Chemistry
Topics
  • Physical Sciences › Physics and Astronomy › Astronomy and Astrophysics › Origins and Evolution of Life
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
Keywords
montmorillonite, clay catalysis, oligonucleotide synthesis, RNA world, origin of life, adenosine, uridine, primer elongation, alkaline phosphatase, ribonuclease T2, mineral catalysis
MeSH terms
Origin of Life, Adenosine, Alkaline Phosphatase, Bentonite, Catalysis, Endoribonucleases, Oligonucleotides, RNA, Time Factors, Uridine Monophosphate, Molecular Structure, Evolution, Chemical

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