Non-enzymatic template-directed synthesis on RNA random copolymers
Abstract
Poly(C, U) random copolymer templates direct the oligomerization of 2-MeImpG and 2-MeImpA, resulting in the production of a variety of oligo/(G,A)s. The efficiency of monomer incorporation into newly synthesized oligomers is greater for 2-MeImpG than for 2-MeImpA, and decreases for both monomers as the uracil content of the template increases. The relatively poor incorporation of adenine is partly due to an intrinsically less efficient incorporation reaction, and partly due to the masking of uracil sites by G X U non-complementary pairing. The efficiency of adenine incorporation can be improved by decreasing the concentration of 2-MeImpG and increasing the concentration of 2-MeImpA in the reaction mixture. The oligomeric product distribution can be characterized in detail using high-pressure liquid chromatography on an RPC-5 column. Oligomers are separated on the basis of chain length, base composition, and phospho-diester-linkage isomerism. The 3'----5' regiospecificity of monomer addition to template-bound oligomers is lower for 2-MeImpA than for 2-MeImpG. The presence of an adenine residue at the 2'(3') terminus of the acceptor strand lowers the regiospecificity of 2-MeImpA addition even further.
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Non-enzymatic RNA polymerization allows synthesis of short RNA oligonucleotides under prebiotic conditions.
“Non-enzymatic RNA polymerization allows synthesis of short RNA oligonucleotides under prebiotic conditions (Joyce et al., 1984; Adamala and Szostak, 2013b).”
From The Astrobiology Primer v2.0 (2016) · cites this paper as Joyce et al., 1984
The prebiotic replication of RNA could have occurred non-enzymatically inside protocells via chemically activated nucleotides.
“The prebiotic replication of RNA could have occurred non-enzymatically inside protocells via chemically activated nucleotides (Joyce et al., 1984; Mansy et al., 2008; Adamala and Szostak, 2013b).”
From The Astrobiology Primer v2.0 (2016) · cites this paper as Joyce et al., 1984
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Self-citationSynthetic Life with Alternative Nucleic Acids as Genetic Materials
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Synthesis and Nonenzymatic Template-Directed Polymerization of 2′-Amino-2′-deoxythreose Nucleotides
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Template-directed polynucleotide synthesis on mineral surfaces
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Self-citationMALDI analysis of oligonucleotides directly from montmorillonite
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Studies in the Mineral and Salt-Catalyzed Formation of RNA Oligomers
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Polyphosphorylation and Non-Enzymatic Template-Directed Ligation of Oligonucleotides
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Kopieren von RNA‐Sequenzen ohne Voraktivierung
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- Authors
- Joyce, G. F., Inoue, T., & Orgel, L. E.
- Affiliations
- Salk Institute for Biological Studies (US)
- ORCID
- Venue
- Journal of Molecular Biology
- Publisher
- Elsevier BV
- Published
- 1984-06
- Volume
- 176
- Issue
- 2
- Pages
- 279-306
- ISSN
- 0022-2836 (ISSN-L)
- OpenAlex
- W3184228035
- Cited by
- 178
- References
- 34
- Citations by year
- 2026: 1 · 2025: 4 · 2023: 3 · 2021: 2 · 2020: 4 · 2019: 5 · 2018: 2 · 2017: 4 · 2016: 8 · 2015: 6 · 2014: 5 · 2013: 4 · 2012: 5
- FWCI
- 2.51
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Molecular Biology (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Physical Sciences › Physics and Astronomy › Astronomy and Astrophysics › Origins and Evolution of Life
- Keywords
- oligonucleotide synthesis
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