Cited by 140DNA Repair MechanismsProtein Structure and DynamicsEnzyme Structure and Function

Solution structure of the DNA- and RPA-binding domain of the human repair factor XPA

Ikegami, Takahisa, Kuraoka, Isao, Saijo, Masafumi, Kodo, Naohiko, Kyogoku, Yoshimasa, Morikawa, Kosuke, Tanaka, Kiyoji, Shirakawa, Masahiro·1998·Nature Structural & Molecular Biology

Abstract

The solution structure of the central domain of the human nucleotide excision repair protein XPA, which binds to damaged DNA and replication protein A (RPA), was determined by nuclear magnetic resonance (NMR) spectroscopy. The central domain consists of a zinc-containing subdomain and a C-terminal subdomain. The zinc-containing subdomain has a compact globular structure and is distinct from the zinc-fingers found in transcription factors. The C-terminal subdomain folds into a novel alpha/beta structure with a positively charged superficial cleft. From the NMR spectra of the complexes, DNA and RPA binding surfaces are suggested.

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Crossref · 10.1038/1400

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Authors
Ikegami, T., Kuraoka, I., Saijo, M., Kodo, N., Kyogoku, Y., Morikawa, K., Tanaka, K., & Shirakawa, M.
Affiliations
  • Nara Institute of Science and Technology (JP)
  • The University of Osaka (JP)
  • Institute for Protein Research, The University of Osaka (JP)
  • Biomolecular Engineering Research Institute (Japan) (JP)
Corresponding author
Shirakawa, Masahiro
Venue
Nature Structural & Molecular Biology
Published
1998-08
Volume
5
Issue
8
Pages
701-706
ISSN
  • 1545-9985 (ISSN-L)
  • 1545-9993
OpenAlex
W2020761966
Cited by
139
References
27
Citations by year
2026: 2 · 2025: 1 · 2024: 3 · 2023: 4 · 2022: 3 · 2021: 2 · 2020: 4 · 2019: 8 · 2018: 5 · 2017: 8 · 2016: 7 · 2015: 4 · 2014: 9 · 2013: 2 · 2012: 2
FWCI
2.44
Type
journal article
Language
en
Open access
closed
Locations
  • Nature Structural & Molecular Biology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
  • Physical Sciences › Materials Science › Materials Chemistry › Enzyme Structure and Function
Keywords
XPA, nucleotide excision repair, DNA repair, replication protein A, DNA binding, alpha/beta fold, protein structure, solution structure, protein-DNA complex
MeSH terms
Amino Acid Sequence, Binding Sites, DNA Repair, DNA-Binding Proteins, Humans, Metalloproteins, Models, Molecular, Molecular Sequence Data, Protein Conformation, Xeroderma Pigmentosum, Zinc, RNA-Binding Proteins, Sequence Homology, Amino Acid, Nuclear Magnetic Resonance, Biomolecular, Replication Protein A, Xeroderma Pigmentosum Group A Protein

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