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Human aminopeptidase N is a receptor for human coronavirus 229E
Open accessAbstract
HUMAN coronaviruses (HCV) in two serogroups represented by HCV-229E and HCV-OC43 are an important cause of upper respiratory tract infections1. Here we report that human aminopeptidase N, a cell-surface metalloprotease on intestinal, lung and kidney epithelial cells2–5, is a receptor for human coronavirus strain HCV-229E, but not for HCV-OC43. A monoclonal antibody, RBS, blocked HCV-229E virus infection of human lung fibroblasts, immunoprecipitated aminopeptidase N and inhibited its enzymatic activity. HCV-229E-resistant murine fibroblasts became susceptible after transfection with complementary DNA encoding human aminopeptidase N. By contrast, infection of human cells with HCV-OC43 was not inhibited by antibody RBS and expression of aminopeptidase N did not enhance HCV-OC43 replication in mouse cells. A mutant aminopeptidase lacking the catalytic site of the enzyme did not bind HCV-229E or RBS and did not render murine cells susceptible to HCV-229E infection, suggesting that the virus-binding site may lie at or near the active site of the human aminopeptidase molecule.
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Metadata
- Authors
- Yeager, C. L., Ashmun, R. A., Williams, R. K., Cardellichio, C. B., Shapiro, L. H., Look, A. T., & Holmes, K. V.
- Venue
- Nature
- Published
- 1992-06-04
- Volume
- 357
- Issue
- 6377
- Pages
- 420-422
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W2027518730
- Cited by
- 941
- References
- 0
- FWCI
- 24.07
- Type
- journal article
- Language
- en
- Open access
- bronze
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