A Phagosome-to-Cytosol Pathway for Exogenous Antigens Presented on MHC Class I Molecules
Abstract
Peptides from endogenous proteins are presented by major histocompatibility complex class I molecules, but antigens (Ags) in the extracellular fluids are generally not. However, pathogens or particulate Ags that are internalized into phagosomes of macrophages (MØs) stimulate CD8 T cells. The presentation of these Ags is resistant to chloroquine but is blocked by inhibitors of the proteasome, a mutation in the TAP1-TAP2 transporter, and brefeldin A. Moreover, phagocytosis of a ribosomal-inactivating protein inhibited MØ protein synthesis. These results demonstrate that MØs transfer Ags from phagosomes into the cytosol and that endogenous and exogenous Ags use a final common pathway for class I presentation.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Melanoma cells and antigen-presenting cells can both display HLA-I and HLA-II peptides of bacterial origin.
“We demonstrate that bacterial HLA-I and HLA-II peptides can be presented by both antigen-presenting cells (as has previously been shown25–28) and by melanoma cells”
From Identification of bacteria-derived HLA-bound peptides in melanoma (2021) · cites this paper as [27]
Cites
Rock, K. L., Gramm, C., Rothstein, L., Clark, K., Stein, R., Dick, L., Hwang, D., & Goldberg, A. L. (1994) · Cell · doi:10.1016/s0092-8674(94)90462-6 · Cited by 2,524 · see citing works
Self-citationRole of Bone Marrow-Derived Cells in Presenting MHC Class I-Restricted Tumor Antigens
Huang, A. Y.-C., Golumbek, P. T., Ahmadzadeh, M., Jaffee, E. M., Pardoll, D. M., & Levitsky, H. I. (1994) · Science · doi:10.1126/science.7513904 · Cited by 1,159 · see citing works
Bevan, M. J. (1976) · The Journal of Experimental Medicine · doi:10.1084/jem.143.5.1283 · Cited by 962 · see citing works
Chakrabarti, S., Brechling, K., & Moss, B. A. (1985) · Molecular and Cellular Biology · doi:10.1128/mcb.5.12.3403 · Cited by 782 · see citing works
Kaer, L. V., Ashton‐Rickardt, P. G., Ploegh, H. L., & Tonegawa, S. (1992) · Cell · doi:10.1016/s0092-8674(05)80068-6 · Cited by 725 · see citing works
Phagocytic processing of bacterial antigens for class I MHC presentation to T cells
Pfeifer, J. D., Wick, M. J., Roberts, R. L., Findlay, K., Normark, S. J., & Harding, C. V. (1993) · Nature · doi:10.1038/361359a0 · Cited by 615 · see citing works
Kovacsovics-Bankowski, M., Clark, K., D, B. B. M., & Rock, K. L. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.11.4942 · Cited by 590 · see citing works
Self-citationProteolysis, proteasomes and antigen presentation
Goldberg, A. L., & Rock, K. L. (1992) · Nature · doi:10.1038/357375a0 · Cited by 576 · see citing works
Self-citationZiegler, H. K., & Unanue, E. R. (1982) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.79.1.175 · Cited by 570 · see citing works
Continuous proliferation of murine antigen-specific helper T lymphocytes in culture.
Watson, J. D. G. (1979) · The Journal of Experimental Medicine · doi:10.1084/jem.150.6.1510 · Cited by 518 · see citing works
Spies, T. A., & DeMars, R. (1991) · Nature · doi:10.1038/351323a0 · Cited by 413 · see citing works
Class I-restricted processing and presentation of exogenous cell-associated antigen in vivo.
Carbone, F., & Bevan, M. J. (1990) · The Journal of Experimental Medicine · doi:10.1084/jem.171.2.377 · Cited by 407 · see citing works
Yewdell, J. W., & Bennink, J. R. (1992) · Advances in immunology · doi:10.1016/s0065-2776(08)60875-5 · Cited by 362 · see citing works
CD8+ T lymphocytes in intracellular microbial infections
Kaufmann, S. H. E. (1988) · Immunology Today · doi:10.1016/0167-5699(88)91292-3 · Cited by 332 · see citing works
Brefeldin A Specifically Inhibits Presentation of Protein Antigens to Cytotoxic T Lymphocytes
Yewdell, J. W., & Bennink, J. R. (1989) · Science · doi:10.1126/science.2471266 · Cited by 328 · see citing works
Stirpe, F., Olsnes, S., & Pihl, A. (1980) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)43667-8 · Cited by 318 · see citing works
Cytotoxic T lymphocytes against a soluble protein
Staerz, U. D., Karasuyama, H., & Garner, A. M. (1987) · Nature · doi:10.1038/329449a0 · Cited by 171 · see citing works
Bacik, I., Cox, J. H., Anderson, R., et al. (1994) · The Journal of Immunology · doi:10.4049/jimmunol.152.2.381 · Cited by 145 · see citing works
Lambert, J. M., Senter, P. D., Yau-Young, A., et al. (1985) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)38981-0 · Cited by 138 · see citing works
Furuno, K., & Goldberg, A. L. (1986) · Biochemical Journal · doi:10.1042/bj2370859 · Cited by 123 · see citing works
The transporters associated with antigen presentation
Townsend, A., & Trowsdale, J. (1993) · Seminars in Cell Biology · doi:10.1006/scel.1993.1007 · Cited by 117 · see citing works
Magdalena, M., Kovacsovics‐Bankowski, & Rock, K. L. (1994) · European Journal of Immunology · doi:10.1002/eji.1830241024 · Cited by 105 · see citing works
Self-citationVacuolar/lysosomal proteolysis: proteases, substrates mechanisms
Knop, M., Schiffer, H. H., Rupp, S., & Wolf, D. H. (1993) · Current Opinion in Cell Biology · doi:10.1016/0955-0674(93)90082-2 · Cited by 93 · see citing works
Generation of class I MHC-restricted T-T hybridomas.
Rock, K. L., Rothstein, L., & Gamble, S. (1990) · The Journal of Immunology · doi:10.4049/jimmunol.145.3.804 · Cited by 84 · see citing works
Self-citationThe involvement of herpes simplex virus type 1 glycoproteins in cell-mediated immunity.
Carter, V. C., Schaffer, P. A., & Tevethia, S. S. (1981) · The Journal of Immunology · doi:10.4049/jimmunol.126.5.1655 · Cited by 82 · see citing works
Szalay, G., Hess, J., & Kaufmann, S. H. E. (1994) · European Journal of Immunology · doi:10.1002/eji.1830240703 · Cited by 39 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Merad, M., Sathe, P., Helft, J., Miller, J., & Mortha, A. (2013) · Annual Review of Immunology · doi:10.1146/annurev-immunol-020711-074950 · Cited by 2,309 · see citing works
The Liver as an Immunological Organ
Racanelli, V., & Rehermann, B. (2006) · Hepatology · doi:10.1002/hep.21060 · Cited by 1,377 · see citing works
Present yourself! by MHC class I and MHC class II molecules
Rock, K. L., Reits, E., & Neefjes, J. (2016) · Trends in Immunology · doi:10.1016/j.it.2016.08.010 · Cited by 814 · see citing works
Self-citationStuart, L. M., & Ezekowitz, R. A. B. (2005) · Immunity · doi:10.1016/j.immuni.2005.05.002 · Cited by 667 · see citing works
Identification of bacteria-derived HLA-bound peptides in melanoma
Kalaora, S., Nagler, A., Nejman, D., Alon, M., Barbolin, C., Barnea, E., Ketelaars, S. L. C., Cheng, K., Vervier, K., Shental, N., et al. (2021) · Nature · doi:10.1038/s41586-021-03368-8 · Cited by 457 · see citing works
Biomaterials and emerging anticancer therapeutics: engineering the microenvironment
Gu, L., & Mooney, D. J. (2016) · Nature Reviews Cancer · doi:10.1038/nrc.2015.3 · Cited by 407 · see citing works
Alum interaction with dendritic cell membrane lipids is essential for its adjuvanticity
Flach, T. L., Ng, G., Hari, A., Desrosiers, M. D., Zhang, P., Ward, S. M., Seamone, M. E., Vilaysane, A., Mucsi, A. D., Fong, Y., et al. (2011) · Nature Medicine · doi:10.1038/nm.2306 · Cited by 405 · see citing works
Inflammasome-activating nanoparticles as modular systems for optimizing vaccine efficacy
Demento, S. L., Eisenbarth, S. C., Foellmer, H. G., Platt, C., Caplan, M. J., Mark Saltzman, W., Mellman, I., Ledizet, M., Fikrig, E., Flavell, R. A., et al. (2009) · Vaccine · doi:10.1016/j.vaccine.2009.03.034 · Cited by 290 · see citing works
Ng, G., Sharma, K., Ward, S. M., Desrosiers, M. D., Stephens, L. A., Schoel, W. M., Li, T., Lowell, C. A., Ling, C.-C., Amrein, M. W., et al. (2008) · Immunity · doi:10.1016/j.immuni.2008.09.013 · Cited by 276 · see citing works
Lecoultre, M., Dutoit, V., & Walker, P. R. (2020) · Journal for ImmunoTherapy of Cancer · doi:10.1136/jitc-2020-001408 · Cited by 223 · see citing works
Antigen processing and presentation
(2019) · International Review of Cell and Molecular Biology · doi:10.1016/bs.ircmb.2019.07.005 · Cited by 194 · see citing works
Moghimi, S. M. (2002) · Biochimica et Biophysica Acta (BBA) - Molecular Cell Research · doi:10.1016/s0167-4889(02)00204-5 · Cited by 103 · see citing works
T Cell-Mediated Immune Responses to AAV and AAV Vectors
Ertl, H. C. J. (2021) · Frontiers in Immunology · doi:10.3389/fimmu.2021.666666 · Cited by 88 · see citing works
Kuchtey, J., Chefalo, P. J., Gray, R. C., Ramachandra, L., & Harding, C. V. (2005) · The Journal of Immunology · doi:10.4049/jimmunol.175.4.2244 · Cited by 66 · see citing works
- Authors
- Kovacsovics-Bankowski, M., & Rock, K. L.
- Affiliations
- Dana-Farber Cancer Institute (US)
- Harvard University (US)
- Venue
- Science
- Published
- 1995-01-13
- Volume
- 267
- Issue
- 5195
- Pages
- 243-246
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2079439306
- Cited by
- 884
- References
- 26
- Citations by year
- 2026: 6 · 2025: 6 · 2024: 7 · 2023: 24 · 2022: 14 · 2021: 11 · 2020: 24 · 2019: 18 · 2018: 21 · 2017: 13 · 2016: 17 · 2015: 21 · 2014: 21 · 2013: 24 · 2012: 32
- FWCI
- 23.36
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r01ai031337-08)
- National Institute of Allergy and Infectious Diseases (AI-31337, AI-20248)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › vaccines and immunoinformatics approaches
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Keywords
- MHC class I antigen presentation, exogenous antigen, macrophages, phagocytosis, CD8+ T cells, chloroquine resistance, proteasome inhibitors, brefeldin A, protein synthesis, endogenous antigen
- MeSH terms
- ATP Binding Cassette Transporter, Subfamily B, Member 2, ATP Binding Cassette Transporter, Subfamily B, Member 3, Amino Acid Sequence, Animals, Antigens, Cell Line, Chloroquine, Cyclopentanes, Cysteine Endopeptidases, Cytosol, Macrophages, Molecular Sequence Data, Multienzyme Complexes, Oligopeptides, Ovalbumin, Phagocytosis, Phagosomes, Plant Proteins, Histocompatibility Antigens Class I, Antigen Presentation, ATP-Binding Cassette Transporters, Brefeldin A, Proteasome Endopeptidase Complex, Mice, Ribosome Inactivating Proteins, Type 1
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review