Cited by 884Immunotherapy and Immune Responsesvaccines and immunoinformatics approachesCellular transport and secretion

A Phagosome-to-Cytosol Pathway for Exogenous Antigens Presented on MHC Class I Molecules

Kovacsovics-Bankowski, M., Rock, K. L.·1995·Science

Abstract

Peptides from endogenous proteins are presented by major histocompatibility complex class I molecules, but antigens (Ags) in the extracellular fluids are generally not. However, pathogens or particulate Ags that are internalized into phagosomes of macrophages (MØs) stimulate CD8 T cells. The presentation of these Ags is resistant to chloroquine but is blocked by inhibitors of the proteasome, a mutation in the TAP1-TAP2 transporter, and brefeldin A. Moreover, phagocytosis of a ribosomal-inactivating protein inhibited MØ protein synthesis. These results demonstrate that MØs transfer Ags from phagosomes into the cytosol and that endogenous and exogenous Ags use a final common pathway for class I presentation.

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Crossref · 10.1126/science.7809629

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  • Melanoma cells and antigen-presenting cells can both display HLA-I and HLA-II peptides of bacterial origin.

    “We demonstrate that bacterial HLA-I and HLA-II peptides can be presented by both antigen-presenting cells (as has previously been shown25–28) and by melanoma cells”

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Authors
Kovacsovics-Bankowski, M., & Rock, K. L.
Affiliations
  • Dana-Farber Cancer Institute (US)
  • Harvard University (US)
Venue
Science
Published
1995-01-13
Volume
267
Issue
5195
Pages
243-246
ISSN
  • 0036-8075 (ISSN-L)
  • 1095-9203
OpenAlex
W2079439306
Cited by
884
References
26
Citations by year
2026: 6 · 2025: 6 · 2024: 7 · 2023: 24 · 2022: 14 · 2021: 11 · 2020: 24 · 2019: 18 · 2018: 21 · 2017: 13 · 2016: 17 · 2015: 21 · 2014: 21 · 2013: 24 · 2012: 32
FWCI
23.36
Type
journal article
Language
en
Open access
closed
Locations
  • Science (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (5r01ai031337-08)
  • National Institute of Allergy and Infectious Diseases (AI-31337, AI-20248)
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › vaccines and immunoinformatics approaches
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
Keywords
MHC class I antigen presentation, exogenous antigen, macrophages, phagocytosis, CD8+ T cells, chloroquine resistance, proteasome inhibitors, brefeldin A, protein synthesis, endogenous antigen
MeSH terms
ATP Binding Cassette Transporter, Subfamily B, Member 2, ATP Binding Cassette Transporter, Subfamily B, Member 3, Amino Acid Sequence, Animals, Antigens, Cell Line, Chloroquine, Cyclopentanes, Cysteine Endopeptidases, Cytosol, Macrophages, Molecular Sequence Data, Multienzyme Complexes, Oligopeptides, Ovalbumin, Phagocytosis, Phagosomes, Plant Proteins, Histocompatibility Antigens Class I, Antigen Presentation, ATP-Binding Cassette Transporters, Brefeldin A, Proteasome Endopeptidase Complex, Mice, Ribosome Inactivating Proteins, Type 1

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