The DOI matches its Crossref record; 27 of the 27 references it cites were found in a registry or an index.
Abnormal avoidance learning in mice lacking functional high-affinity nicotine receptor in the brain
Abstract
Nicotine affects many aspects of behaviour including learning and memory through its interaction with neuronal nicotinic acetylcholine receptors (nAChR). Functional nAChRs are pentameric proteins containing at least one type of alpha-subunit and one type of beta-subunit. The involvement of a particular neuronal nicotinic subunit in pharmacology and behaviour was examined using gene targeting to mutate beta 2, the most widely expressed nAChR subunit in the central nervous system. We report here that high-affinity binding sites for nicotine are absent from the brains of mice homozygous for the beta 2-subunit mutation. Further, electrophysiological recording from brain slices reveals that thalamic neurons from these mice do not respond to nicotine application. Finally, behavioural tests demonstrate that nicotine no longer augments the performance of beta 2-1- mice on passive avoidance, a test of associative memory. Paradoxically, mutant mice are able to perform better than their non-mutant siblings on this task.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“The β2* nAChRs are thought to have the highest affinity for nicotine and other agonists (Lippiello et al., 1987; McGehee and Role, 1995; Picciotto et al., 1995) while α7* nAChRs are thought to have a lower affinity for nicotine in vitro (Papke and Thinschmidt, 1998; Wonnacott, 1986).”
From It is not “either/or”: Activation and desensitization of nicotinic acetylcholine receptors both contribute to behaviors related to nicotine addiction and mood (2008) · cites this paper as Picciotto et al., 1995
In the citing paper
“cytisine, a nicotinic partial agonist that blocks α4/β2* nAChRs but activates α3/β4* and α7* nAChRs (Papke and Porter Papke, 2002; Picciotto et al., 1995)”
From It is not “either/or”: Activation and desensitization of nicotinic acetylcholine receptors both contribute to behaviors related to nicotine addiction and mood (2008) · cites this paper as Picciotto et al., 1995
In the citing paper
“the hypersensitive passive avoidance performance behavior exhibited by β2 knockout mice [33] can be normalized by transgenic expression of this subunit exclusively in corticothalamic neurons during development [27, 28].”
From Impaired auditory discrimination learning following perinatal nicotine exposure or β2 nicotinic acetylcholine receptor subunit deletion (2012) · cites this paper as [33]
Citations
Cites
Molecular Cloning: A Laboratory Manual
Sambrook, J., Fritsch, E., & Maniatis, T. (1989) · UCLA - Biblioteca de Ciencias de la Salud · Cited by 133,589
Molecular cloning: A laboratory manual
Deininger, P. L. (1990) · Analytical Biochemistry · doi:10.1016/0003-2697(90)90595-z · Cited by 84,684
Impaired Spatial Learning in α-Calcium-Calmodulin Kinase II Mutant Mice
Silva, A. J., Paylor, R. E., Wehner, J. M., & Tonegawa, S. (1992) · Science · doi:10.1126/science.1321493 · Cited by 1,366
The Diversity of Neuronal Nicotinic Acetylcholine Receptors
Sargent, P. B. (1993) · Annual Review of Neuroscience · doi:10.1146/annurev.ne.16.030193.002155 · Cited by 1,052
Wada, E., Wada, K., Boulter, J., Deneris, E. S., Heinemann, S., Patrick, J., & Swanson, L. W. (1989) · The Journal of Comparative Neurology · doi:10.1002/cne.902840212 · Cited by 1,044
Clarke, P. B. S., Schwartz, R., Paul, S. M., Cb, P., & Pert, A. (1985) · Journal of Neuroscience · doi:10.1523/jneurosci.05-05-01307.1985 · Cited by 1,037
Morris, R. (1989) · Journal of Neuroscience · doi:10.1523/jneurosci.09-09-03040.1989 · Cited by 770
Exploratory behavior models of anxiety in mice
Crawley, J. N. (1985) · Neuroscience & Biobehavioral Reviews · doi:10.1016/0149-7634(85)90030-2 · Cited by 756
Luetje, C., & Patrick, J. (1991) · The Journal of Neuroscience · doi:10.1523/jneurosci.11-03-00837.1991 · Cited by 558
Nicotine binding and nicotinic receptor subunit RNA after chronic nicotine treatment
Marks, M., Pauly, Jr., Gross, S., Deneris, E., Hermans-Borgmeyer, I., Heinemann, S., & Collins, A. (1992) · The Journal of Neuroscience · doi:10.1523/jneurosci.12-07-02765.1992 · Cited by 551
Nicotinic systems and cognitive function
Levin, E. D. (1992) · Psychopharmacology · doi:10.1007/bf02247415 · Cited by 511
Flicker, C., DEAN, R., Watkins, D. L., FISHER, S., & Bartus, R. T. (1983) · Pharmacology Biochemistry and Behavior · doi:10.1016/s0091-3057(83)80023-9 · Cited by 370
Homeosis in the mouse induced by a null mutation in the Hox-3.1 gene
Mouellic, H. L., Lallemand, Y., & Brûlet, P. (1992) · Cell · doi:10.1016/0092-8674(92)90406-3 · Cited by 356
Self-citationFunctional Architecture Of The Nicotinic Acetylcholine Receptor: From Electric Organ To Brain
Galzi, J., Revah, F., Bessis, A., & Changeux, J. (1991) · The Annual Review of Pharmacology and Toxicology · doi:10.1146/annurev.pa.31.040191.000345 · Cited by 332
Self-citationStereospecific Nicotine Receptors on Rat Brain Membranes
Romano, C., & D, A. G. M. (1980) · Science · doi:10.1126/science.7433991 · Cited by 318
Anand, R., Conroy, W. G., Schoepfer, R., Whiting, P. J., & Lindstrom, J. M. (1991) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)99147-7 · Cited by 315
A new mouse embryonic stem cell line with good germ line contribution and gene targeting frequency
Magin, T. M., McWhir, J., & Melton, D. W. (1992) · Nucleic Acids Research · doi:10.1093/nar/20.14.3795 · Cited by 246
Yagi, T., Ikawa, Y., Yoshida, K., Shigetani, Y., Takeda, N., Mabuchi, I., Yamamoto, T., & Aizawa, S. (1990) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.87.24.9918 · Cited by 238
Immunocytochemical localization of a neuronal nicotinic receptor: the beta 2-subunit
Hill, J., Zoli, M., Bourgeois, J., & Changeux, J. (1993) · The Journal of Neuroscience · doi:10.1523/jneurosci.13-04-01551.1993 · Cited by 220
Self-citationDecker, M. W., Brioni, J. D., Sullivan, J. P., Buckley, M. J., Radek, R. J., Raszkiewicz, J. L., Kang, C. H., Kim, D. J., Giardina, W. J., & Wasicak, J. T. (1994) · The Journal of Pharmacology and Experimental Therapeutics · doi:10.1016/s0022-3565(25)22339-4 · Cited by 180
Selfridge, J., Pow, A. M., McWhir, J., Magin, T. M., & Melton, D. W. (1992) · Somatic Cell and Molecular Genetics · doi:10.1007/bf01235756 · Cited by 110
Swanson, L. W., Lindstrom, J. M., Tzartos, S. J., Schmued, L. C., O'Leary, D. D. M., & Cowan, W. M. (1983) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.80.14.4532 · Cited by 105
(S)‐3‐メチル‐5‐(1‐メチル‐2‐ピロリジニル)イソキサゾール(ART418) 認知促進および抗不安活性を持つ新しいコリン作動性リガンド II in vivoの性質
Decker, M. W., Brioni, J. D., Sullivan, J. P., et al. (1994) · Journal of Pharmacology and Experimental Therapeutics · Cited by 92
EFFECTS OF MECAMYLAMINE ON AVOIDANCE CONDITIONING AND MAZE LEARNING OF MICE
Oliverio, A. (1966) · Journal of Pharmacology and Experimental Therapeutics · doi:10.1016/s0022-3565(25)27477-8 · Cited by 61
A two-compartment exploratory model to study anxiolytic/anxiogenic effects of drugs in the rat
Pich, E. M., & SAMANIN, R. (1989) · Pharmacological Research · doi:10.1016/1043-6618(89)90201-6 · Cited by 52
Self-citationEffect of Nicotine on Passive Avoidance Behaviour and Motoric Activity in Mice
Nordberg, A. K., & Bergh, C. (1985) · Acta Pharmacologica et Toxicologica · doi:10.1111/j.1600-0773.1985.tb01300.x · Cited by 45
Faiman, C. P., de Erausquin, G. A., & Baratti, C. M. (1991) · Behavioral and Neural Biology · doi:10.1016/0163-1047(91)90592-e · Cited by 28
Flagged first, then by citations
Research works printed but not linked: 5
- Zoli, M., Le Novère, N., Hill, J. A. J. & Changeux, J.-P. J. Neurosci. (in the press).
- Flores, C. M., Rogers, S. W., Pabreza, L. A., Wolfe, B. B. & Kellar, K. J. Molec. Pharm. 41, 31–37 (1992).
- Marks, M. J. & Collins, A. C. Molec. Pharm. 22, 554–564 (1982).
- Decker, M. W. et al. J. pharmacol. exp. Ther. 270, 319–328 (1994).
- Oliverio, A. J. pharmacol. exp. Ther. 154, 350–356 (1966).
Cited by
Sorted by citations
Acetylcholine as a Neuromodulator: Cholinergic Signaling Shapes Nervous System Function and Behavior
Picciotto, M. R., Higley, M. J., & Mineur, Y. S. (2012) · Neuron · doi:10.1016/j.neuron.2012.08.036 · Cited by 1,404
Self-citationPicciotto, M. R., Zoli, M., Rimondini, R., Léna, C., Marubio, L. M., Pich, E. M., Fuxe, K., & Changeux, J.-P. (1998) · Nature · doi:10.1038/34413 · Cited by 1,320
Self-citationNicotinic Acetylcholine Receptors and Nicotinic Cholinergic Mechanisms of the Central Nervous System
Dani, J. A., & Bertrand, D. (2007) · Annual Review of Pharmacology and Toxicology · doi:10.1146/annurev.pharmtox.47.120505.105214 · Cited by 1,197
Neuronal nicotinic receptors: from structure to pathology
Gotti, C., & Clementi, F. (2004) · Progress in Neurobiology · doi:10.1016/j.pneurobio.2004.09.006 · Cited by 970
Gene-targeting studies of mammalian behavior: is it the mutation or the background genotype?
Gerlai, R. (1996) · Trends in Neurosciences · doi:10.1016/s0166-2236(96)20020-7 · Cited by 881
Metadata
- Authors
- Picciotto, M. R., Zoli, M., Léna, C., Bessis, A., Lallemand, Y., LeNovère, N., Vincent, P., Pich, E. M., Brûlet, P., & Changeux, J.-P.
- Affiliations
- Centre National de la Recherche Scientifique (FR)
- Venue
- Nature
- Published
- 1995-03
- Volume
- 374
- Issue
- 6517
- Pages
- 65-67
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W1996157865
- Cited by
- 668
- References
- 26
- Citations by year
- 2026: 2 · 2025: 4 · 2023: 9 · 2022: 27 · 2021: 8 · 2020: 15 · 2019: 12 · 2018: 10 · 2017: 12 · 2016: 17 · 2015: 16 · 2014: 18 · 2013: 20 · 2012: 27
- FWCI
- 10.67
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nicotinic Acetylcholine Receptors Study
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Smoking Behavior and Cessation
- Keywords
- nicotinic acetylcholine receptors, beta2 subunit, avoidance learning, passive avoidance, associative memory, thalamic neurons, nicotine binding, gene targeting
- MeSH terms
- Animals, Avoidance Learning, Base Sequence, Brain, Cell Line, DNA, Female, Homozygote, Hypothalamus, Male, Mice, Inbred C57BL, Mice, Inbred DBA, Molecular Sequence Data, Mutation, Nicotine, Receptors, Nicotinic, Patch-Clamp Techniques, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
