The DOI matches its Crossref record; 27 of the 27 references it cites were found in a registry or an index.
Abnormal avoidance learning in mice lacking functional high-affinity nicotine receptor in the brain
Abstract
Nicotine affects many aspects of behaviour including learning and memory through its interaction with neuronal nicotinic acetylcholine receptors (nAChR). Functional nAChRs are pentameric proteins containing at least one type of alpha-subunit and one type of beta-subunit. The involvement of a particular neuronal nicotinic subunit in pharmacology and behaviour was examined using gene targeting to mutate beta 2, the most widely expressed nAChR subunit in the central nervous system. We report here that high-affinity binding sites for nicotine are absent from the brains of mice homozygous for the beta 2-subunit mutation. Further, electrophysiological recording from brain slices reveals that thalamic neurons from these mice do not respond to nicotine application. Finally, behavioural tests demonstrate that nicotine no longer augments the performance of beta 2-1- mice on passive avoidance, a test of associative memory. Paradoxically, mutant mice are able to perform better than their non-mutant siblings on this task.
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In the citing paper
“The β2* nAChRs are thought to have the highest affinity for nicotine and other agonists (Lippiello et al., 1987; McGehee and Role, 1995; Picciotto et al., 1995) while α7* nAChRs are thought to have a lower affinity for nicotine in vitro (Papke and Thinschmidt, 1998; Wonnacott, 1986).”
From It is not “either/or”: Activation and desensitization of nicotinic acetylcholine receptors both contribute to behaviors related to nicotine addiction and mood (2008) · cites this paper as Picciotto et al., 1995
In the citing paper
“cytisine, a nicotinic partial agonist that blocks α4/β2* nAChRs but activates α3/β4* and α7* nAChRs (Papke and Porter Papke, 2002; Picciotto et al., 1995)”
From It is not “either/or”: Activation and desensitization of nicotinic acetylcholine receptors both contribute to behaviors related to nicotine addiction and mood (2008) · cites this paper as Picciotto et al., 1995
In the citing paper
“the hypersensitive passive avoidance performance behavior exhibited by β2 knockout mice [33] can be normalized by transgenic expression of this subunit exclusively in corticothalamic neurons during development [27, 28].”
From Impaired auditory discrimination learning following perinatal nicotine exposure or β2 nicotinic acetylcholine receptor subunit deletion (2012) · cites this paper as [33]
Citations
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Acetylcholine as a Neuromodulator: Cholinergic Signaling Shapes Nervous System Function and Behavior
Picciotto, M. R., Higley, M. J., & Mineur, Y. S. (2012) · Neuron · doi:10.1016/j.neuron.2012.08.036 · Cited by 1,404
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Self-citationNicotinic Acetylcholine Receptors and Nicotinic Cholinergic Mechanisms of the Central Nervous System
Dani, J. A., & Bertrand, D. (2007) · Annual Review of Pharmacology and Toxicology · doi:10.1146/annurev.pharmtox.47.120505.105214 · Cited by 1,197
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Gotti, C., & Clementi, F. (2004) · Progress in Neurobiology · doi:10.1016/j.pneurobio.2004.09.006 · Cited by 970
Gene-targeting studies of mammalian behavior: is it the mutation or the background genotype?
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Metadata
- Authors
- Picciotto, M. R., Zoli, M., Léna, C., Bessis, A., Lallemand, Y., LeNovère, N., Vincent, P., Pich, E. M., Brûlet, P., & Changeux, J.-P.
- Affiliations
- Centre National de la Recherche Scientifique (FR)
- Venue
- Nature
- Published
- 1995-03
- Volume
- 374
- Issue
- 6517
- Pages
- 65-67
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W1996157865
- Cited by
- 668
- References
- 26
- Citations by year
- 2026: 2 · 2025: 4 · 2023: 9 · 2022: 27 · 2021: 8 · 2020: 15 · 2019: 12 · 2018: 10 · 2017: 12 · 2016: 17 · 2015: 16 · 2014: 18 · 2013: 20 · 2012: 27
- FWCI
- 10.67
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nicotinic Acetylcholine Receptors Study
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Smoking Behavior and Cessation
- Keywords
- nicotinic acetylcholine receptors, beta2 subunit, avoidance learning, passive avoidance, associative memory, thalamic neurons, nicotine binding, gene targeting
- MeSH terms
- Animals, Avoidance Learning, Base Sequence, Brain, Cell Line, DNA, Female, Homozygote, Hypothalamus, Male, Mice, Inbred C57BL, Mice, Inbred DBA, Molecular Sequence Data, Mutation, Nicotine, Receptors, Nicotinic, Patch-Clamp Techniques, Mice
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