Immunocytochemical localization of a neuronal nicotinic receptor: the beta 2-subunit
Open accessAbstract
We have characterized in adult rat the tissue-specific expression of the nicotinic ACh receptor (AChR) beta 2-subunit using antisera raised against fusion protein constructs. Immunohistochemical localization revealed immunoreactivity distributed throughout the neuraxis. Overall, beta 2-like immunoreactivity (beta 2-LI) correlated well with in situ localization of beta 2 transcript in neuronal cell bodies. Particularly strong labeling was detected in the thalamus, and scattered other regions, whereas relatively weak staining was observed in the hypothalamus and amygdala. At the cellular level, beta 2-LI appeared to be exclusively neuronal and concentrated predominantly in perikarya, although strongly positive dendrites (cerebral cortical pyramidal neurons, cerebellar Purkinje cells) and axon terminals (e.g., striatum) were detected. At the ultrastructural level, beta 2-LI was membrane associated, with strong staining observed in endoplasmic reticulum and cytoplasmic transport vesicles. beta 2-LI was rarely detected at synapses. The widespread distribution of beta 2 suggests it may serve as a common subunit in different AChR combinations in various brain regions. Regulation of the expression of beta 2-subunit appears to be relatively unrestrained, with an apparent excess of protein synthesized in the cytoplasm relative to that which ultimately arrives at functional targets in the plasma membrane.
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Endogenous α4β2 nAChRs localize at least partially to the smooth ER in mouse dorsal raphe and rat cortical neurons.
“Electron microscopy studies in mouse dorsal raphe and rat cortical neurons show that endogenous α4β2 nAChRs localize at least partially to the smooth ER (Hill et al., 1993; Commons, 2008).”
From Nicotine up-regulates α4β2 nicotinic receptors and ER exit sites via stoichiometry-dependent chaperoning (2011) · cites this paper as Hill et al., 1993
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- Authors
- Hill, J., Zoli, M., Bourgeois, J., & Changeux, J.
- Affiliations
- Centre National de la Recherche Scientifique (FR)
- ORCID
- Zoli, M
- Venue
- The Journal of Neuroscience
- Publisher
- Society for Neuroscience
- Published
- 1993-04-01
- Volume
- 13
- Issue
- 4
- Pages
- 1551-1568
- ISSN
- 0270-6474 (ISSN-L)
- 1529-2401
- PMC
- PMC6576729
- OpenAlex
- W1926699758
- Cited by
- 220
- References
- 30
- Citations by year
- 2025: 3 · 2024: 3 · 2022: 1 · 2021: 3 · 2020: 3 · 2019: 3 · 2018: 5 · 2017: 2 · 2016: 2 · 2015: 5 · 2014: 7 · 2013: 8 · 2012: 7
- FWCI
- 4.25
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Neuroscience (publishedVersion, cc-by-nc-sa)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- European Molecular Biology Organization
- Centre National de la Recherche Scientifique
- National Institutes of Health
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nicotinic Acetylcholine Receptors Study
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Receptor Mechanisms and Signaling
- Keywords
- nicotinic acetylcholine receptors, beta2 subunit, subunit expression, thalamus, hypothalamus, amygdala, neuronal localization, dendrites, axon terminals, Purkinje cells, pyramidal neurons, striatum, endoplasmic reticulum, transport vesicles, rat brain
- MeSH terms
- Amino Acid Sequence, Animals, Base Sequence, Brain, Immunohistochemistry, Molecular Sequence Data, Neurons, Receptors, Nicotinic, Recombinant Fusion Proteins, Tissue Distribution, Molecular Probes, Rats, Sprague-Dawley, In Situ Hybridization, Rats
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